Structural Abnormalities in Inner Hair Cells Following Kanamycin-Induced Outer Hair Cell Loss
The aminoglycoside antibiotic kanamycin has been widely used in studies of the comparative function of inner (IHC) and outer (OHC) hair cells of the mammalian cochlea. Administration of this drug has been used to produce dose-dependent selective OHC loss in certain regions of the cochlea whilst leaving IHCs apparently intact as judged by Nomarski light microscopy (LM). In guinea pig (Harrison and Evans. 1977), and also in cat (see e.g., Kiang et al. 1986),loss of frequency selectivity and elevated thresholds have been reported in afferent nerve fibres of IHCs from regions of total OHC loss, whilst in chinchilla, elevation of threshold without loss of frequency selectivity has been found (Dallos and Harris, 1978). These results are considered consistent with other data which suggest that whilst the mcs are the primary sensory receptors of the mammalian cochlea, the OHCs are involved in some way in the active mechanical enhancement of the IHC response (see e.g., review by Patuzzi and Robertson, 1988).
KeywordsHair Cell Apical Region Outer Hair Cell Afferent Terminal Hair Cell Loss
Unable to display preview. Download preview PDF.
- Dallos, P. and Harris, D. (1978) Properties of auditory nerve responses in absence of outer hair cells. J. Neurophysio. 41, 365 - 383.Google Scholar
- Furness. D.N. and Hackney, C.M. (1986) Morphological changes to the stereociliary bundles in the guinea pig cochlea after kanamycin treatment. Brit. J. Audio. 20. 253-259.Google Scholar
- Furness. D.N., Hackney, C.M. and Steyger, P.S. (1990) The organization of micro tubules in cochlear hair cells. J. Electron Microscop. Tech. 15, 261-279.Google Scholar
- Hackney. C.M. and Furness, D.N. (1987) Hair cell ultrastructure in the normal and kanamycin treated cochlea. Brit. J. Audiol. 21. 319.Google Scholar
- Hackney. C.M. and Furness, D.N. (1989) Observations on the cytoskeleton and related structures of mammalian hair cells. In: Cochlear mechanisms: Structure, function. and models (Eds:Google Scholar
- Wilson, J.P. and Kemp, D.T.) Plenum Press. New York andLondon, pp 11-20.Google Scholar
- Hackney, C.M., Furness, D.N. and Steyger. P.S. (1989) Ultrastructural and immunohistochemical observations on the effects of chronic doses of kanamycin on cochlear hair cells. Abstracts of the 26th Workshop for Inner Ear Biology. p 40.Google Scholar
- Harrison, R V. and Evans, E.F. (1977) The effects of hair cell loss (restricted to outer hair cells) on the threshold and tuning propenies of cochlear fibres in the guinea pig. In: Inner ear biology (Eds: Portmann.l. and Aran. I.M) INSERM, Paris, pp 105 - 124.Google Scholar
- Hawkins, J.E. Jr. (1977) The condition of inner hair cells after aminoglycoside intoxication. In: Inner ear biology (Eds: Portmann, J. and Aran, J.M.) INSERM. Paris, pp 327 - 334.Google Scholar
- Kiang, N.Y.S., Liberman, M. C. Sewell. W. F. and Guinan. J.J. (1986) Single unit clues to cochlear mechanisms. In: Cellular mechanisms in hearing (Eds: Flock, A. and Wersall. 1). Elsevier Science Publishers. Amsterdam, Oxford and New York. pp 171 - 182.Google Scholar
- Patuzzi. R. and Robertson, D. (1988) Tuning in the marnmalian cochlea Physio!. Rev. 68, 1009 - 1082.Google Scholar
- Ryan A.F.. Woolf, N.K. and Bone. R.C. (1980) Ultrastructural correlates of selective outer hair cell destruction following kanamycin intoxication in the chinchilla. Hearing Res. 3, 335 - 351Google Scholar