Fluorescence quenching refers to any process which decreases the fluorescence intensity of a sample. A variety of molecular interactions can result in quenching. These include excited-state reactions, molecular rearrangements, energy transfer, ground-state complex formation, and collisional quenching. In this chapter we will be concerned primarily with quenching resulting from collisional encounters between the fluorophore and quencher, which is called collisional or dynamic quenching. Static quenching is a frequent complicating factor in the analysis of quenching data, but it can also be a valuable source of information about binding between the fluorescent sample and the quencher. In addition to the processes described above, apparent quenching can occur due to the optical properties of the sample. For example, high optical densities or turbidity can result in decreased fluorescence intensities. This is a trivial type of quenching which contains little molecular information. Throughout this chapter, we will assume that such trivial effects are not the cause of the observed decreases in fluorescence intensity.


Fluorescence Quenching Tryptophan Residue Flavin Adenine Dinucleotide Static Quenching Dynamic Quenching 
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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Joseph R. Lakowicz
    • 1
  1. 1.University of Maryland School of MedicineBaltimoreUSA

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