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Nucleic Acid Hybridization and Amplification In Situ

Principles and Applications in Molecular Pathology

  • Chapter
Book cover Molecular Diagnostics

Part of the book series: Pathology and Laboratory Medicine ((PLM))

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Abstract

The ability of pathologists to diagnose disease has been enhanced significantly by the application of nucleic acid technologies, such as Southern blot hybridization and the polymerase chain reaction (PCR). These techniques are used routinely in clinical molecular diagnostic laboratories to detect and analyze genetic alterations associated with human disease. However, the ability to associate the nucleic acid sequence of interest with histopathologic or cytogenetic abnormalities directly is lost when tissue is destroyed during the extraction of nucleic acids. The analysis of nucleic acids in situ overcomes this limitation. ISH* offers the combined advantages of molecular biology, analytical morphology, and cytogenetics by facilitating the analysis of DNA or RNA in tissues and chromosomes. ISA of nucleic acid sequences using PCR is a developing technology that offers increased sensitivity compared to conventional ISH for detecting low-copy sequences. In situ nucleic acid techniques are discussed in detail in this chapter, with emphasis on the principles and clinical relevance of each technique.

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  1. Matthew S. Cowlen
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Editors and Affiliations

  1. Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, NC, USA

    William B. Coleman

  2. Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, CT, USA

    Gregory J. Tsongalis

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Cowlen, M.S. (1997). Nucleic Acid Hybridization and Amplification In Situ . In: Coleman, W.B., Tsongalis, G.J. (eds) Molecular Diagnostics. Pathology and Laboratory Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4757-2588-9_8

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  • DOI: https://doi.org/10.1007/978-1-4757-2588-9_8

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