Measurements of Basilar Membrane Tuning and Distortion with Laser Doppler Velocimetry

  • A. L. Nuttall
  • D. F. Dolan
  • G. Avinash
Part of the Lecture Notes in Biomathematics book series (LNBM, volume 87)


A method is described for the measurement of basilar membrane (BM) vibration. It makes use of commercially made laser Doppler velocimetry (LDV) instrumentation which is adapted to a compound microscope. This allows visualization reflective objects placed on the EM and the focusing of the laser beam onto the reflective object (a glass microbead). We show examples of frequency tuning curves and displacement input/output intensity functions obtained with the technique and demonstrate that cubic distortion of both the local and propagated kind are present in mechanical responses of the membrane.


Hair Cell Basilar Membrane Compound Action Potential Distortion Product Osseous Spiral Lamina 
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Literature Cited

  1. Bekesy, G. von. (1960): Experiments in Hearing. (McGraw Hill. NY).Google Scholar
  2. Brown MC, Nuttall AL. Masta RI. (1983b): lntracellularrecordings from cochlear inner hair cells: Effects of stimulation of the crossed olivocochlear efferents. Science 222:69–72.Google Scholar
  3. Brown MC. Smith DI. Nuttall AL. (1983a): Anesthesia and surgical trauma: Their influence on the guinea pig compound action potential. Hearing Res. 10:345–358.Google Scholar
  4. Brown. MC and Nuttall, AL. (1984): Efferent control of cochlear inner hair cell responses in the guinea pig. J. Physiol. 354:625–646.Google Scholar
  5. Dallos P. (1985): Respcnse characteristics of mammalian cochlear hair cells. J Neurosci 5: 1591– 1608.Google Scholar
  6. Durrani TS and Greated CA. (1977): Laser Systems in Flow Measurement. Plenum Press. New York.Google Scholar
  7. Evans EF. (1979): Single unit studies of the mammalian auditory nerves. In: Auditory Investigations: The Scientific and Technological Basis. Ed. H.A. Beagley. Oxford Univ. Press.Google Scholar
  8. Geisler CD. Rhode WS. Kennedy DT. (1974): Responses to tonal stimuli of single auditory nerve fibers and their relation to basilar membrane motion in the squirrel monkey. J Neurophysiol 37:1156–1172.Google Scholar
  9. Goodman DA. Smith RL. Chamberlain SC. (1982): Intracellular and extracellular responses in the organ of Corti of the gerbil. Hearing Res. 7: 161–179.Google Scholar
  10. Johnstone BM. Boyle AJF. (1967): Basilar membrane vibration examined with the Mossbauer technique. Science 158:389–390.Google Scholar
  11. Khanna SM and Koester CJ. (1989): Optical sectioning characteristics of the heterodyne interferometer. Acta Otolaryngol (Stockh) SuppI467:61–68.Google Scholar
  12. Khanna SM. Johnson GW. Jacobs J. (1986): Homodyne interferometer for basilar membrane measurements. II. Hardware and techniques. Hearing Res. 23: 27–36.Google Scholar
  13. Khanna SM. Leonard DGB. (1982): B asilar membrane tuning in the cat cochlea. Science 215: 305– 306.Google Scholar
  14. Khanna SM. Willemin JF. and Ulfendahl M. (1989): Measurements of optical reflectivity in cells of the inner ear. Acta Otolaryngol (Stockh) SuppI467:69–75.Google Scholar
  15. Khanna SM. (1986): Homodyne interferometer for basilar membrane measurements. Hearing Res 23: 9–26.Google Scholar
  16. Kiang NYS. Watanabe T. Thomas EC. Clark LF. (1965): Discharge patterns of single fibers in the eat’s auditory nerve. Research Monograph #35 (MIT. Cambridge).Google Scholar
  17. Kliauga p. Khanna SM. (1983): Dose rate to the inner ear during MOssbauer expeliments. Phys Med Bio. 28:359–366.Google Scholar
  18. Kohlloffel LUE. (1972a): A study of basilar membrane vibrations. I. Fuzziness-detection: A new method for the analysis of microvibrations with laser light. Acustica 27:49–65.Google Scholar
  19. Kohlloffel LUE. (1972b): A study of basilar membrane vibrations.II. The vibratory amplitude and phase pattern along the basilar membrane (post–mortem). Acustica 27. 66–8.Google Scholar
  20. Kohlloffel LUE. (1972c): A study of basilar membrane vibrations. lli. The basilar membrane frequency response curve in the living guinea pig. Acustica 27: 82–89.Google Scholar
  21. LePage EL. (1987): The application of a capacitative probe technique for direct observation of electromechanical processes in the guinea pig cochlea. J Acoust Soc Am 82:126–38.Google Scholar
  22. LePage EL. (1988): Fiber optic lever reveals sound-produced contractile component of basilar membrane motion. Abstracts Association for Research in Otolaryngology. Clearwater Beach. FL.Google Scholar
  23. Nuttall AL. Dolan DF. Gopal A. (1989): Laser Doppler vibrometer measurements of basilar membrane motion in the guinea pig. Society for Neuroscience meeting. Phoenix. AZ.Google Scholar
  24. Patuzzi RB. Sellick P. (1983): A comparison between basilar membrane and inner hair cells receptor potential input–output functions in the guinea pig cochlea. J Acoust Soc Am 74:1731–1741.Google Scholar
  25. Rhode WS. Robles L. (1974): Evidence from Mossbauerexperiments fornonlinear vibrations in the cochlea. J Acoust Soc Am 55:588–596.Google Scholar
  26. Rhode WS. (1971): Observations of the vibration of the basilar membrane in squirrel monkeys using the Mossbauer technique. J Acoust Soc Am 49: 1218.Google Scholar
  27. Russell LJ. Sellick PM. (1978): Intracellular studies of hair cells in the mammalian cochlea. J Physiol 284: 261–290.Google Scholar
  28. Russell UJ. Sellick PM. (1977): Tuning properties of cochlear hair cells. Nature 267:858–860.Google Scholar
  29. Sachs MB. Abbas PJ. (1974): Rate versus level functions for auditory nerve fibers in cats: Tone burst stimuli. J Acoust. Soc. Am. 56:1835–1847.Google Scholar
  30. Sellick PM. Patuzzi R. Johnstone BM. (1982): Measurement of basilar membrane motion in the guinea pig using the Mossbauer technique. J Acoust Soc Am 72: 131–141.Google Scholar
  31. Sellick PM. Patuzzi R. Johnstone BM. (1983b): Comparison between the tuning properties of inner hair cells and basilar membrane motion. Hearing Res. 10: 93–100.Google Scholar
  32. Sellick PM. Yates GK. Paluzzi R. (1983a): The influence of Mossbauer source size and position on phase and amplitude measurements of the guinea pig basilar membrane. Hearing Res. 10: I 01– 108.Google Scholar
  33. Shore, SE and Nuttall. AL.: (1985): The effects of cochlear hypothermia on compound action potential tuning. J. Acous!. Soc. Am. 77:590–598.Google Scholar
  34. Willemin J-F. Diindliker R. Khanna SM. (1988): Heterodyne interferometer for SUbmicroscopic vibration measurements in the inner ear. J Acoust Soc Am 83: 787–795.Google Scholar
  35. Wilson JP. Johnstone JR. (1975): Basilar membrane and middle-ear vibration in guinea pig measured by capacitive probe. J Acoust Soc Am 57: 705–723.Google Scholar
  36. Wilson JP. (1973): A sub–miniature capacitative probe for vibration measurements of the basilar membrane. J Sound Vib 30: 483–493.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • A. L. Nuttall
    • 1
  • D. F. Dolan
    • 1
  • G. Avinash
    • 1
  1. 1.Kresge Hearing Research InstituteUniversity of Michigan Medical SchoolAnn ArborUSA

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