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Procedures for Subculturing Cells

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Abstract

Cells attach to the culture substratum with varying degrees of adherence. Some types of cells do not adhere at all and grow as cell suspensions in the medium. Such cells are referred to as nonadhering cells (nonanchorage-dependent cells). Cells that have to attach to the substratum to proliferate are referred to as adhering cells (anchorage-dependent cells).

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Further Reading

  • Bottenstein, J. , Hayashi, I., Hutchings, S., Masui, J., Mather, J., McClure, D. B., Ohasa, S., Rizzino, A., Sato, G., Serrero, G., Wolfe, R., and Wu, R. (1979), The growth of cells in serum-free hormone-supplemented media, in Methods in Enzymology Cell Culture, Jakoby, W. B. and Pastan, I. H., eds., Academic, New York, pp. 94–109.

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  • Waymouth, C. (1993), Tissue Dissociation Guide, Worthington Biochemical Corporation, Freehold, NJ, pp. 1–78.

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© 1997 Springer Science+Business Media New York

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Richardson, A., Fedoroff, S. (1997). Procedures for Subculturing Cells. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4757-2586-5_17

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  • DOI: https://doi.org/10.1007/978-1-4757-2586-5_17

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-454-9

  • Online ISBN: 978-1-4757-2586-5

  • eBook Packages: Springer Book Archive

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