DNA Synthetic Activity of Right and Left Ventricular Biopsy Specimens in Patients with Cardiomyopathy

  • Y. Yabe
  • H. Abe
  • Y. Kashiwakura


This investigation was designed to evaluate the difference in DNA activity between biopsy specimens obtained from right and left ventricles. Nucleic DNA in the myocardial cells of hypertrophied and congestive forms of cardiomyopathy was analyzed to investigate the relationship between cell function and clinical manifestations. Endomyocardial biopsy specimens were obtained simultaneously from right ventricular septal wall and left ventricular in-ferolateral wall by a transcatheter biotome. Measurement of DNA was based on the Feulgen reaction and dual wavelength cytophotometry. In this series, 12 patients with hypertrophic cardiomyopathy and four patients with congestive cardiomyopathy were studied. In the normal heart, the DNA value (arbitrary units) of the right ventricle was 138.6, whereas that of the left ventricle was 144.4. In the hypertrophic group, the mean DNA value in the right ventricle was 279.9, whereas that of the left ventricle was 317.5. In the congestive group, the DNA mean value in the right ventricle was 108.8, whereas that of left ventricle was 144.8. The linear relationship (r = 0.67) between right and left ventricular DNA values suggests that cellular function of one ventricle is affected by that of the other side. Higher DNA values of the left ventricle may indicate the difference in work load between the ventricles. The relationships among DNA values, LV wall thickness, LV mass, and parameters of contractility were statistically high.


Left Ventricle Cardiac Muscle Cell Myocardial Biopsy Congestive Cardiomyopathy Contractility Index 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Abe, H., and Kitamura, K. 1981. New trial of myocardial biopsy. Jpn. Circ. J. 45 (Suppl.) 22.Google Scholar
  2. 2.
    Brooksby, A. B., Jenkins, B. S., Coltart, D. J., and Webb-Peploe, M. M. 1974. Left ventricular endomyocardial biopsy. Lancet 2:1222–1225.PubMedCrossRefGoogle Scholar
  3. 3.
    Konno, S., Sekiguchi, M., and Sakakibara, S. 1971. Catheter biopsy of the heart. Radiol. Clin. North Am. 9:491–510.PubMedGoogle Scholar
  4. 4.
    Kunkel, B., Lapp, H., Kober, G., and Kaltenbach, M. 1978. Light-microscopic evaluation of myocardial biopsies. In: M. Kaltenbach, F. Loogen, and E. G. Olsen (eds.), Cardiomyopathy and Myocardial Biopsy, pp. 62–70. Springer-Verlag, Berlin, Heidelberg, New York.CrossRefGoogle Scholar
  5. 5.
    Sandritter, W., and Scomazzoni, G. 1964. DNA content (Feulgen photometry) and dry weight (interference microscopy) of normal and hypertrophic heart muscle fibres. Nature 202:100–115.PubMedCrossRefGoogle Scholar
  6. 6.
    Shirey, E. K., Hawk, W. A., Mukerji, D., and Effler, D. B. 1972. Percutaneous myocardial biopsy of the left ventricle. Experience in 198 patients. Circulation 46:112–122.PubMedCrossRefGoogle Scholar
  7. 7.
    Yabe, Y., and Abe, H. 1979. Changes in DNA and RNA synthesis of the cardiac muscle cell in patients with cardiomyopathy (myocardial biopsy). J. Mol. Cell. Cardiol. 77(Suppl. 7):68.CrossRefGoogle Scholar
  8. 8.
    Yabe, Y., and Abe, H. 1980. Changes in DNA synthesis in significantly hypertrophied human cardiac muscle. In: M. Tajuddin, P. K. Das, M. Tariq, and N. S. Dhalla (eds.), Advances in Myocardiology, Vol. 1, pp. 553–563. University Park PRess, Baltimore.Google Scholar
  9. 9.
    Yabe, Y., and Kashiwakura, Y. 1978. Nucleic DNA and RNA in cardiac muscle cell of experimental myocardial infarction. In: T. Kobayashi, Y. Ito, and G. Rona (eds.), Recent Advances in Studies on Cardiac Structure and Metabolism. Vol. 12: Cardiac Adaptation, pp. 415–423. University Park Press, Baltimore.Google Scholar
  10. 10.
    Yamada, T., Abe, H., and Yabe, Y. 1980. Myocardial energy metabolism of congestive and hypertrophic cardiomyopathy in man. J. Mol. Cell. Cardiol. 12:181.Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Y. Yabe
    • 1
  • H. Abe
    • 2
  • Y. Kashiwakura
    • 3
  1. 1.Cardiovascular Diagnostic Laboratory CenterToho University School of MedicineTokyoJapan
  2. 2.Department of RadiologyNihon University School of MedicineTokyo, 173Japan
  3. 3.Department of CardiologyUrawa City HospitalUrawaJapan

Personalised recommendations