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Advanced Anisotropy Concepts

  • Joseph R. Lakowicz

Abstract

In the preceding two chapters we described steady-state and time-resolved anisotropy measurements and presented a number of biochemical examples which illustrated the types of information available from these measurements. Throughout these chapters, we stated that anisotropy decay depends on the size and shape of the rotating species. However, the theory which relates the form of the anisotropy decay to the shape of the molecule is complex and was not described in detail. In the present chapter we provide an overview of the rotational properties of nonspherical molecules, as well as representative examples.

Keywords

Correlation Time Fluorescence Anisotropy Rotational Diffusion Transition Moment Rotational Correlation Time 
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