Efficient Gene Transfer to Neural Stem Cells by High Titer Retroviral Vectors
Neural stem cells are self-renewing and multipotent cells, and are thought to be ideal sources for neural transplantation.1 However, controlled conversion of these cells into certain types of neurons is difficult. To establish a system for cell fate control or to employ gene manipulation to assign specific functions, the efficiency of retroviral gene transfer was examined. Retroviral transduction offers the advantage of stable gene expression as well as low cytotoxicity. Yet, the vector is limited by the low efficiency of infection. In this study, high titer retrovirus (up to 1012 cfu/ml) was prepared and neural stem cells were transduced efficiently. To test the application of this system in the therapy of Parkinson disease, virus producer cells containing the human tyrosine hydroxylase (HTH) gene were established to prepare high titer TH retrovirus.
KeywordsNeural Stem Cell Stable Gene Expression Packaging Cell Line Adenoviral Transduction Viral Producer Cell
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