Increased Synthesis of the Phosphorylated Form of the Myosin Light Chains in Cardiac Hypertrophy in the Rat

  • B. Kwiatkowska-Patzer
  • G. Prior
  • R. Zak

Abstract

The incorporation rate of [3H]leucine into cardiac myosin subunits was studied in rat hearts undergoing hypertrophy secondary to constriction of the ascending aorta. Cardiac myosin was prepared by a modified Shiverick’s method on the second and fourth day after constriction. Myosin light chains were separated by urea and subjected to two-dimensional electrophoresis. Incorporation of [3H]leucine was determined in electrophoretically separated heavy and light chains by the method of Martin et al. (11). It was found that the incorporation rate of [3H]leucine into the phosphorylated form of the myosin light chain 2 is significantly increased in hypertrophic hearts as compared to sham animals.

Keywords

Light Chain Myosin Light Chain Phosphorylated Form Specific Radioactivity Myosin Light Chain Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • B. Kwiatkowska-Patzer
    • 1
  • G. Prior
    • 1
  • R. Zak
    • 1
  1. 1.Department of MedicineUniversity of ChicagoChicagoUSA

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