Abstract
Pneumocystis is a microorganism that causes infection, primarily pneumonia, in immunosuppressed humans and other mammals. Both Chagas in 1909 and Carini in 1910 saw the pneumocystis organism in the lungs of experimental animals infected with trypanosomes. However, both regarded the cysts of Pneumocystis as part of the trypanosome’s life cycle. Shortly afterward the Delanöes reviewed this material and also noted the same cyst-like organism in the lungs of Parisian sewer rats. They recognized the organism as a separate species, and proposed the name Pneumocystis carinii for this organism, describing it in the generic name (Greek: pneumön, lung: kystis, “cyst”) and honoring Carini in the specific designation. At this time Pneumocystis was not recognized as a cause of human disease, although Chagas probably had seen a case of pneumocystis pneumonia in man. Dutz has recounted the discovery of pneumocystis in detail.1
Keywords
Acquire Immune Deficiency Syndrome Acquire Immunodeficiency Syndrome Pneumocystis Pneumonia Arch Pathol Diffuse Alveolar DamagePreview
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References
- 1.Dutz W. Pneumocystis carinii pneumonia. Pathol Annu 1970; 5: 309–341.PubMedGoogle Scholar
- 2.Hughs WT. Pneumocystis carinii: Taxing taxonomy. Eur J Epidemiol 1989; 5: 265–269.CrossRefGoogle Scholar
- 3.Walzer PD. Immunopathogenesis of Pneumocystis carinii infection. J Lab Clin Med 1991;118(3);206–216..PubMedGoogle Scholar
- 4.Edman JC, Kovacs JC, Masur H, Santi DV, Elwood HJ, Sogin ML. Ribosomal RNA sequence shows Pneumocystis carinii to be a member of the fungi. Nature (London) 1988; 334 (6182): 519–522.Google Scholar
- 5.Jackson HC, Colthurst D, Hancock V, Marriott MS, Tuite MF. No detection of characteristic fungal protein elongation factor EF-3 in Pneumocystis carinii. J Infect Dis 1991; 132 (3): 675–677.CrossRefGoogle Scholar
- 6.Gradus MS, Gilmore M, Lerner M. An isolation method of DNA from Pneumocystis carinii: A quantitative comparison to known parasitic protozoan DNA. Comp Biochem Physiol 1988; 89 (1): 75–77.CrossRefGoogle Scholar
- 7.Smith JW, Bartlett MS. In vitro cultivation of pneumocystis. In: Young LS, ed. Pneumocystis carinii pneumonia. New York: Marcel Dekker, 1984: 107–137.Google Scholar
- 8.Sinclair K, Wakefield AE, Banerji S, Hopkin JM. Pneumocystis carinii organisms derived from rat and human hosts are genetically distinct. Mol Biochem Parasitol 1991;45(1)183–184.Google Scholar
- 9.Frenkel JK. Pneumocystis jiroveci n. sp. from man: Morphology, physiology, and immunology in relation to pathology. Natl Cancer Inst Monogr 1976;43:13–27.Google Scholar
- 10.Gajdusek DC. Pneumocystis carinii: Etiologic agent of interstitial plasma cell pneumonia and premature and young infants. Pediatrics 1957;19:543–565.Google Scholar
- 11.Dauzier G, Willis T, Barnett RN. Pneumocystis carinii in an infant. Am J Clin Pathol 1956; 26: 787–793.PubMedGoogle Scholar
- 12.Gajdusek DC. Pneumocystis carinii as the cause of human disease: Historical perspective and magnitude of the problem. Introductory remarks. Natl Cancer Inst Monogr 1976; 43: 1–10.PubMedGoogle Scholar
- 13.Young LS. Clinical aspects of pneumocystosis in man: epidemiology, clinical manifestations, diagnostic approaches, and sequelae. In: Young LS, ed. Pneumocystis carinii pneumonia. New York: Marcel Dekker, 1984: 139–174.Google Scholar
- 14.Hughes WT. Pneumocystis pneumonia: A plague of the immunosuppressed. Johns Hopkins Med J 1978; 184–192.Google Scholar
- 15.Jacobs JL, Libby DM, Winters RA, Gelmont DM, Fried ED, Hartman BJ, Laurence J. A cluster of Pneumocystis carinii pneumonia in adults without predisposing illnesses. N Engl J Med 1991; 324: 246–250.PubMedCrossRefGoogle Scholar
- 16.Burke BA, Good RA. Pneumocystis carinii infection. Medicine (Baltimore) 1973; 52: 23–51.Google Scholar
- 17.Robbins JB. Pneumocystis carinii pneumonitis: A review. Pediatr Res 1967; 1: 131–158.CrossRefGoogle Scholar
- 18.Walzer PD, Schultz MG. Western KA, Robbins JB. Pneumocystis carinii pneumonia and primary immune deficiency diseases. Natl Cancer Inst Monogr 1976; 43: 65–72.Google Scholar
- 19.Richmond DD, Zamvil, Remington JS. Recurrent pneumocystis carinii pneumonia in a child with hypogammaglobulinemia. Am J Dis Child 1973; 125: 102–103.Google Scholar
- 20.Hamlin WB. Pneumocystis carinii. JAMA 1968;204:171172.Google Scholar
- 21.Johnson HD, Johnson WW. Pneumocystis carinii pneumonia with children with cancer: Diagnosis and treatment. JAMA 1970; 214: 1067–1078.PubMedCrossRefGoogle Scholar
- 22.Price RA, Hughes WT. Histopathology of pneumocystis carinii infestation and infection in malignant disease in childhood. Hum Pathol 1974; 5: 737–752.PubMedCrossRefGoogle Scholar
- 23.Rosen P, Armstrong D, Ramos C. Pneumocystis carinii pneumonia. Am J Med 1972; 53: 428–436.PubMedCrossRefGoogle Scholar
- 24.Rifkind D, Faris TD, Hill RB. Pneumocystis carinii pneumonia. Studies on the diagnosis and treatment. Ann Intern Med 1966; 65: 943–956.PubMedCrossRefGoogle Scholar
- 25.Murphy JF, McDonald FD, Dawson M, Reite A, Turcotte J, Fekety R, Jr. Factors affecting the frequency of infection in renal transplant recipients. Arch Intern Med 1976; 136: 670–677.PubMedCrossRefGoogle Scholar
- 26.Washer GF, Schröter GPJ, Starzl TE, Weil R. Causes of death after kidney transplantation. JAMA 1983; 250: 49–54.PubMedCrossRefGoogle Scholar
- 27.Fulginiti VA, Scribner R, Groth CG, et al. Infections in recipients of liver homografts. N Engl J Med 1968; 279: 619–626.PubMedCrossRefGoogle Scholar
- 28.Copeland JG, Mammana RB, Fuller JK, Campbell DW, McAleer MJ, Sailer JA. Heart transplantation: Four year’s experience with conventional immunosuppression. JAMA 1984; 251: 1563–1566.PubMedCrossRefGoogle Scholar
- 29.Pomerance A, Stovin PGI. Heart transplant pathology: The British experience. J Clin Pathol 1985; 38: 146–159.PubMedCrossRefGoogle Scholar
- 30.Winston DJ, Gale RP, Meyer DV, Young LS. Infectious complications of human bone marrow transplantation. Medicine (Baltimore) 1979; 58: 1–31.CrossRefGoogle Scholar
- 31.Masur H, Michelis MA, Greene JB, et al. An outbreak of community-acquired pneumocystis carinii pneumonia: Initial manifestation of cellular immune dysfunction. N Engl J Med 1981; 305: 1431–1438.PubMedCrossRefGoogle Scholar
- 32.Reichert CM, O’Leary TJ, Levens DL, Simrell CR, Macher AM. Autopsy pathology in the acquired immune deficiency syndrome. Am J Pathol 1983; 112: 357–382.PubMedGoogle Scholar
- 33.Guarda LA, Luna MA, Smith JL, Jr., Mansell PW, Gyorkey F, Roca AN. Acquired immune deficiency syndrome: postmortem findings. Am J Clin Pathol 1984; 81: 549–557.PubMedGoogle Scholar
- 34.Hui AN, Koss MM, Meyer PR. Necropsy findings in acquired immunodeficiency syndrome; a comparison of premortem diagnosis with postmortem findings. Hum Pathol 1984; 15: 670–676.PubMedCrossRefGoogle Scholar
- 35.Nash G, Fligiel S. Pathologic features of the lung in acquired immune deficiency syndrome (AIDS): An autopsy study of seventeen homosexual males. Am J Clin Pathol 1984; 81: 6–12.PubMedGoogle Scholar
- 36.Niedt GW, Schinella RA. Acquired immunodeficiency syndrome. Clinicopathologic study of 56 autopsies. Arch Pathol Lab Med 1985;109:727–734.Google Scholar
- 37.Moskowitz L, Hensley GT, Chan JC, Adams K. Immediate causes of death in acquired immunodeficiency syndrome. Arch Pathol Lab Med 1985; 109: 735–738.PubMedGoogle Scholar
- 38.Wallace JM, Hannah JB. Pulmonary disease at autopsy in patients with the acquired immunodeficiency syndrome. West J Med 1988; 149: 167–171.PubMedGoogle Scholar
- 39.McKenzie R, Travis WD, Dolan SA, et al. The causes of death in patients with human immunodeficiency virus infection: A clinical and pathologic study with emphasis on the role of pulmonary diseases. Medicine (Baltimore) 1991; 70: 326–343.CrossRefGoogle Scholar
- 40.Woodward SC, Sheldon WH. Subclinical pneumocystis carinii pneumonitis in adults. Bull Johns Hopkins Hosp 1981; 109: 148–159.Google Scholar
- 41.Shelhamer JH, Ognibene FP, Macher AM, et al. Persistence of pneumocystis carinii in lung tissue of acquired immunodeficiency syndrome patients treated for pneumocystis pneumonia. Am Rev Respir Dis 1984; 130: 1161–1165.PubMedGoogle Scholar
- 42.DeLorenzo LJ, Maguire GP, Wormser GP, Davidian MM, Stone DJ. Persistence of pneumocystis carinii pneumonia in the acquired immune deficiency syndrome. Chest 1985; 88: 79–88.PubMedCrossRefGoogle Scholar
- 43.Pifer LL, Hughes WT, Stagno S, Woods D. Pneumocystis carinii infection: Evidence for high prevalence in normal and immunosuppressed children. Pediatrics 1978; 61: 35–41.PubMedGoogle Scholar
- 44.Jameson B. Serology of pneumocystis carinii. In: Young LS, ed. Pneumocystis carinii pneumonia. New York: Marcel Dekker, 1984; 97–106.Google Scholar
- 45.Meyers JD, Pifer LL, Sale GE, Thomas ED. The value of pneumocystis carinii antibody and antigen detection for diagnosis of pneumocystis carinii pneumonia after marrow transplantation. Ann Rev Respir Dis 1979; 120: 1283–1287.Google Scholar
- 46.Walzer PD, Young LS. Clinical relevance of animal models of pneumocystis carinii pneumonia. Diagn Microbiol Infect Dis 1984; 2: 11–16.CrossRefGoogle Scholar
- 47.Frenkel JK, Good JT, Schultz JA. Latent pneumocystis infection of rats, relapse, and chemotherapy. Lab Invest 1966; 15: 1559–1577.PubMedGoogle Scholar
- 48.Lanken PN, Minda M, Pietra GG, Fishman AP. Alveolar response to experimental pneumocystis carinii pneumonia in the rat. Am J Pathol 1980; 99: 561–588.PubMedGoogle Scholar
- 49.Weber WR, Askin FB, Dehner LP. Lung biopsy in pneumocystis carinii pneumonia: a histopathologic study of typical and atypical features. Am J Clin Pathol 1977; 67: 11–19.PubMedGoogle Scholar
- 50.Hamperl H. Variants of pneumocystis pneumonia. J Pathol Bacteriol 1957; 74: 353–356.CrossRefGoogle Scholar
- 51.Travis WD, Pittaluga S, Lipschik GY. Atypical pathologic manifestations of Pneumocystis carinii pneumonia in the acquired immune deficiency syndrome. Am J Surg Pathol 1990; 14 (7): 615–625.PubMedCrossRefGoogle Scholar
- 52.Askin FB, Katzenstein A-LA. Pneumocystis infection masquerading as diffuse alveolar damage. Chest 1981; 79: 420–422.PubMedCrossRefGoogle Scholar
- 53.LeGolvan DP, Heidelberger KP. Disseminated, granulo-matous pneumocystis carinii pneumonia. Arch Pathol Lab Med 1973; 95: 344–348.Google Scholar
- 54.Crucikshank B. Pulmonary granulomatous pneumocystosis following renal transplantation. Am J Clin Pathol 1975; 63: 384–390.Google Scholar
- 55.Hartz JW, Geisinger KR, Scharyj M, Muss HB. Granulomatous pneumocystosis presenting as a solitary pulmonary nodule. Arch Pathol Lab Med 1985; 102: 466469.Google Scholar
- 56.Bleiweiss IJ, Jagirdar JS, Klein JK, et al. Granulomatous Pneumocystis carinii pneumonia in three patients with the acquired immune deficiency syndrome. Chest 1988; 94: 580–583.PubMedCrossRefGoogle Scholar
- 57.Lee MM, Schinella RA. Pulmonary calcification caused by Pneumoncystis carinii pneumonia. Am J Surg Pathol 1991; 15 (4): 376–380.PubMedCrossRefGoogle Scholar
- 58.Whitcomb ME, Schwarz MI, Charles MA, Larson PH. Interstitial fibrosis after pneumocystis carinii pneumonia. Ann Intern Med 1970; 73: 761–765.PubMedCrossRefGoogle Scholar
- 59.Huang S-N, Marshall KG. Pneumocystis carinii infection: a cytologic, histologic and electron microscopic study of the organism. Am Rev Respir Dis 1970; 102: 623–635.PubMedGoogle Scholar
- 60.Campbell WG Jr. Ultrastructure of pneumocystis in human lung: life cycle in human pneumocystosis. Arch Pathol Lab Med 1972; 93: 312–324.Google Scholar
- 61.Hasleton PS, Curry A, Rankin EM. Pneumocystis carinii pneumonia: a light microscopic and ultrastructural study. J Clin Pathol 1981; 34: 1138–1146.PubMedCrossRefGoogle Scholar
- 62.Hague A, Plattner SB, Cook RT, Hart MN. Pneumocystis carinii. Am J Clin Pathol 1987; 87: 504–510.Google Scholar
- 63.Cushion MT, Ruffolo JJ, Walzer PD. Analysis of the developmental stages of Pneumocystis carinii, in vitro. Lab Invest 1988; 58: 324–331.PubMedGoogle Scholar
- 64.Katzenstein A-LA, Askin FB. Surgical pathology of nonneoplastic lung disease. In: Bennington JL, ed. Major problems in pathology. Vol. 13, 2d Ed. Philadelphia: WB Saunders, 1990: 361.Google Scholar
- 65.Grocott RG. A stain for fungi in tissue sections and smears. Am J Clin Pathol 1955; 25: 975–979.PubMedGoogle Scholar
- 66.Cameron RB, Watts JC, Kasten BL. Pneumocystis carinii pneumonia: an approach to rapid laboratory diagnosis. Am J Clin Pathol 1979; 72: 90–93.PubMedGoogle Scholar
- 67.Watts JC, Chandler FW. Pneumocystis carinii pneumonitis: the nature and diagnostic significance of the methenamine silver-positive “intracystic bodies. ” Am J Surg Pathol 1985; 9: 744–751.PubMedCrossRefGoogle Scholar
- 68.Walker J, Conner G, Ho J, Hunt C, Pickering L. Giemsa staining for cysts and trophozoites of Pneumocystis carinii. J Clin Pathol 1989; 42: 432–434.PubMedCrossRefGoogle Scholar
- 69.Churukian CJ, Schenk EA. Rapid Grocott’s methenamine-silver nitrate method for fungi and pneumocystis carinii. Am J Clin Pathol 1977; 68: 427–428.PubMedGoogle Scholar
- 70.Mahen CT, Sale GE. Rapid methenamine-silver stain for pneumocystis and fungi. Arch Pathol Lab Med 1978; 102: 351–352.Google Scholar
- 71.Churukian CJ, Schenk EA. Dilute ammoniacal silver as a substitute for methenamine silver to demonstrate pneu-Google Scholar
- 72.mocystis carinii and fungi. Lab Med 1986;17:87–90. Blumenfeld W, Griffiss JM. Pneumocystis carinii in sputum. Arch Pathol Lab Med 1988; 112: 814–820.Google Scholar
- 73.Young RC, Bennett JE, Chu EW. Organisms mimicking pneumocystis carinii. Lancet 1976;ii:1082–1083.Google Scholar
- 74.Ma P, Villanueva TG, Kaufman D, Gillooley JF. Respiratory cryptosporidiosis in the acquired immune deficiency syndrome. JAMA 1984; 252: 1298–1301.PubMedCrossRefGoogle Scholar
- 75.Wang N-S, Huang S-N, Thurlbeck WM. Combined pneumocystis carinii and cytomegalovirus infection. Arch Pathol Lab Med 1970; 90: 529–535.Google Scholar
- 76.Graham AR, Sobonya RE, Bronnimann DA, Galgiani JN. Quantiatative pathology of coccidioidomycosis in acquired immunodeficiency syndrome. Hum Pathol 1988; 19: 800–806.PubMedCrossRefGoogle Scholar
- 77.Awen CF, Baltzan MA. Systemic dissemination of pneumocystis carinii pneumonia. Can Med Assoc J 1971; 104: 809–812.PubMedGoogle Scholar
- 78.Cote RJ, Rosenblum M, Telzak EE, May M, Unger PD, Cartun RW. Disseminated Pneumocystis carinii infection causing extrapulmonary organ failure: clinical, pathologic, and immunohistochemical analysis. Mod Pathol 1990; 3: 25–30.PubMedGoogle Scholar
- 79.Raviglione MC. Extrapulmonary pneumocystis: The first 50 cases. Rev Infect Dis 1990; 12 (6): 1127–1138.PubMedCrossRefGoogle Scholar
- 80.Coker RJ, Clark D, Claydon EL, et al. Disseminated Pneumocystis carinii infection in AIDS. J Clin Pathol 1991; 44 (10): 820–823.PubMedCrossRefGoogle Scholar
- 81.DeRoux SJ, Adsay NV, Ioachim HL. Disseminated Pneumocystis without pulmonary involvement during prophylactic aerosolized pentamidine therapy in a patient with the acquired immunodeficiency syndrome. Arch Pathol Lab Med 1991; 115: 1137–1140.PubMedGoogle Scholar
- 82.Beers MF, Sohn M, Swartz M. Recurrent pneumothorax in AIDS patients with Pneumocystis pneumonia. Chest 1990; 98: 266–270.PubMedCrossRefGoogle Scholar
- 83.McClellan MD, Miller SB, Parsons PE, Cohn DL. Pneumothorax with Pneumocystis carinii pneumonia in AIDS. Chest 1991; 100: 1224–1228.PubMedCrossRefGoogle Scholar
- 84.Mariuz P, Raviglione MC, Gould IA, Mullen MP. Pleural Pneumocystis carinii infection. Chest 1991; 99: 774–776.PubMedCrossRefGoogle Scholar
- 85.Limper AH, Offord KP, Smith TF, Martin WJ. Pneumocystis carinii pneumonia: Differences in lung parasite number and inflammation in patients with and without AIDS. Am Rev Respir Dis 1989; 140: 1204–1209.PubMedCrossRefGoogle Scholar
- 86.Pitchenik AE, Ganjei P, Torres A, Evans DA, Rubin E, Baier H. Sputum examination for the diagnosis of pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome. Am Rev Respir Dis 1986; 133: 226229.Google Scholar
- 87.Bigby TD, Margolskee D, Curtis JL, et al. The usefulness of induced sputum in the diagnosis of Pneumocystis carinii pneumonia in patients with the acquired immunodeficiency syndrome. Am Rev Respir Dis 1986; 133: 515–518.PubMedGoogle Scholar
- 88.del Rio C, Guarner J, Honig EG, Slade BA. Sputum examination in the diagnosis of Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome. Arch Pathol Lab Med 1988; 112: 1229–1232.Google Scholar
- 89.Zaman MK, Wooten OJ, Suprahmanya B, Ankobiah W, Finch PJP, Kamholz SL. Rapid noninvasive diagnosis of Pneumocystis carinii from induced liquefied sputum. Ann Intern Med 1988; 109: 7–10.PubMedCrossRefGoogle Scholar
- 90.Leigh TR, Parsons P, Hume C, Husain OAN, Gazzard B, Collins JV. Sputum induction for diagnosis of Pneumocystis carinii pneumonia. Lancet 1989;ii:205–206.Google Scholar
- 91.O’Brien RF, Quinn JL, Miyahara BT, Lepoff RB, Cohn DL. Diagnosis of Pneumocystis carinii pneumonia by induced sputum in a city with moderate incidence of AIDS. Chest 1989; 95: 136–138.PubMedCrossRefGoogle Scholar
- 92.Greaves TS, Strigle SM. The recognition of pneumocystis carinii in routine papanicoalaou-stained smears. Acta Cytol 1985; 29: 714–720.PubMedGoogle Scholar
- 93.Young JA, Stone JW, McGonigle RJS, Adu D, Michael J. Diagnosing Pneumocystis carinii pneumonia by cytological examination of bronchoalveolar lavage fluid: Report of 15 cases. J Clin Pathol 1986; 39: 945–949.PubMedCrossRefGoogle Scholar
- 94.Dugan JM, Avitabile AM, Rossman MD, Ernst CS, Atkinson BF. Diagnosis of Pneumocystis carinii pneumonia by cytologic evaluation of Papanicolaou-stained bronchial specimens. Diagn Cytopathol 1988; 4: 106111.Google Scholar
- 95.Stanley MW, Henry MJ, Iber C. Foamy alveolar casts: Diagnostic specificity for Pneumocystis carinii pneumonia in bronchoalveolar lavage fluid cytology. Diagn Cytopathol 1988; 4: 112–115.Google Scholar
- 96.Naryshkin S, Daniels J, Freno E, Cunningham L. Cytology of treated and minimal Pneumocystis carinii pneumonia and a pitfall of the Grocott methenamine silver stain. Diagn Cytopathol 1991; 7: 41–47.PubMedCrossRefGoogle Scholar
- 97.Sobonya RE, Barbee RA, Wiens J, Trego D. Detection of fungi and other pathogens in immunocompromised patients by bronchoalveolar lavage in an area endemic for coccidioidomycosis. Chest 1990; 97: 1349–1355.PubMedCrossRefGoogle Scholar
- 98.Kovacs JA, Ng VL, Masur H, et al. Diagnosis of pneumocystis carinii pneumonia: Improved detection of sputum with use of monoclonal antibodies. N Engl J Med 1988; 318: 589–593.PubMedCrossRefGoogle Scholar
- 99.Blumenfeld W, Kovacs JA. Use of a monoclonal antibody to detect Pneumocystis carinii in induced sputum and bronchoalveolar lavage fluid by immunoperoxidase staining. Arch Pathol Lab Med 1988; 112: 1233–1236.PubMedGoogle Scholar
- 100.Elvin KM, Björkman, Linder E, Heurlin N, Hjerpe A. Pneumocystis carinii pneumonia: Detection of parasites in sputum and bronchoalveolar lavage fluid by monoclonal antibodies. Br Med J 1988; 297: 381–384.CrossRefGoogle Scholar
- 101.Baughman RP, Strohofer SS, Clinton BA, Nickol AD, Frame PT. The use of an indirect fluorescent antibody test for detecting Pneumocystis carinii. Arch Pathol Lab Med 1989; 113: 1062–1065.PubMedGoogle Scholar
- 102.Midgley J, Parsons PA, Shanson DC, Husain OAN, Francis N. Monoclonal immunofluorescence compared with silver stain for investigating Pneumocystis carinii pneumonia. J Clin Pathol 1991; 44: 75–76.Google Scholar
- 103.Minielly JA, McDuffie, Holley KE. Immunofluorescent identification of pneumocystis carinii. Arch Pathol Lab Med 1970; 90: 561–566.Google Scholar
- 104.Blumefeld W, McCook O, Griffiss JM. Detection of antibodies to Pneumocystis carinii in bronchoalveolar lavage fluid by immunoreactivity to Pneumocystis carinii within alveoli, granulomas, and disseminated sites. Mod Pathol 1992; 5: 107–113.Google Scholar
- 105.Ghali VS, Garcia RL, Skolom J. Fluorescence of pneumocystis carinii in papanicolaou smears. Hum Pathol 1984; 15: 907–909.PubMedCrossRefGoogle Scholar
- 106.Wehle K, Blanke M, Koenig G, Pfitzer P. The cytological diagnosis of Pneumocystis carinii by fluroescence microscopy of Papanicolaou-stained bronchoalveolar lavage specimens. Cytopathology 1991; 2 (3): 113–120.PubMedCrossRefGoogle Scholar
- 107.Stover DE, Zamen MB, Hajdu SI, Lange M, Gold J, Armstrong D. Bronchoalveolar lavage in the diagnosis of diffuse pulmonary infiltrates in the immunosuppressed host. Ann Intern Med 1984; 101: 1–7.PubMedCrossRefGoogle Scholar
- 108.Blumenfeld W, Wagar E, Hadley WK. Use of the trans-bronchial biopsy for the diagnosis of opportunistic pulmonary infections in the acquired immunodeficiency syndrome (AIDS). Am J Clin Pathol 1984; 81: 1–5.PubMedGoogle Scholar
- 109.Stover DE, White DA, Romano PA, Gellene RA. Diagnosis of pulmonary disease in the acquired immune deficiency syndrome (AIDS): role of bronchoscopy and bronchoalveolar lavage. Am Rev Respir Dis 1984; 130: 659–662.PubMedGoogle Scholar
- 110.Swinburn CR, Pozniak AL, Sutherland S, Banks RA, Teall AJ, Johnson NMCI. Early experience and difficulties with bronchoalveolar lavage and transbronchial biopsy in the diagnosis of AIDS-associated pneumonia in Britain. Thorax 1985; 40: 166–170.PubMedCrossRefGoogle Scholar
- 111.Hartman B, Koss M, Hui A, Baumann W, Athos L, Boylen T. Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome (AIDS): diagnosis with bronchial brushings, biopsy, and bronchoalveolar lavage. Chest 1985; 87: 603–607.PubMedCrossRefGoogle Scholar
- 112.Rorat E, Garcia RL, Skolom J. Diagnosis of pneumocystis carinii pneumonia by cytologic examination of bronchial washings. JAMA 1985; 254: 1950–1951.PubMedCrossRefGoogle Scholar
- 113.Pisani RJ, Wright AJ. Clinical utility of bronchoalveolar lavage in immunocompromised hosts. Mayo Clin Proc 1992; 67: 221–227.PubMedCrossRefGoogle Scholar
- 114.Wakefield AE, Pixley FJ, Banerji S, et al. Detection of Pneumocystis carinii with DNA amplification. Lancet 1990; 336: 451–453.PubMedCrossRefGoogle Scholar
- 115.Blumenfeld W, McCook O, Holodniy M, Katzenstein DA. Correlation of morphologic diagnosis of Pneumocystis carinii with the presence of pneumocystis DNA amplified by the polymerase chain reaction. Mod Pathol 1992; 5: 103–106.PubMedGoogle Scholar