Determination of Subnanomolar Concentrations of Dopamine and Norepinephrine in Nanovolume Samples Using an Automated Capillary Zone Electrophoresis with Laser Induced Fluorescence Detection
Two main types of methods are available for the “in vivo” monitoring of catecholamines (CA) in the brain (review in Kehr, 1999): i) “in vivo” electrochemistry, improperly often referred to as “in vivo” voltammetry, which belongs to the “in situ” methods since they rely on a carbon fiber microlectrode measuring directly the CA within the cerebral tissue and ii) “in vivo” microdialysis technique, which belongs to the “ex situ” methods since it requires the collection of a dialysate which is then analyzed with one or several of the suitable techniques, e.g. HPLC with electrochemical detection for CA.
KeywordsCapillary Electrophoresis Excitatory Amino Acid Circannual Rhythm Brain Microdialysis Concentration Detection Limit
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