DNA Synthesis and Mitotic Activity in Adult Atrial Cardiocytes in Culture

  • M. Cantin
  • M. Ballak
  • J. Beuzeron-Mangina
  • C. Tautu

Abstract

Trypsin-dissociated adult rat atrial cardiocytes were exposed to [3H]thymidine for sequential 24-hr periods from day 3 to day 13 of culture. Following preparation for ultrastructural autoradiography, 1000 cells (at each daily interval) were examined with an electron microscope. Maximal incorporation occurred on day 5 when 63% of the cells were labeled. Small secondary peaks of incorporation occurred on days 8 and 11 following previous exposure to fresh serum. Mitotic activity never exceeded 0.5% of all cardiocytes examined. DNA synthesis and mitoses occurred only in immature cardiocytes characterized by subsarcolemmal filaments and Z-bands with or without specific granules; more mature cells were never labeled.

Keywords

Mitotic Activity Rough Endoplasmic Reticulum Label Nucleus Specific Granule Clinical Research Institute 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • M. Cantin
    • 1
    • 2
  • M. Ballak
    • 1
    • 2
  • J. Beuzeron-Mangina
    • 1
    • 2
  • C. Tautu
    • 1
    • 2
  1. 1.Laboratory of PathobiologyClinical Research Institute of MontrealMontrealCanada
  2. 2.Department of PathologyUniversity of Montreal and Hôtel-Dieu of MontrealMontrealCanada

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