Abstract
Restriction endonucleases, also called restriction enzymes, are bacterial proteins that cut the long, linear DNA molecule into fragments. Restriction enzymes are a major tool of recombinant DNA technology. A restriction enzyme recognizes a specific nucleotide sequence, such as AGCT, and cuts DNA wherever that combination of “letters” occurs. These enzymes are isolated from bacteria and named with a three- or four-letter sequence followed by a roman numeral. For example, EcoRI is a restriction enzyme isolated from Escherichia coli.
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© 1992 Springer Science+Business Media New York
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Ross, D.W. (1992). Tools of Recombinant DNA Technology. In: Introduction to Molecular Medicine . Springer, New York, NY. https://doi.org/10.1007/978-1-4757-4076-9_3
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DOI: https://doi.org/10.1007/978-1-4757-4076-9_3
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