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Immunohistochemistry

  • Chapter
Blaustein’s Pathology of the Female Genital Tract

Abstract

In recent years, immunohistochemistry has largely replaced “special stains” and electron microscopy as an adjunctive method to assist in the differential diagnosis of tumors and in the identification of infectious agents. This technique permits visualization of immunologically detectable markers (antigens) in tissue sections; thus, the pathologist can observe both the morphology and immunophenotype of a given tissue or neoplasm. To exploit this technique fully, the pathologist must be acquainted not only with the distribution of various antigens in different tumors and tissue, but also must have sufficient knowledge of the methodology to avoid pitfalls in interpretation. Accordingly, this chapter is divided into a section describing various aspects of the methodology of this technique and a section describing the application of immunohistochemistry in the identification of a variety of antigens, including intermediate filaments, hormone receptors, oncofetal antigens, and gene products, which can be useful in the differential diagnosis and prognosis of tumors of the female genital tract. This latter section is organized by antigens and is intended to complement the individual (organ) chapters. Table 26.1 can assist the reader in selecting which antibodies are useful in the differential diagnosis of various lesions. Appropriate page numbers also are included in the table.

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Authors
  1. Norio Azumi M.D., Ph.D.
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  2. Bernard Czernobilsky M.D.
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Editors and Affiliations

  1. Departments of Gynecology and Obstetrics and Pathology, The Johns Hopkins Hospital and The Johns Hopkins University, School of Medicine, Baltimore, MD, 21287, USA

    Robert J. Kurman M.D.

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Azumi, N., Czernobilsky, B. (1994). Immunohistochemistry. In: Kurman, R.J. (eds) Blaustein’s Pathology of the Female Genital Tract. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-3889-6_26

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