Structure and Function of GABA Reuptake Systems
High affinity sodium-coupled transport systems are responsible for the reuptake of neurotransmitters from the synaptic cleft. They appear to play an important role in the overall process of synaptic transmission, namely in its termination (Iversen, 1971, 1973). The process is catalyzed by sodium-coupled neurotransmitter transport systems located in plasma membranes of nerve endings and glial cells (Kanner, 1983, 1989; Kanner and Schuldiner, 1987). These transport systems have been investigated in detail by using plasma membranes obtained upon osmotic shock of synaptosomes. It appears that these transporters are coupled not only to sodium, but also to additional ions like potassium or chloride.
KeywordsDopamine Transporter Plasma Membrane Vesicle Gaba Transporter Neurotransmitter Transporter Glycine Transporter
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- Hoffman, B. J., Mezey, E., and Brownstein, M. J. (1991) Cloning of a serotonin transporter affected by antidepressants. Science 254, 579–580.Google Scholar
- Iversen, L. L. (1971) Role of transmitter uptake mechanism in synaptic neurotransmission. Br. J. Pharmacol. 41, 571–591.Google Scholar
- Kanner, B. I. and Bendahan, A. (1990) Two pharmacologically distinct sodium-and chloride-coupled high-affinity y-aminobutyric acid transporters are present in plasma membrane vesicles and reconstituted preparations from rat brain. Proc. Natl. Acad. Sci. USA 87, 2550–2554.PubMedCrossRefGoogle Scholar
- Keynan, S. and Kanner, B. I. (1988) y-Aminobutyric acid transport in reconstituted preparations from rat brain: coupled sodium and chloride fluxes. Biochemistry 27, 12–17.Google Scholar
- Liu, Q. R., Lopez-Corcuera, B., Nelson, H., Mandiyan, S., and Nelson, N. (1992a) Cloning and expression of a cDNA encoding the transporter of taurine and 0-alanine in mouse brain. Proc. Natl. Acad. Sci. USA 89, 12,145–12, 149.Google Scholar
- Liu, Q. R., Lopez-Corcuera, B., Mandiyan, S., Nelson, H., and Nelson, N. (1993a) Molecular characterization of four pharmacologically distinct y-aminobutyric acid transporters in mouse brain. J. Biol. Chem. 268, 2104–2112.Google Scholar
- Mager, S., Naeve, J., Quick, M., Labarca, C., Davidson, N., and. Lester, H. A. (1993) Steady states, charge movements and rates for a cloned GABA transporter expressed in Xenopus oocytes. Neuron 10, 177–188.Google Scholar