Cloning of Novel Genes from the Human Spinal Cord by Molecular Profiling
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease which etiology is unknown. In ALS, large motoneurons in the brain stem and the anterior horn of the spinal cord degenerate selectively and progressively. To uncover the pathogenic mechanism of ALS, it is crucial to identify molecules maintaining motor neuronal integrity or leading to eventual cell death. To this aim, it is necessary to explore the molecules expressed in the spinal cord, and to analyze its function and the interaction they have. Several high throughput methods have been developed to screen many gene expression simultaneously, such as differential displays and cDNA microarrays. Molecular indexing is a method developed by Kato1 to explore the wide range of expressed genes effectively and enables comparison of profiles of gene expression among two or more samples. In the previous study, we have got 70 genes differentially expressed in autopsied Alzheimer’s disease brain compared with normal brain2. In this study, we screened genes expressed in the human spinal cord anterior horn by molecular indexing. Using this technique, we cloned two novel genes, FI58G and FI39A.
KeywordsAmyotrophic Lateral Sclerosis Neurite Outgrowth Neuronal Differentiation Human Spinal Cord Large Motoneuron
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