Procedures for Subculturing Cells
Cells attach to the culture substratum with varying degrees of adherence. Some types of cells do not adhere at all and grow as cell suspensions in the medium. Such cells are referred to as nonadhering cells (nonanchorage-dependent cells). Cells that have to attach to the substratum to proliferate are referred to as adhering cells (anchorage-dependent cells).
KeywordsCulture Vessel Disodium Salt Soybean Trypsin Inhibitor Nonadhering Cell Rubber Policeman
Unable to display preview. Download preview PDF.
- Bottenstein, J. , Hayashi, I., Hutchings, S., Masui, J., Mather, J., McClure, D. B., Ohasa, S., Rizzino, A., Sato, G., Serrero, G., Wolfe, R., and Wu, R. (1979), The growth of cells in serum-free hormone-supplemented media, in Methods in Enzymology Cell Culture, Jakoby, W. B. and Pastan, I. H., eds., Academic, New York, pp. 94–109.Google Scholar
- Melnick, J. L. and Wallis, C. (1977), Problems related to the use of serum and trypsin in the growth of monkey kidney cells, in Developments in Biological Standardization, Perkins, F. T. and Regamey, R. H., eds., S. Karger, Basel, pp. 77–82.Google Scholar
- Waymouth, C. (1982), Methods for obtaining cells in suspension from animal tissues, in Cell Separation Methods and Selected Applications, vol. 1, Pretlow, T. G. and Pretlow, T. P., eds., Academic, New York, 1, 1–29.Google Scholar
- Waymouth, C. (1993), Tissue Dissociation Guide, Worthington Biochemical Corporation, Freehold, NJ, pp. 1–78.Google Scholar