Abstract
The compartmented culture method has numerous features that make it useful for studies of nerve fiber growth, among which are:
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1.
Distal neurites can be exposed to a different fluid and cellular environment than cell bodies and proximal neurites, which is useful for studies of trophic, ionic, and pharmacological regulation of growth;
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2.
Distal neurites can be removed (neuritotomy) and subsequently regenerate, which permits many useful approaches to the study of neurite growth and regeneration; and
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3.
Neurites can be chronically electrically stimulated during growth and regeneration.
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Further Reading
Campenot, R. B. (1979), Independent control of the local environment of somas and neurites, in Methods in Enzymology, vol. 28, Jakoby, W. B. and Pastan, I. H., eds., Academic, New York, pp. 302–307.
Campenot, R. B. (1982), Development of sympathetic neurons in compartmentalized cultures I. Local control of neurite growth by nerve growth factor. Dev. Biol. 93, 1–12.
Campenot, R. B. (1992), Compartmented culture analysis of nerve growth, in Cell-Cell Interactions: A Practical Approach (Stevenson, B., Paul, D., and Gallin, W., eds.), IRL, Oxford, UK, pp. 275–298.
Campenot, R. B. and Draker, D. D. (1989), Growth of sympathetic nerve fibers in culture does not require extracellular calcium. Neuron 3, 733–743.
Hawrot, E., and Patterson, P. H. (1979), Long-term cultures of dissociated sympathetic neurons, in Methods in Enzymology, vol. 28, Jakoby, W. B. and Pastan, I. H., eds., Academic, New York, pp. 574–584.
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Campenot, R.B. (1997). Construction and Use of Compartmented Cultures. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4757-2586-5_7
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DOI: https://doi.org/10.1007/978-1-4757-2586-5_7
Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-4757-2586-5
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