The Production of Poly-3-hydroxybutyrate-co-3-hydroxyvalerate with Pseudomonas cepacia ATCC 17759 on Various Carbon Sources
Polyhydroxyalkanoates (PHAs) are biodegradable polyesters which can substitute conventional thermoplastics from non-renewable fossil resources. They are accumulated by a vast number of microorganisms as an intracellular storage product. The material properties of PHB (Polyhydroxybutyrate), the most commonly found PHA, can be improved by adding precursors as for instance 3-Hydroxyvalerate or 4-Hydroxybutyrate during the polymer accumulation phase to the medium generating Poly-3-hydroxybutyrate-co-3-hydroxyvalerate and Poly-3-hydroxybutyrate-co-4-hydroxyvalerate respectively. The main obstacle for a wide use of Polyhydroxyalkanoates as thermoplastics is the high production costs, which can be lowered significantly by using cheap carbon sources, since the cost of the carbon source contributes almost 50 % to the production costs. Cheap carbon sources are waste materials from agricultural and food industry. Whey accumulates in high amounts in cheese-production and therefore causes high waste management costs. Since strain Pseudomonas cepacia ATCC 17759 is capable of both producing PHA and utilizing whey composites lactose and its monomers glucose and galactose as carbon sources the whey could be used for this purpose. Growth kinetics and storage behavior of P. cepacia on various carbon sources and valerate as precursor have been investigated.
KeywordsCarbon Source Specific Growth Rate Ammonium Sulfate Accumulation Phase Residual Biomass
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