Abstract
From isomorphous replacement or molecular replacement an approximate model of the protein structure can be obtained in which the broad features of the molecular architecture are apparent. Structure factors calculated on the basis of this model are generally in rather poor agreement with the observed structure factors. The agreement index between calculated and observed structure factors is usually represented by an R-factor as defined in Eq. (13.1). Thus an R-factor of 50% is not uncommon for the starting model, whereas for a random acentric structure it would be 59% (Wilson, 1950).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Drenth, J. (1999). Refinement of the Model Structure. In: Principles of Protein X-ray Crystallography. Springer Advanced Texts in Chemistry. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-3092-0_13
Download citation
DOI: https://doi.org/10.1007/978-1-4757-3092-0_13
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4757-3094-4
Online ISBN: 978-1-4757-3092-0
eBook Packages: Springer Book Archive