Construction and Use of Compartmented Cultures

  • Robert B. Campenot


The compartmented culture method has numerous features that make it useful for studies of nerve fiber growth, among which are:
  1. 1.

    Distal neurites can be exposed to a different fluid and cellular environment than cell bodies and proximal neurites, which is useful for studies of trophic, ionic, and pharmacological regulation of growth;

  2. 2.

    Distal neurites can be removed (neuritotomy) and subsequently regenerate, which permits many useful approaches to the study of neurite growth and regeneration; and

  3. 3.

    Neurites can be chronically electrically stimulated during growth and regeneration.



Nerve Growth Factor Sympathetic Neuron Stereo Microscope Fine Forceps Silicone Grease 
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Further Reading

  1. Campenot, R. B. (1979), Independent control of the local environment of somas and neurites, in Methods in Enzymology, vol. 28, Jakoby, W. B. and Pastan, I. H., eds., Academic, New York, pp. 302–307.Google Scholar
  2. Campenot, R. B. (1982), Development of sympathetic neurons in compartmentalized cultures I. Local control of neurite growth by nerve growth factor. Dev. Biol. 93, 1–12.PubMedCrossRefGoogle Scholar
  3. Campenot, R. B. (1992), Compartmented culture analysis of nerve growth, in Cell-Cell Interactions: A Practical Approach (Stevenson, B., Paul, D., and Gallin, W., eds.), IRL, Oxford, UK, pp. 275–298.Google Scholar
  4. Campenot, R. B. and Draker, D. D. (1989), Growth of sympathetic nerve fibers in culture does not require extracellular calcium. Neuron 3, 733–743.PubMedCrossRefGoogle Scholar
  5. Hawrot, E., and Patterson, P. H. (1979), Long-term cultures of dissociated sympathetic neurons, in Methods in Enzymology, vol. 28, Jakoby, W. B. and Pastan, I. H., eds., Academic, New York, pp. 574–584.Google Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Robert B. Campenot

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