Advanced Topics in Fluorescence Quenching

  • Joseph R. Lakowicz


In the previous chapter we described the basic principles of quenching. A wide variety of quenchers are known. Quenching requires molecular contact between the fluorophore and the quencher. This contact can be due to diffusive encounters (dynamic quenching), or to complex formation (static quenching). Because of the close-range interaction required for quenching, the extent of quenching is sensitive to molecular factors that affect the rate and probability of contact, including steric shielding and charge—charge interactions.


Fluorescence Quenching Spin Label Intensity Decay Advanced Topic Excimer Formation 
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© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Joseph R. Lakowicz
    • 1
  1. 1.University of Maryland School of MedicineBaltimoreUSA

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