The Analysis of One DNA Sequence

  • Warren J. Ewens
  • Gregory R. Grant
Part of the Statistics for Biology and Health book series (SBH)


Before any analysis of a DNA sequence can take place it is first necessary to determine the actual sequence itself, at least as accurately as is reasonably possible. Unfortunately, technical considerations make it impossible to sequence very long pieces of DNA all at once. Instead, many overlapping small pieces are sequenced, each on the order of 500 bases. After this is done the problem arises of assembling these fragments into one long “contig.” One difficulty is that the locations of the fragments within the genome and with respect to each other are not generally known. However, if enough fragments are sequenced so that there will be many overlaps between them, the fragments can be matched up and assembled. This method is called “shotgun sequencing.”


Variance Formula Geometric Distribution Shotgun Sequencing Homogeneous Poisson Process Joint Density Function 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Warren J. Ewens
    • 1
  • Gregory R. Grant
    • 2
  1. 1.Department of BiologyUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Penn Center for Computational BiologyUniversity of PennsylvaniaPhiladelphiaUSA

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