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Chemical Modification of Wheat Gluten

  • Véronique Tropini
  • Jan-Pleun Lens
  • Wim J. Mulder
  • Françoise Silvestre
Chapter

Abstract

Wheat gluten was cross-linked using water soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HC1 (EDC) together with N-hydroxysuccinimide (NHS) in order to improve its functional properties in non-food applications. By varying reaction parameters, it was demonstrated that cross-linking of the proteins occurred through different mechanisms. The pH-dependent formation of two types of cross-links is suggested. At pH 5–7, intermolecular cross-linking took place, while at pH 11 cross-links were preferentially formed within one protein chain (intramolecular cross-linking). However at pH 3 no reaction was observed.

Keywords

Chemical Modification Protein Chain Wheat Gluten Trinitrobenzenesulfonic Acid Foss Electric 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Lens, J.-P., Mulder, W. J., and Kolster, P., 1999, Modification of wheat gluten for nonfood applications. Cereal Foods World 44: 5–9.Google Scholar
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    Means, G. E., and Feeney, R. E., 1990, Chemical modifications of proteins: history and applications. Bioconjugate Chem. 1: 2–12.CrossRefGoogle Scholar
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    Lundblad, R. L., and Noyes, C. M., 1984, Chemical reagents for protein modification; CRC Press: Boca Raton, FL, Vol. 2.Google Scholar
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    Lens, J.-P., Dietz, C. H. J. T., Verhelst, K.C. S, Vereijken, J. M., and De Graaf, L. A. 1999, Hydrofobization of wheat gluten using acylation and alkylation reactions. Cereal Chemistry submitted.Google Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Véronique Tropini
    • 1
  • Jan-Pleun Lens
    • 2
  • Wim J. Mulder
    • 2
  • Françoise Silvestre
    • 1
  1. 1.Laboratoire de Chimie Agro-industrielleENSCT-INPT, UMR-INRAToulouse Cedex 4France
  2. 2.Subdivision Industrial ProteinsAgrotechnological Research Institute ATOWageningenThe Netherlands

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