Site-Specific Recombination

  • Edward A. Birge


Site-specific recombination occurs in the temperate response of bacteriophages, the integration of plasmids, and the movement of transposons. While there are elements of the basic recombination pathways discussed in Chapter 5, there are unique aspects to each of these processes.


Integration Host Factor Precise Excision Invertible Segment EMBO Journal Inversion System 
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  1. Hallett, B., Sherratt, D.J. (1997). Transposition and site-specific recombination: Adapting DNA cut-and-paste mechanisms to a variety of genetic rearrangements. FEMS Microbiology Reviews 21: 157–178.CrossRefGoogle Scholar
  2. Kim, J., Landy, A. (1992). Lambda Int protein bridges between higher order complexes at two distant chromosomal loci attL and attR. Science 256: 198–203.PubMedCrossRefGoogle Scholar
  3. Kleckner, N. (1990). Regulating Tn 10 and IS 10 transposition. Genetics 124: 449–454.PubMedGoogle Scholar
  4. Lavoie, B.D., Chaconas, G. (1996). Transposition of phage Mu DNA. Current Topics in Microbiology and Immunology 204: 83–102.Google Scholar
  5. Sherratt, D.J., Arciszewska, L.K., Blakely, G., Colloms, S., Grant, K., Leslie, N., McCulloch, R. (1995). Site-specific recombination and circular chromosome segregation. Philosophical Transactions of tbe Royal Society of London, Series B 347: 37–42.CrossRefGoogle Scholar


  1. Benjamin, H.W., Kleckner, N. (1992). Excision of Tn1o from the donor site during transposition occurs by flush double-strand cleavages at the transpo- son termini. Proceedings of the National Academy of Sciences of the USA 89: 4648–4652.PubMedCrossRefGoogle Scholar
  2. Betermier, M., Poquet, I., Alazard, R., Chandler, M. (1993). Involvement of Escherichia coli FIS protein in maintenance of bacteriophage Mu lysogeny by the repressor: Control of early transcription and inhibition of transposition. Journal of Bacteriology 175: 3798–3811.PubMedGoogle Scholar
  3. Chalmers, R., Guhathakurta, A., Benjamin, H., Kleckner, N. (1998). IHF modulation of Tn 10 transposition: Sensory transduction of supercoiling status via a proposed protein/DNA molecular spring. Cell 93: 897–908.PubMedCrossRefGoogle Scholar
  4. Deufel, A., Hermann, T, Kahmann, R., Muskhelishvili, G. (1997). Stimulation of DNA inversion by FIS: Evidence for enhance-independent contacts with the Gin-gix complex. Nucleic Acids Research 25: 3832–3839.PubMedCrossRefGoogle Scholar
  5. Hallet, B., Sherratt, D.J. (1997). Transposition and site-specific recombination: adapting DNA cut-and-paste mechanisms to a variety of genetic rearrangements. FEMS Microbiology Letters 21: 157–178.CrossRefGoogle Scholar
  6. Jain, C. (1997). Models for pairing of IS 10 encoded antisense RNAs in vivo. Journal of Theoretical Biology 186: 431–439.CrossRefGoogle Scholar
  7. Kennedy, A.K., Guhathakurta, A., Kleckner, N., Haniford, D.B. (1998). Tn1o transposition via a DNA hairpin intermediate. Cell 95: 125–134.PubMedCrossRefGoogle Scholar
  8. Klippel, A., Kanaar, R., Kahmann, R., Cozzarelli, N.R. (1993). Analysis of strand exchange and DNA binding of enhancer-independent Gin recombinase mutants. The EMBO Journal 12: 1047–1057.PubMedGoogle Scholar
  9. Kuo, C.-F., Zou, A., Jayaram, M., Getzoff, E., Harshey, R. (1991). DNA—protein complexes during attachment-site synapsis in Mu DNA transposition. The EMBO Journal 10: 1585–1591.PubMedGoogle Scholar
  10. Tirumalai, R.S., Kwon, H.J., Cardente, E.H., Ellenberger, T., Landy, A. (1998). Recognition of core-type DNA sites by lambda integrase. Journal of Molecular Biology 279: 513–527.PubMedCrossRefGoogle Scholar
  11. Yamauchi, M., Baker, T.A. (1998). An ATP-ADP switch in MuB controls progression of the Mu transposition pathway. The EMBO Journal 17: 5509–5518.PubMedCrossRefGoogle Scholar

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© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Edward A. Birge
    • 1
  1. 1.Department of MicrobiologyArizona State UniversityTempeUSA

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