Aggregating Neural Cell Cultures
Aggregating brain cell cultures are primary, three-dimensional cell cultures consisting of even-sized, spherical structures that are maintained in suspension by constant gyratory agitation. Because the avidity of freshly dissociated fetal cells to attach to their counterparts, cell aggregates form spontaneously and rapidly under appropriate culture conditions. The reaggregated cells are able to migrate within the formed structures, and to interact with each other by direct cell-cell contact, as well as through exchange of nutritional and signaling factors. This tissue-specific environment enables aggregating neural cells to differentiate, and to develop specialized structures (e.g., synapses, myelinated axons) resembling those of brain tissue in situ. Aggregating cell cultures are therefore classified as organotypic cultures (Doyle et al., 1994).
KeywordsCholera Toxin Laminar Flow Hood Neuronal Maturation Gyratory Shaker Plastic Pipet
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- Corthésy-Theulaz, L, Mérillat, A.-M., Honegger, P., and Rossier, B.C. (1990), Na+-K+-ATPase gene expression during in vitro development of rat fetal forebrain. Am. J. Physiol. 258 (Cell Physiol. 27), C1062–C1069.Google Scholar
- Doyle, A., Griffiths, J. B., and Newell, D. G. (Principal Editors) (1994), Cell and Tissue Culture: Laboratory Procedures. Wiley, Chichester.Google Scholar
- Honegger, P. and Schilter, B. (1992), Serum-free aggregate cultures of fetal rat brain and liver cells: methodology and some practical applications in neurotoxicology, in The Brain in Bits and Pieces. In vitro Techniques in Neurobiology, Neuropharmacology and Neurotoxicology, Zbinden, G., ed., MTC Verlag, Zollikon, Switzerland, pp. 51–79.Google Scholar