Baculovirus Genomics: A Resource for Biocontrol

  • Vernon K. Ward
  • Katherine M. B. Sneddon
  • Otto Hyink
  • James Kalmakoff


Baculoviruses are the most studied group of invertebrate viruses and are unique among the invertebrate viruses in how important they have become to many other fields of research. This has largely come about through their development as one of the premiere eukaryotic protein expression systems available today (Kost and Condreay, 1999). Despite this wide use of baculoviruses, much oftheir biology remains to be deciphered. By combining the knowledge being derived from baculovirus genome analysis with studies into the biology of these viruses and their hosts, many advances are being made in our understanding of how these viruses work. Baculovirus genome analysis is also providing the basis for elucidating the roles of many baculovirus genes. This is giving new leads into how baculoviruses can be better utilised or improved, and supplying genes that have the potential to be used effectively in other pest control strategies.


Chitinase Gene Nuclear Polyhedrosis Virus Peritrophic Membrane Prothoracic Gland Peritrophic Matrix 
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  1. Afonso, C. L., Tulman, E. R., Lu, Z., Balinsky, C. A., Moser, B. A., Becnel, J. J., Rock, D. L., and Kutish, G. F., 2001, Genome sequence of a baculovirus pathogenic for Culex nigripalpus,, J Virol. 75: 11157–11165.PubMedCrossRefGoogle Scholar
  2. Ahrens, C. H., Russell, R. L. Q., Funk, C. J., Evans, J. T., Harwood, S. H., and Rohrmann, G. F., 1997, The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome, Virol. 229: 381–399.CrossRefGoogle Scholar
  3. Ayres, M. D., Howard, S. C., Kuzio, J., Lopez-Ferber, M., and Possee, R. D., 1994, The complete DNA sequence of Autographa californica nuclear polyhedrosis virus, Virol. 202: 586–605.CrossRefGoogle Scholar
  4. Beidler, D. R., Tewari, M., Friesen, P. D., Poirer, G., and Dixit, V. M., 1995, The baculovirus P35 protein inhibits Fas-and Tumour Necrosis Factor-induced apoptosis, J Biol. Chem. 270: 16526–16528.PubMedCrossRefGoogle Scholar
  5. Bertin, J., Mendrysa, S. M., LaCount, D. J., Gaur, S., Krebs, J. F., Armstrong, R. C., Tomaselli, K. J., and Friesen, P. D., 1996, Apoptotic suppression by baculovirus P35 involves cleavage by and inhibition of a virus-induced CED-3/ICE-like protease, J. Virol. 70: 6251–6259.PubMedGoogle Scholar
  6. Bischoff, D. S., and Slavicek, J. M., 1997, Molecular analysis of an enhancin gene in the Lymantria dispar nuclear polyhedrosis virus, J. Virol. 71: 8133–8140.PubMedGoogle Scholar
  7. Brandt, C. R., Adang, M. J., and Spence, K. D., 1978, The peritrophic membrane: ultrastructural analysis and function as a mechanical barrier to microbial infection in Orgyia pseudotsugata, J Invertebr. Pathol. 32: 1224.CrossRefGoogle Scholar
  8. Bump, N. J., Hackett, M., Hugunin, M., Seshagiri, S., Brady, K., Chen, R, Ferenz, C., Franklin, S., Ghayur, T., Li, P., Licari, P., Mankovich, J., Shi, L., Greenberg, A. H., Miller, L. K., and Wong, W. W., 1995, Inhibition of ICE family proteases by baculovirus antiapoptotic protein P35, Science 269: 1885–1888.PubMedCrossRefGoogle Scholar
  9. Burand, J. P., and Park, E. J., 1992, Effect of nuclear polyhedrosis virus infection on the development and pupation of gypsy moth larvae, J. Invertebr. Pathol. 60: 171–175.CrossRefGoogle Scholar
  10. Caradoc-Davies, K. M. B., Graves, S., O’Reilly, D. R., Evans, O. P., and Ward, V. K., 2001, Identification and in vivo characterization of the Epiphyas postvittana nucleopolyhedrovirus ecdysteroid UDP-glucosyltransferase, Virus Genes 22: 255–264.PubMedCrossRefGoogle Scholar
  11. Chejanovsky, N., and Gershberg, E., 1995, The wild-type Autographa californica nuclear polyhedrosis virus induces apoptosis of Spodoptera littoralis cells, Virol. 209: 519–525.CrossRefGoogle Scholar
  12. Chen, X., W.F., L, Tarchini, R., Sun, X., Sandbrink, H., Wang, H., Peters, S., Zuidema, D., Lankhorst, R. K., Vlak, J. M., and Hu, Z., 2001, The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome, J. Gen. Virol. 82: 241–257.Google Scholar
  13. Chemin, L., Brandis, A., Ismailov, Z., and Chet, I., 1996, Pyrrolnitrin production by an Enterobacter agglomerans strain with a broad spectrum of antagonistic activity towards fungal and bacterial phytopathogens, Cure Microbiol. 32: 208–212.CrossRefGoogle Scholar
  14. Clarke, E. E., Tristem, M., Cory, J. S., and Oreilly, D. R., 1996, Characterization of the ecdysteroid UDP-glucosyltransferase gene from Mamestra brassicae nucleopolyhedrovirus, J Gen. Viro!. 77: 2865–2871.CrossRefGoogle Scholar
  15. Clem, R. J., Fechheimer, M., and Miller, L. K., 1991, Prevention of apoptosis by a baculovirus gene during infection of insect cells, Science 254: 1388–1390.PubMedCrossRefGoogle Scholar
  16. Clem, R. J., and Miller, L. K., 1993, Apoptosis reduces both the in vitro replication and the in vivo infectivity of a baculovirus, J Virol. 67: 3730–3738.PubMedGoogle Scholar
  17. Clem, R. J., and Miller, L. K., 1994, Control of programmed cell death by the baculovirus genes p35 and iap, Molec. Cell Biol. 14: 5212–5222.PubMedGoogle Scholar
  18. Crook, N. E., Clem, R. J., and Miller, L. K., 1993, An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif, J Virol. 67: 2168–2174.PubMedGoogle Scholar
  19. Davison, J., 1988, Plant beneficial bacteria, Bio-Technol6:282–286.Google Scholar
  20. Derksen, A. C. G., and Granados, R. R., 1988, Alteration of a lepidopteran peritrophic membrane by baculoviruses and enhancement of viral infectivity, Virol. 167: 242–250.CrossRefGoogle Scholar
  21. Ding, X. F., Gopalakrishnan, B., Johnson, L. B., White, F. F., Wang, X. R., Morgan, T. D., Kramer, K. J., and Muthukrishnan, S., 1998, Insect resistance of transgenic tobacco expressing an insect chitinase gene, Transgenic Res. 7:77–84.Google Scholar
  22. Downing, K. J., and Thomson, J. A., 2000, Introduction of the Serratia marcescens chiA gene into an endophytic Pseudomonas fuorescens for the biocontrol of phytopathogenic fungi, Can. J. Microbiol. 46: 363–369.PubMedGoogle Scholar
  23. Du, Q., Lehavi, D., Faktor, O., Qi, Y., and Chejanovsky, N., 1999, Isolation of an apoptosis suppressor gene of the Spodoptera littoralis nucleopolyhedrovirus, J. Virol. 73: 1278–1285.PubMedGoogle Scholar
  24. Eddins, M. J., Lemongello, D., Friesen, R D., and Fisher, A. J., 2002, Crystallization and low-resolution structure of an effector-caspase/P35 complex: similarities and differences to an initiator-caspase/P35 complex, Acta Crystallogr. D. Biol. Crystallogr. 58: 299–302.PubMedCrossRefGoogle Scholar
  25. Evans, O. P., and O’Reilly, D. R., 1998, Purification and kinetic analysis of a baculovirus ecdysteroid UDPglycosyltransferase, Biochem. J. 330: 1265–1270.PubMedGoogle Scholar
  26. Evans, O. P., and O’Reilly, D. R., 1999, Expression and structural characterization of a baculovirus ecdysteroid UDP-glucosyltransferase, J. Gen. Viro!. 80: 485–492.Google Scholar
  27. Gomi, S., Maj ima, K., and Maeda, S., 1999, Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus, J. Gen. Viro!. 80: 1323–1337.Google Scholar
  28. Gopalakrishnan, B., Muthukrishnan, S., and Kramer, K. J., 1995, Baculovirus-mediated expression of a Manduca sexta chitinase gene–properties of the recombinant protein, Insect Biochem. Molec. Biol. 25: 255–265.CrossRefGoogle Scholar
  29. Graves, S., Markwick, N. R, Vickers, J. M., Christeller, J. T., and Ward, V. K., 1998, 4th International Workshop onMicrobial Control of Soil Dwelling Pests, Lincoln. Google Scholar
  30. Greenspan-Gallo, L., Corsaro, B. G., Hughes, R. R., and Granados, R. R., 1991, In vivo enhancement of baculovirus infection by the viral enhancing factor of a granulosis virus of the cabbage looper, Trichoplusia ni (Lepidoptera: Noctuidae), J. Invertebr. Pathol. 58: 203–210.CrossRefGoogle Scholar
  31. Gross, C. H., Wolgamot, G. M., Russell, R. L., Pearson, M. N., and Rohrmann, G. F., 1993, A 37-kilodalton glycoprotein from a baculovirus of Orgyia pseudotsugata is localized to cytoplasmic inclusion bodies, J Virol. 67: 469475.Google Scholar
  32. Hashimoto, Y., Corsaro, B. G., and Granados, R. R., 1991, Location and nucleotide sequence of the gene encoding the viral enhancing factor of the Trichoplusia ni granulosis virus, J. Gen. Viro!. 72: 2645–2651.CrossRefGoogle Scholar
  33. Hashimoto, Y., Hayakawa, T., Ueno, Y., Fujita, T., Sano, Y., and Matsumoto, T., 2000, Sequence analysis of the Plutellaxylostella granulovirus genome, Virol. 275: 358–372.CrossRefGoogle Scholar
  34. Hawtin, R. E., Arnold, K., Ayres, M. D., Zanotto, P. M. D., Howard, S. C., Gooday, G. W., Chappell, L. H., Kitts, P. A., King, L. A., and Possee, R. D., 1995, Identification and preliminary characterization of a chitinase gene in the Autographa californica Nuclear Polyhedrosis Virus genome, Virol. 212: 673–685.CrossRefGoogle Scholar
  35. Hawtin, R. E., Zarkowska, T., Arnold, K., Thomas, C. J., Gooday, G. W., King, L. A., Kuzio, J. A., and Possee, R. D., 1997, Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes, Virol. 238: 243–253.CrossRefGoogle Scholar
  36. Hay, B. A., Wolff, T., and Rubin, G. M., 1994, Expression of baculovirus P35 prevents cell death in Drosophila, Development 120: 2121–2129.PubMedGoogle Scholar
  37. Hayakawa, T., Ko, R., Okano, K., Seong, S. I., Goto, C., and Maeda, S., 1999, Sequence analysis of the Xestia cnigrum granulovirus genome, Viro!. 262: 277–297.CrossRefGoogle Scholar
  38. Hayakawa, T., Rohrmann, G. F., and Hashimoto, Y., 2000, Patterns of genome organization and content in lepidopteran baculoviruses, Virol. 278: 1–12.CrossRefGoogle Scholar
  39. Hayakawa, T., Xu, J., and Hukuhara, T., 1996, Cloning and sequencing of the gene for an enhancing factor from Pseudaletia separata entomopoxvirus, Gene 177:269–270.Google Scholar
  40. Heldens, J. G., Liu, Y., Zuidema, D., Goldbach, R. W., and Vlak, J. M., 1998, A highly conserved genomic region in baculoviruses: sequence analysis of an 11.3 kbp DNA fragment (46.5–55.1 m.u.) of the Spodoptera exigua multicapsid nucleopolyhedrovirus, Virus Res. 55: 187–198.PubMedCrossRefGoogle Scholar
  41. Herniou, E. A., Luque, T., Chen, X., Vlak, J. M., Winstanley, D., Cory, J. S., and O’Reilly, D., 2001, Use of whole genome sequence data to infer Baculovirus phylogeny, J. Viral. 75: 8117–8126.CrossRefGoogle Scholar
  42. Hershberger, P. A., Dickson, J. A., and Friesen, P. D., 1992, Site-specific mutagenesis of the 35-kilodalton protein gene encoded by Autographa californica nuclear polyhedrosis virus: cell line-specific effects on virus replication, J. Viral. 66: 5525–5533.Google Scholar
  43. Holcik, M., and Korneluk, R. G., 2001, XIAP, the guardian angel, Nat. Rev. Mol. Cell Biol. 2: 550–556.PubMedCrossRefGoogle Scholar
  44. Hozak, R. R., Manji, G.A., and Friesen, P. D., 2000, The BIR motifs mediate dominant interference and oligomerisation of inhibitor of apoptosis Op-IAP, Molec. Cell Biol. 20: 1877–1885.PubMedCrossRefGoogle Scholar
  45. Hu, Z. H., Arif, B. M., Jin, F., Martens, J. W. M., Chen, X. W., Sun, J. S., Zuidema, D., Goldbach, R. W., and Vlak, J. M., 1998, Distinct gene arrangement in the Buzura suppressaria single-nucleocapsid nucleopolyhedrovirus genome, J. Gen. Viral. 79: 2841–2851.Google Scholar
  46. Hukuhara, T., Hayakawa, T., and Wijonarko, A., 1999, Increased baculovirus susceptibility of armyworm larvae feeding on transgenic rice plants expressing an entomopoxvirus gene, Nature Biotech. 17:1122–1124.Google Scholar
  47. Hukuhara, T., Hayakawa, T., and Wijonarko, A., 2001, A bacterially produced virus enhancing factor from an entomopoxvirus enhances nucleopolyhedrovirus infection in armyworm larvae, Jlnvertebz Pathol. 78: 2530.Google Scholar
  48. Hukuhara, T., and Zhu, Y., 1989, Enhancement of the in vitro infectivity of a nuclear polyhedrosis virus by a factor in the capsule of a granulosis virus, J Invertebr. Pathol. 54: 71–78.CrossRefGoogle Scholar
  49. Hyink, O., Dellow, R. A., Olsen, M. J., Caradoc-Davies, K. M. B., Drake, K., Herniou, E. A., Cory, J. S., O’Reilly, D. R., and Ward, V. K., 2002, Whole genome analysis of the Epiphyas postvittana nucleopolyhedrovirus, J Gen. Viral. 83: 959–973.Google Scholar
  50. Hyink, O., Graves, S., Fairbairn, F. M., and Ward, V. K., 1998, Mapping and polyhedrin gene analysis of the Epiphyas postvittana nucleopolyhedrovirus genome, J Gen. Viral. 79: 2853–2862.Google Scholar
  51. Ijkel, W. F. J., van Strien, E. A., Heldens, J. G. M., Broer, R., Zuidema, D., Goldblach, R. W., and Vlak, J. M., 1999, Sequence and organisation of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome, J. Gen. Viral. 80: 3289–3304.Google Scholar
  52. Kost, T. A., and Condreay, J. P., 1999, Recombinant baculoviruses as expression vectors for insect and mammalian cells, Curr. Opin. Biotech. 10: 428–433.PubMedCrossRefGoogle Scholar
  53. Komma, K., and Hukuhara, T., 1994, Fusion characteristics of a nuclear polyhedrosis virus in cultured cells: Time course and effect of a synergistic factor and pH, J. Invertebr. Pathol. 63: 63–67.CrossRefGoogle Scholar
  54. Kumar, S., and Miller, L. K., 1987, Effects of serial passage of Autographa californica nuclear polyhedrosis virus in cell culture, Virus Res. 7: 335–349.PubMedCrossRefGoogle Scholar
  55. Kuzio, J., Pearson, M. N., Harwood, S. H., Funk, C. J., Evans, J. T., Slavicek, J. M., and Rohrmann, G. F., 1999, Sequence and analysis of the genome of a baculovirus pathogenic for Lymantria dispar, Viral. 253: 17–34.Google Scholar
  56. LaCount, D. J., Hanson, S. F., Schneider, C. L., and Friesen, P. D., 2000, Caspase inhibitor P35 and inhibitor of apoptosis Op-IAP block in vivo proteolytic activation of an effector caspase at different steps, J.Biol. Chem. 275: 15657–15664.PubMedCrossRefGoogle Scholar
  57. Lafont, R., and Connat, J.-L., 1989, Pathways of ecdysone metabolism, in: Ecdysone, J. Koolman, ed., Verlag, Stuttgart, pp. 167–173.Google Scholar
  58. Lee, J. C., Chen, H. H., and Chao, Y. C., 1998, Persistent baculovirus infection results from deletion of the apoptotic suppressor gene p35, J. Viral. 72: 9157–9165.Google Scholar
  59. Lepore, L. S., Roelvink, P. R., and Granados, R. R., 1996, Enhancin, the granulosis virus protein that facilitates nucleopolyhedrovirus (NPV) infections, is a metalloprotease, J. Invertebr. Pathol. 68: 131–140.PubMedCrossRefGoogle Scholar
  60. Luque, T., Finch, R., Crook, N., O’Reilly, D. R., and Winstanley, D., 2001, The complete sequence of the Cydia pomonella granulovirus genome, J. Gen. Viral. 82: 2531–2547.Google Scholar
  61. Maguire, T., Harrison, P., Hyink, O., Kalmakoff, J., and Ward, V. K., 2000, The inhibitors of apoptosis of Epiphyas postvittana nucleopolyhedrovirus, J. Gen. Viral. 81: 2803–2811.Google Scholar
  62. Mastrangelo, A. J., and Betenbaugh, M. J., 1998, Overcoming apoptosis: new methods for improving protein-expression systems, Trends Biotechnol 16: 88–95.PubMedCrossRefGoogle Scholar
  63. Miller, L. K., 1997, The Baculoviruses, in: The Viruses, H. Fraenkel-Conrat and R. R. Wagner, eds., Plenum Press, New York, 447 pp.Google Scholar
  64. Morris, O. N., 1976, A 2-year study of the efficacy of Bacillus thuringiensis-chitinase combinations in spruce budworm (Choristoneurafumiferana) control, Can. Entamai. 108: 3225–3233.Google Scholar
  65. Muzzarelli, R. A. A., 1999, Native, industrial and fossil chitins, in: Chitin and Chitinases, Vol. 87, P. Jolies and R. A. A. Muzzarelli, eds., Birkhauser Verlag, Basel, pp. 1–8.Google Scholar
  66. Nijhout, H. F., 1994, Insect Hormones,Princeton University Press.Google Scholar
  67. O’Brien, V., 1998, Viruses and apoptosis, J. Gen. Virol. 79: 1833–1845.PubMedGoogle Scholar
  68. Oppenheim, A. B., and Chet, I., 1992, Cloned chitinases in fungal plant-pathogen control strategies, Trends Biotechnol. 10: 392–394.Google Scholar
  69. O’Reilly, D. R., Brown, M. R., and Miller, L. K., 1992, Alteration of ecdysteroid metabolism due to baculovirus infection of the fall armyworm Spodoptera frugiperda: host ecdysteroids are conjugated with galatose, Insect Biochem. Molec. Biol. 22: 313–320.CrossRefGoogle Scholar
  70. O’Reilly, D. R., and Miller, L. K., 1989, A baculovirus blocks insect molting by producing ecdysteroid UDPglucosyl transferase, Science 245: 1110–1112.PubMedCrossRefGoogle Scholar
  71. O’Reilly, D. R., and Miller, L. K., 1991, Improvement of a baculovirus pesticide by deletion of the egt gene, Bio/ technology 9: 1086–1089.CrossRefGoogle Scholar
  72. Pang, Y., Yu, J., Wang, L., Hu, X., Bao, W., Li, G., Chen, C., Han, H., Hu, S., and Yang, H., 2001, Sequence analysis of the Spodoptera litura multicapsid nucleopolyhedrovirus genome, Virol. 287: 391–404.CrossRefGoogle Scholar
  73. Park, E. J., Burand, J. P., and Yin, C.-M., 1993, The effect of baculovirus infection on ecdysteroid titer in gypsy moth larvae (Lymantria dispar), J. Insect Physiol. 39: 791–796.CrossRefGoogle Scholar
  74. Park, E. J., Yin, C.-M., and Burand, J. P., 1996, Baculovirus replication alters hormone-regulated host development, J Gen. Virol. 77: 547–554.Google Scholar
  75. Phanis, C. G., Miller, D. P., Cassar, S. C., Tristem, M., Thiem, S. M., and O’Reilly, D. R., 1999, Identification and expression of two baculovirus gp37 genes, J. Gen. Virol. 80: 1823–1831.Google Scholar
  76. Popham, H. J., Bischoff, D. S., and Slavicek, J. M., 2001, Both Lymantria dispar nucleopolyhedrovirus enhancin genes contribute to viral potency, J. Virol. 75: 8639–8648.PubMedCrossRefGoogle Scholar
  77. Roelvink, P. W., Corsaro, B. G., and Granados, R. R., 1995, Characterization of the Helicoverpa armigera and Pseudaletia unipuncta granulovirus enhancin genes, J. Gen. Virol. 76: 2693–2705.Google Scholar
  78. Roy, N., Deveraux, Q. L., Takahashi, R., Salvesen, G. S., and Reed, J. C., 1997, The c-IAP-1 and c-IAP-2 proteins are direct inhibitors of specific caspases, EMBO J. 16: 6914–6925.PubMedCrossRefGoogle Scholar
  79. Sadler, T. J., Glare, T. R., Ward, V. K., and Kalmakoff, J., 2000, Physical and genetic map of the Wiseana nucleopolyhedrovirus genome, J. Gen. Virol. 81: 1127–1133.Google Scholar
  80. Sakurai, S., and Williams, C. M., 1989, Short-loop negative and positive feedback on ecdysone secretion by prothoracic gland in the tobacco homworm, Manduca sexto, Gen. Comp. Endocrino!. 75: 204–216.CrossRefGoogle Scholar
  81. Seshagiri, S., and Miller, L. K., 1997, Baculovirus inhibitors of apoptosis (IAPs) block activation ofSf-caspase-1, Proc. Natl. Acad Sci. USA 94: 13606–13611.PubMedCrossRefGoogle Scholar
  82. Shapiro, M., Preisler, H. K., and Robertson, J. L., 1987, Enhancement of baculovirus activity on Gypsy moth (Lepidoptera, Lymantriidae) by chitinase, J. Econ. Entomol. 80: 1113–1116.Google Scholar
  83. Sitrit, Y., Barak, Z., Kapulnik, Y., Oppenheim, A. B., and Chet, l., 1993, Expression ofSerratia marcescens chitinase gene in Rhizobium meliloti during symbiosis on alfalfa roots, Mol. Plant-Microbe Interact. 6: 293–298.CrossRefGoogle Scholar
  84. Slavicek, J. M., Popham, H. J. R., and Riegel, C. I., 1999, Deletion of the Lymantria dispar multicapsid nucleopolyhedrovirus ecdysteroid UDP-glucosyl transferase gene enhances viral killing speed in the last instar of the gypsy moth, Biol. Cont. 16: 91–103.CrossRefGoogle Scholar
  85. Smimoff, W. A., 1974, Three years of aerial field experiments with Bacillus thuringiensis plus chitinase formulation against the spruce budworm, J Invertebr. Pathol. 24: 344–348.Google Scholar
  86. Sugimoto, A., Friesen, P. D., and Rothman, J. H., 1994, Baculovirus p35 prevents developmentally programmed cell death and rescues a ced-9 mutant in the nematode Caenorhabditis elegans, EMBO J. 13: 2023–2028.PubMedGoogle Scholar
  87. Tanada, Y., 1956, Some factors affecting the susceptibility of the armyworm to virus infections, J. Econ. Entomol. 49: 52–57.Google Scholar
  88. Tanada, Y., and Hukuhara, T., 1968, A non-synergistic strain of a granulosis virus of the armyworm, Pseudaletia unipuncta, J. Invertebr. Pathol. 12: 263–268.Google Scholar
  89. Tanada, Y., and Hukuhara, T., 1971, Enhanced infection of a nuclear polyhedrosis virus in larvae of the armywormGoogle Scholar
  90. Pseudoletia unipuncta, by a factor in the capsule of a granulosis virus, J Invertebr. Pathol. 17:116–126. Thomas, C. J., Gooday, G. W., King, L. A., and Possee, R. D., 2000, Mutagenesis of the active site coding region of the Autographa californica nucleopolyhedrovirus chiA gene, J. Gen. Virol. 81: 1403–1411.Google Scholar
  91. Uren, A. G., and Vaux, D. L., 1997, Viral inhibitors of apoptosis, Vitamin. Hormones 53: 175–193.CrossRefGoogle Scholar
  92. van Regenmortel, M. H. V., Fauquet, C. M., Bishop, D. H. L., Carstens, E. B., Estes, M. K., Lemon, S. M., ManiloffGoogle Scholar
  93. J., Mayo, M. A., McGeoch, D. J., Pringle, C. R., and Widmer, R. B., 2000, Viru; Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses, 7 ed. Academic Press, San Diego, USA. Vaux, D. L., and Korsmeyer, S. J., 1999, Cell death in development, Ce!! 96: 245–254.Google Scholar
  94. Verhagen, A. M., Coulson, E. J., and Vaux, D. L., 2001, Inhibitor of apoptosis proteins and their relatives: IAPs and other BIRPs, Genome Bio12:REVIEWS3009.Google Scholar
  95. Vialard, J. E., Yuen, L., and Richardson, C. D., 1990, Identification and characterization of a baculovirus occlusion body glycoprotein which resembles spheroidin, an entomopoxvirus protein, J. Virol. 64: 5804–5811.PubMedGoogle Scholar
  96. Wang, P., and Granados, R. R., 1997, An intestinal mucin is the target substrate for a baculovirus enhancin, Proc. Natl. Acad. Sci. USA 94: 6977–6982.CrossRefGoogle Scholar
  97. Wang, P., and Granados, R. R., 1998, Observations on the presence of the peritrophic membrane in larval Trichoplusia ni and its role in limiting baculovirus infection, J. Invertebr. Pathol. 72: 57–62.PubMedCrossRefGoogle Scholar
  98. Wang, P., Hammer, D. A., and Granados, R. R., 1994, Interaction of Trichoplusia ni granulosis virus-encoded enhancin with the midgut epithelium and peritrophic membrane of four lepidopteran insects, J. Gen. Viro!. 75: 1961–1967.CrossRefGoogle Scholar
  99. Wang, P., Hammer, D. A., and Granados, R. R., 1997, Binding and fusion of Autographa californica nucleopolyhedrovirus to cultured insect cells, J. Gen. Virol. 78: 3081–3089.PubMedGoogle Scholar
  100. Wiwat, C., Thaithanun, S., Pantuwatana, S., and Bhumiratana, A., 2000, Toxicity of chitinase-producing Bacillus thuringiensis ssp kurstaki HD-1 (G) toward Plutella xylostella, J Invertebr. Pathol. 76: 270–277.PubMedCrossRefGoogle Scholar
  101. Xu, J., and Hukuhara, T., 1992, Enhanced infection of a nuclear polyhedrosis virus in larvae of the armyworm, Pseudoletia separata, by a factor in the spheroids of an entomopoxvirus, J Invertebr. Pathol. 60: 259–264.CrossRefGoogle Scholar
  102. Xu, J., and Hukuhara, T., 1994, Biochemical properties of an enhancing factor of an entomopoxvirus, J Invertebr. athol. 63: 14–18.Google Scholar
  103. Yang, Y., Fang, S., Jensen, J. P., Weissman, A. M., and Ashwell, J. D., 2000, Ubiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli, Science 288: 874–877.PubMedCrossRefGoogle Scholar
  104. Zanotto, P. M., Kessing, B. D., and Maruniak, J. E., 1993, Phylogenetic interrelationships among baculoviruses: Evolutionary rates and host associations, J. Invertebr. Pathol. 62: 147–164.PubMedCrossRefGoogle Scholar
  105. Zoog, S. J., Bertin, J., and Friesen, P. D., 1999, Caspase inhibition by baculovirus P35 requires interaction between the reactive site loop and the beta-sheet core, J Chem. 274: 25995–26002.Google Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Vernon K. Ward
    • 1
  • Katherine M. B. Sneddon
    • 1
  • Otto Hyink
    • 1
  • James Kalmakoff
    • 1
  1. 1.Department of Microbiology, School of Medical SciencesUniversity of OtagoDunedinNew Zealand

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