Abstract
This investigation was designed to evaluate the difference in DNA activity between biopsy specimens obtained from right and left ventricles. Nucleic DNA in the myocardial cells of hypertrophied and congestive forms of cardiomyopathy was analyzed to investigate the relationship between cell function and clinical manifestations. Endomyocardial biopsy specimens were obtained simultaneously from right ventricular septal wall and left ventricular in-ferolateral wall by a transcatheter biotome. Measurement of DNA was based on the Feulgen reaction and dual wavelength cytophotometry. In this series, 12 patients with hypertrophic cardiomyopathy and four patients with congestive cardiomyopathy were studied. In the normal heart, the DNA value (arbitrary units) of the right ventricle was 138.6, whereas that of the left ventricle was 144.4. In the hypertrophic group, the mean DNA value in the right ventricle was 279.9, whereas that of the left ventricle was 317.5. In the congestive group, the DNA mean value in the right ventricle was 108.8, whereas that of left ventricle was 144.8. The linear relationship (r = 0.67) between right and left ventricular DNA values suggests that cellular function of one ventricle is affected by that of the other side. Higher DNA values of the left ventricle may indicate the difference in work load between the ventricles. The relationships among DNA values, LV wall thickness, LV mass, and parameters of contractility were statistically high.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abe, H., and Kitamura, K. 1981. New trial of myocardial biopsy. Jpn. Circ. J. 45 (Suppl.) 22.
Brooksby, A. B., Jenkins, B. S., Coltart, D. J., and Webb-Peploe, M. M. 1974. Left ventricular endomyocardial biopsy. Lancet 2:1222–1225.
Konno, S., Sekiguchi, M., and Sakakibara, S. 1971. Catheter biopsy of the heart. Radiol. Clin. North Am. 9:491–510.
Kunkel, B., Lapp, H., Kober, G., and Kaltenbach, M. 1978. Light-microscopic evaluation of myocardial biopsies. In: M. Kaltenbach, F. Loogen, and E. G. Olsen (eds.), Cardiomyopathy and Myocardial Biopsy, pp. 62–70. Springer-Verlag, Berlin, Heidelberg, New York.
Sandritter, W., and Scomazzoni, G. 1964. DNA content (Feulgen photometry) and dry weight (interference microscopy) of normal and hypertrophic heart muscle fibres. Nature 202:100–115.
Shirey, E. K., Hawk, W. A., Mukerji, D., and Effler, D. B. 1972. Percutaneous myocardial biopsy of the left ventricle. Experience in 198 patients. Circulation 46:112–122.
Yabe, Y., and Abe, H. 1979. Changes in DNA and RNA synthesis of the cardiac muscle cell in patients with cardiomyopathy (myocardial biopsy). J. Mol. Cell. Cardiol. 77(Suppl. 7):68.
Yabe, Y., and Abe, H. 1980. Changes in DNA synthesis in significantly hypertrophied human cardiac muscle. In: M. Tajuddin, P. K. Das, M. Tariq, and N. S. Dhalla (eds.), Advances in Myocardiology, Vol. 1, pp. 553–563. University Park PRess, Baltimore.
Yabe, Y., and Kashiwakura, Y. 1978. Nucleic DNA and RNA in cardiac muscle cell of experimental myocardial infarction. In: T. Kobayashi, Y. Ito, and G. Rona (eds.), Recent Advances in Studies on Cardiac Structure and Metabolism. Vol. 12: Cardiac Adaptation, pp. 415–423. University Park Press, Baltimore.
Yamada, T., Abe, H., and Yabe, Y. 1980. Myocardial energy metabolism of congestive and hypertrophic cardiomyopathy in man. J. Mol. Cell. Cardiol. 12:181.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1983 Springer Science+Business Media New York
About this chapter
Cite this chapter
Yabe, Y., Abe, H., Kashiwakura, Y. (1983). DNA Synthetic Activity of Right and Left Ventricular Biopsy Specimens in Patients with Cardiomyopathy. In: Chazov, E., Saks, V., Rona, G. (eds) Advances in Myocardiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-4441-5_13
Download citation
DOI: https://doi.org/10.1007/978-1-4757-4441-5_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-4443-9
Online ISBN: 978-1-4757-4441-5
eBook Packages: Springer Book Archive