Determination of Subnanomolar Concentrations of Dopamine and Norepinephrine in Nanovolume Samples Using an Automated Capillary Zone Electrophoresis with Laser Induced Fluorescence Detection

  • Lionel Bert
  • Valérie Martin
  • Laura Lambas-Señas
  • Marie-Françoise Suaud-Chagny
  • Bernard Renaud
Part of the Advances in Behavioral Biology book series (ABBI, volume 53)

Abstract

Two main types of methods are available for the “in vivo” monitoring of catecholamines (CA) in the brain (review in Kehr, 1999): i) “in vivo” electrochemistry, improperly often referred to as “in vivo” voltammetry, which belongs to the “in situ” methods since they rely on a carbon fiber microlectrode measuring directly the CA within the cerebral tissue and ii) “in vivo” microdialysis technique, which belongs to the “ex situ” methods since it requires the collection of a dialysate which is then analyzed with one or several of the suitable techniques, e.g. HPLC with electrochemical detection for CA.

Keywords

Capillary Electrophoresis Excitatory Amino Acid Circannual Rhythm Brain Microdialysis Concentration Detection Limit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bert, L., Robert, F., Denoroy, L., Stoppini, L., and Renaud, B., 1996, Enhanced temporal resolution for the microdialysis monitoring of catecholamines and excitatory amino acids using capillary electrophoresis with laser-induced fluorescence detection. Analytical developments and in vitro validation, J. Chromatogr. A. 755:99.PubMedCrossRefGoogle Scholar
  2. Bert, L., Parrot, S., Robert, F., Desvignes, C., Suaud-Chagny, M.F., Denoroy, L., and Renaud, B., Submitted, In vivo chronology of rat striatal glutamate, aspartate and dopamine efflux during apomorphine, nomifensine, NMDA and PDC infusion, Neuropharmacology. Google Scholar
  3. Kehr, J., 1999, Monitoring Chemistry of brain microenvironment: Biosensors, microdialysis and related techniques, in: Modern Techniques in Neuroscience Research, U. Windhorst and H. Johansson eds., Springer-Verlag, Berlin, Heidelberg, pp.1149–1149.CrossRefGoogle Scholar
  4. Lada, M.W., and Kennedy, R. T., 1996, Quantitative in vivo monitoring of primary amines in rat caudate nucleus using microdialysis coupled by a flow gated interface to capillary electrophoresis with laserinduced fluorescence detection, Anal. Chem. 68:2790.PubMedCrossRefGoogle Scholar
  5. Morari, M., O’Connor, W. T., Ungerstedt, U., Bianchi, C., and Fuxe, K., 1996, Functional neuroanatomy of the nigrostriatal and striatonigral pathways as studies with dual probe microdialysis in the awake rat-II. Evidence for striatal N-methyl-D-aspartate receptor regulation of striatonigral GABAergic transmission and moto function, Neuroscience 72:89.PubMedCrossRefGoogle Scholar
  6. Newton, A.T., and Justice, J. B., Jr., 1994, Temporal response of microdialysis probes to local perfusion of dopamine and cocaine followed with one-minute sampling, Anal. Chem. 66:1468.PubMedCrossRefGoogle Scholar
  7. Parrot, S., Bert, L., Renaud, B., and Denoroy, L., 2001, Large inter-experiment variations in microdialysate aspartate and glutamate in rat striatum may reflect a circannual rhythm, Synapse, 39:267.PubMedCrossRefGoogle Scholar
  8. Rada, P., Tucci, S., Murzi, E., and Hernandez, L., 1997, Extracellular glutamate increases in the lateral hypothalamus and decreases in the nucleus accumbens during feeding, Brain Res. 768:338.PubMedCrossRefGoogle Scholar
  9. Robert, F., Bert, L., Denoroy, L., and Renaud, B., 1995, Capillary zone electrophoresis with laser-induced fluorescence detection for the determination of nanomolar concentrations of noradrenaline and dopamine: Application to brain microdialysate analysis. Anal. Chem. 67:1838.PubMedCrossRefGoogle Scholar
  10. Robert, F., Bert, L., Lambas-Senas, L., Denoroy, L., and Renaud B., 1996, In vivo monitoring of extracellular noradrenaline and glutamate from rat brain cortex with 2-min microdialysis sampling using capillary electrophoresis with laser-induce fluorescence detection, J. Neurosci. Methods 70:153.PubMedCrossRefGoogle Scholar
  11. Robert, F., Bert, L., Parrot, S., Denoroy, L., Stoppini, L., and Renaud, B., 1998, Coupling on-line brain microdialysis, precolumm derivatization and capillary electrophoresis for routine minute sampling of Ophosphoethanolamine and excitatory amino acids, J. Chromatogr. A. 817:195.PubMedCrossRefGoogle Scholar
  12. Segovia, G., and Mora, F., 1997, Endogenous glutamate increases extracellular concentrations of dopamine, GABA, and taurine through NMDA and AMPA/Kainate receptors in striatum of the freely moving rat: a microdialysis study, J. Neurochem. 69:1476.PubMedCrossRefGoogle Scholar
  13. Westerink, B., 1995, Brain microdialysis and its application for the study of animal behavior, Behav. Brain Res. 70:103.PubMedCrossRefGoogle Scholar
  14. Zilkha, E., Obrenovitch, T. P., Koshy, A., Kusakabe, H., and Bennetto, H. P., 1995, Extracellular glutamate: online monitoring using microdialysis coupled to enzyme-amperometric analysis, J. Neurosci. Methods. 60:1.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Lionel Bert
    • 1
  • Valérie Martin
    • 1
  • Laura Lambas-Señas
    • 1
  • Marie-Françoise Suaud-Chagny
    • 1
  • Bernard Renaud
    • 1
  1. 1.Laboratory of Neuropharmacology and Neurochemistry, School of PharmacyINSERM U512 and Claude Bernard UniversityLyonFrance

Personalised recommendations