Abstract
Newborn rats or mice are generally used as the source of tissue for glial cultures. Only about 1% of cells survive the cell disaggregation process and culture environment, and neurons that survive die within the first few days of culturing. Terminally differentiated cells, which can no longer divide, are overgrown by the proliferating, immature cells. As a result, the cells in culture are mainly immature cells and therefore cultures are very plastic. How the cultures will develop and which cell types, i.e., astroglia, Oligodendroglia, ependymal cells, or microglia, will enrich the culture, depends on the culture medium and the physical conditions under which the cells are grown. In addition to varying the components of a chemically defined medium or adding or removing serum from the medium, it is possible to add to the cultures growth factors and/or cytokines in pure recombinant form, or as soluble products in medium conditioned by cells, that produce and secrete the factors. (The latter is considerably cheaper). The addition of such factors to cultures can have dramatic effects. It should be noted that such factors may affect more than one cell type and may initiate different effects in different cells. The factors may interact with other factors synergistically, additively, or in an inhibitory way. Moreover, their half-life is short. This subject is extremely complex and beyond the scope of this book.
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Further Reading
Abd-El-Basset, E. and Fedoroff, S. (1995), Effect of bacterial wall lipopolysaccharide (LPS) on morphology, motility and cytoskeletal organization of microglia in cultures. J. Neurosci. Res. 41, 222–237.
Abd-El-Basset, E. and Fedoroff, S. (1994), Dynamics of actin filaments in microglia during Fc receptor-mediated phagocytosis. Acta Neuropathol. 88, 527–537.
Branch, R. D. and Guilbert, L. J. (1986), Practical in vitro assay systems for the measurement of hematopoietic growth factors. J. Tissue Culture Methods 10, 101–108.
Das, S. K., Stanley, E. R., Guilbert, L. J. and Farman, L. W. (1980), Determination of a colony stimulating factor subclass by a specific receptor on a macrophage cell line. J. Cell Physiol. 104, 359–366.
Frei, K., Siepl, C., Groscurth, P., Bodmer, S., and Fontana, A. (1988), Immunobiology of microglial cells. Adv. Neuroimmunol. Ann. NY Acad. Sci. 540, 218–227.
Fedoroff, S. (1995), Development of microglia, in Neuroglia, Kettenmann, H. and Ransom, B. R., eds., Oxford University Press, pp. 162–181.
Fedoroff, S., Hao, C., Ahmed, J. and Guilbert, L. J. (1993), Paracrine and autocrine signalling in regulation of microglia survival, in Biology and Pathology of Astrocyte-Neuron Interactions, Fedoroff, S., Juurlink, B. H. J., and Doucette, R., eds., Plenum, New York, pp. 247–261.
Gebicke-Haerter, P. J., Baker, J., Schobert, A. and Northoff, H. (1989), Lipopolysaccharide-free conditions in primary astrocyte cultures allow growth and isolation of microglial cells. J. Neurosci. Res. 9, 183–194.
Gehrmann, J., Matsumoto, Y. and Kreutzberg, G. W. (1995), Microglia: intrinsic immune effector cell of the brain. Brain Res. Rev. 20, 269–287.
Giulian, D. and Bauer, T. J. (1986), Characterization of ameboid microglia isolated from developing mammalian brain. J. Neurosci. 6, 2163–2178.
Hao, C., Richardson A., and Fedoroff, S. (1991), Macrophage-like cells originate from neuro-epithelium in culture: characterization and properties of the macrophage-like cells. Int. J. Devi. Neurosci. 9, 1–14.
Hao, C., Guilbert, L. J. and Fedoroff, S. (1990), Production of colony-stimulating factor-1 (CSF-1) by mouse astroglia in vitro. J. Neurosci. Res. 27, 314–323.
Hayes, G. M., Woodroofe, M. N., and Cuzner, M. L. (1988), Characterization of microglia isolated from adult human and rat brain. J. Neuroimmunol. 19, 177–189.
Northoff, H., Gluck, D., Wolpl, A., Kubanek, B., and Galanos, C. (1986a), Lipopolysaccharide induced elaboration of interleukin-1 (IL-1) by human monocytes: Use for the detection of LPS in serum and influence of serum-LPS interactions. Rev. Infect. Dis. 9(Suppl. 5), 599–602.
Northoff, H., Kabelits, D., and Galanos, C. (1986b), Interleukin 1 production for detection of bacterial polysaccharide in fetal calf sera and other solutions. Immunol. Today 7, 126,127.
Perry, V. H. and Gordon, S. (1991), Macrophages and the nervous system. Int. Rev. Cytol. 125, 203–244.
Rieske, E., Graeber, M. B., Tetzlaff, W., Czlonkowska, A., Streit, W. J., and Kreutzberg, G. W. (1989), Microglia and microglia-derived brain macrophages in culture: generation from axotomized rat facial nuclei, identification and characterization in vitro. Brain Res. 429, 1–14.
Streit, W. J. (1995), Microglial cells, in Neuroglia. Kettenmann, H. and Ransom, B. R., eds., Oxford University Press, pp. 85–96.
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Fedoroff, S., Richardson, A. (1997). Generation of Mouse Astroglia and Microglia Cultures from Mouse Neopallium. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4757-2586-5_9
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DOI: https://doi.org/10.1007/978-1-4757-2586-5_9
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