Aggregating Neural Cell Cultures

  • Paul Honegger
  • Florianne Monnet-Tschudi


Aggregating brain cell cultures are primary, three-dimensional cell cultures consisting of even-sized, spherical structures that are maintained in suspension by constant gyratory agitation. Because the avidity of freshly dissociated fetal cells to attach to their counterparts, cell aggregates form spontaneously and rapidly under appropriate culture conditions. The reaggregated cells are able to migrate within the formed structures, and to interact with each other by direct cell-cell contact, as well as through exchange of nutritional and signaling factors. This tissue-specific environment enables aggregating neural cells to differentiate, and to develop specialized structures (e.g., synapses, myelinated axons) resembling those of brain tissue in situ. Aggregating cell cultures are therefore classified as organotypic cultures (Doyle et al., 1994).


Cholera Toxin Laminar Flow Hood Neuronal Maturation Gyratory Shaker Plastic Pipet 
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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Paul Honegger
  • Florianne Monnet-Tschudi

There are no affiliations available

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