Skip to main content

Quantification of Cells in Culture

  • Chapter
Book cover Protocols for Neural Cell Culture

Abstract

Cell enumeration using the hemocytometer is applicable when determining the number of cells in a suspension and when the number of samples to be analyzed is relatively small. Hemocytometry is also useful for determining the proportion of singly dispersed cells in a suspension and an estimation of the frequency of viable cells.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Further Reading

  • Bainbridge, D. R. and Macey, M. M. (1983), Hoechst 33258: a fluorescent nuclear counterstain suitable for double-labelling immunofluorescence. J. Immunol. Methods 62, 193–195.

    Article  PubMed  CAS  Google Scholar 

  • Baserga, R. (1989), Measuring parameters of growth, in Cell Growth and Division, Baserga, R., ed., IRL, New York.

    Google Scholar 

  • Borenfreund, E. and Puerner, J. A. (1985a), A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90). J. Tissue Culture Methods 9, 7–9.

    Article  Google Scholar 

  • Borenfreund, E., Puerner, J. A. (1985), Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24, 119–124.

    Article  PubMed  CAS  Google Scholar 

  • Borenfreund, E., Babich, H., Martin-Alguacil, N. (1988), Comparisons of two in vitro cytotoxicity assays—the neutral red (NR) and tetrazolium MTT tests. Toxicol. In Vitro 2, 1–6.

    Article  PubMed  CAS  Google Scholar 

  • Branch, D. R. and Guilbert, L. J. (1986), Practical in vitro assay systems for the measurement of hematopoietic growth factors. J. Tissue Culture Methods 10, 101–108.

    Article  CAS  Google Scholar 

  • Clark, G. (1981), Staining Procedures 4th ed., Williams and Wilkins, Baltimore, MD.

    Google Scholar 

  • Demalsy, P. and Callebaut, M. (1967), Plain water as a rinsing agent preferable to sulfurous acid after the Feulgen nuclear reaction. Stain Technol. 42, 133–136.

    PubMed  CAS  Google Scholar 

  • De Tomasi, J. A. (1936), Improving the technic of the Feulgen stain. Stain Technol. 11, 137–144.

    Google Scholar 

  • Dulbecco, R. and Vogt, M. (1954), Plaque formation and isolation of pure cell lines with poliomyelitis viruses. J. Exp. Med. 99, 167–182.

    Article  PubMed  CAS  Google Scholar 

  • Elias, J. M., Conkling, K., and Makar, M (1972), Cold feulgen hydrolysis: its effect on displacement of tritiated thymidine. Acta Histochem. Cytochem. 5, 125–131.

    Article  Google Scholar 

  • Hamilton, L. H. (1956), Errors in blood cell counting. 1. Technical errors. 2. Statistical errors. Can. J. Med. Tech. 18, 8–14.

    CAS  Google Scholar 

  • Hanks, J. H. and Wallace, R. E. (1958), Determination of cell viability. Proc. Soc. Exper. Biol. Med. 98, 188–192.

    CAS  Google Scholar 

  • Harris, M. (1959), Growth measurements on monolayer cultures with an electronic cell counter. Can. Res. 19, 1020–1024.

    CAS  Google Scholar 

  • Hilwig, I. and Gropp, A. (1972), Staining of constitutive heterochromatin in mammalian chromosomes with a new fluorochrome. Exp. Cell Res. 75, 122–126.

    Article  PubMed  CAS  Google Scholar 

  • Jones, K. H. and Senft, J. A. (1985), An improved method to determine cell viability by simultaneous staining with fluorescein diacetate propidium iodide. J. Hist. Cytol. 33, 77–79.

    Article  CAS  Google Scholar 

  • Kaltenbach, J. P., Kaltenbach, M. H., and Lyons, W. B. (1958), Nigrosin as a dye for differentiating live and dead ascites cells. Exp. Cell Res. 15, 112–117.

    Article  PubMed  CAS  Google Scholar 

  • Kjellstrand, P. T. T. (1977), Temperature and acid concentration in the search for optimum Feulgen hydrolysis conditions. J. Histochem. Cytochem. 25, 129–134.

    Article  PubMed  CAS  Google Scholar 

  • Kolber, M. A., Quinones, R. R., Gress, R. E., and Henkart, P. A. (1988), Measurement of cytotoxicity by target release of the fluorescent dye bis-carboxymethyl-carbosyfluorescein (BCECF). J. Immunol. Meth. 108, 225–264.

    Article  Google Scholar 

  • Latt, S. A. and Stetten, G. (1976), Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J. Histochem. Cytochem. 24, 24–33.

    Article  PubMed  CAS  Google Scholar 

  • McLimans, W. F., Davis, E. V., Glover, F. L., and Rake, G. W. (1957), The submerged culture of mammalian cells: the spinner culture. J. Immunol. 79, 428–433.

    PubMed  CAS  Google Scholar 

  • Moore, P. L., MacCoubrey, I. C., and Haugland, R. P. (1990), A rapid, pH insensitive, two color fluorescence viability (cytotoxicity) assay. J. Cell Biol. 111, 304 (abstract).

    Google Scholar 

  • Mullbacher, A., Parish, C. R., and Mundy, J. P. (1984), An improved colorimetric assay for T cell cytotoxicity in vitro. J. Immun. Methods 68, 205–215.

    Article  CAS  Google Scholar 

  • Parish, C. R., Mullbacher, A. (1983), Automated colorimetric assay for T cell cytotoxicity. J. Immun. Methods 58, 225–237.

    Article  CAS  Google Scholar 

  • Pevzner, L. Z. (1979), Functional Biochemistry of the Neuroglia. Consultants Bureau, New York.

    Book  Google Scholar 

  • Phillips, H. J. and Andrews, R. V. (1959), Some protective solutions for tissue-cultured cells. Exper. Cell Res. 16, 678–682.

    Article  CAS  Google Scholar 

  • Phillips, H. J. and Terryberry, J. E. (1957), Counting actively metabolizing tissue cultured cells. Exper. Cell Res. 13, 341–347.

    Article  CAS  Google Scholar 

  • Roehm, N. W., Rodgers, G. H., Hatfield, S. M., and Glasebrook, A. L. (1991), An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J. Immunol. Methods 142, 257–265.

    Article  PubMed  CAS  Google Scholar 

  • Sanford, K. K., Earle, W. R., Evans, V. J., Walts, H. K., and Shannon, J. E. (1951), The measurement of proliferation in tissue culture by enumeration of cell nuclei. J. Nat. Canc. Inst. 11, 733–795.

    Google Scholar 

  • Schrek, R. (1944), Studies in vitro on the physiology of normal and of cancerous cells. II. The survival and the glycolysis of cells under anaerobic conditions. Arch. Path. 37, 319–327.

    CAS  Google Scholar 

  • Thornthwaite, J. T. and Leif, R. C. (1978), A permanent cell viability assay using Alcian blue. Stain Technology 53, 199–204.

    PubMed  CAS  Google Scholar 

  • Waymouth, C. (1956), A rapid quantitative hematocrit method for measuring increase in cell population of strain L (Earle) cells cultivated in serum-free nutrient solutions. J. Nat. Canc. Inst. 17, 305–313.

    CAS  Google Scholar 

  • Yip, D. K. and Auersperg, N. (1972), The dye exclusion test for cell viability: persistence of differential staining following fixation. In Vitro 7, 323–329.

    PubMed  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Springer Science+Business Media New York

About this chapter

Cite this chapter

Richardson, A., Fedoroff, S. (1997). Quantification of Cells in Culture. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4757-2586-5_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-2586-5_16

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-454-9

  • Online ISBN: 978-1-4757-2586-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics