Preparation of Tissue-Cultured Material for Electron Microscopical Observation

  • Richard M. Devon


Electron microscopy is an invaluable tool that has enabled investigators to visualize, at high resolution, the fine structure of cells. Many steps are required in preparing tissue for electron microscopy; these include fixation, dehydration, embedding (infiltration and polymerization), mounting, and sectioning, as well as collection and staining of the sections. At each step, it is possible to introduce artifacts that affect the end results. Therefore, an understanding of the rationale for performing each step (i.e., rates of penetration for various types of fixatives, buffer combinations, infiltration rates, and temperatures used) will assist the investigator in preventing, or at least reducing, the number of possible artifacts that can cause the deterioration of the final image obtained by the electron microscope.


Toluidine Blue Osmium Tetroxide Potassium Ferricyanide Sodium Cacodylate Succinic Anhydride 
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Further Reading

  1. Buckley, I. K. (1971), A simple technique for comparative light and electron microscopy of designated living cultured cells. Lab. Invest. 25, 295–301.PubMedGoogle Scholar
  2. de Bruijn, W. C. and Den Breejen, P. (1975), Glycogen, its chemistry and morphologic appearance in the electron microscope. II. The complex formed in the selective contrast staining of glycogen. Histochem. J. 7, 205.PubMedCrossRefGoogle Scholar
  3. Giammara, B. L. and Hanker, J. S. (1986), Epoxy-slide embedment of cytochemically stained tissues and cultured cells for light and electron microscopy. Stain Technol. 61, 51–58.PubMedGoogle Scholar
  4. Hayat, M. A., ed. (1981), Fixation for Electron Microscopy, Academic, New York.Google Scholar
  5. Humason, G. L., ed. (1979), Animal Tissue Techniques, W. H. Freeman, San Francisco.Google Scholar
  6. Meek, G. A., ed. (1973), Practical Electron Microscopy for Biologists, Wiley-Interscience, London.Google Scholar
  7. Mollenhauer, H. H. (1964), Plastic embedding mixtures for use in electron microscopy. Stain Technol. 39, 111–114.PubMedGoogle Scholar
  8. Reynolds, E. S. (1963), Use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212.PubMedCrossRefGoogle Scholar
  9. Russell, L. and Burguet, S. (1977), Ultrastructure of Leydig cells as revealed by secondary tissue treatment with a ferrocyanide-osmium mixture. Tissue Cell 9, 751.CrossRefGoogle Scholar
  10. Trump, B. F., Smuckler, E. A. and Benditt, E. P. (1961), A method for staining epoxy sections for light microscopy. J. Ultrastruct. Res. 5, 343–348.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Richard M. Devon

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