Culture of Glial Cells from Human Brain Biopsies

  • Voon Wee Yong
  • Jack P. Antel


Surgical resections of selected human brain areas to ameliorate intractable epilepsy provide opportunities to isolate, maintain, and examine nonmalignant human neural cells in vitro. Since these specimens tend to be from patients of early adulthood or older, neurons do not survive the isolation process; the cells extracted are thus of glial origin, and include oligodendrocytes, astrocytes, and microglial cells. In our laboratories, although the biopsy materials (frontal or temporal lobes and corpus callosum) are mostly from subjects undergoing surgery to treat intractable epilepsy, we have not found any differences in properties of cells from such surgery when compared to other types of resections, such as those used to treat vascular anomalies involving the brain. In the frontal and temporal lobe resections, tissue is removed either en bloc or by Cavitron™ ultrasonic aspiration (CUSA), which fragments the tissue into cubes of 2 mm on average. Corpus callosum tissue is always removed by CUSA.


Microglia Cell Adult Human Brain Temporal Lobe Resection Feeding Medium Polycarbonate Tube 
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Further Reading

  1. Armstrong, R., Dorn, H., Kufta, C. V., and Dubois-Dalcq, M. (1990), Do oligodendrocyte precursor cells persist in adult human CNS? 20th Annual Meeting of the Society of Neuroscience, St. Louis, Abstract 402.12.Google Scholar
  2. Barna, B. P., Chou, S. M., Jacobs, B., Yen-Lieberman, B., and Ransohoff, R. M. (1989), Interferon-b impairs induction of HLA-DR antigen expression in cultured adult human astrocytes. J. Neuroimmunol. 23, 45–55.PubMedCrossRefGoogle Scholar
  3. Giulian, D. and Baker, T. J. (1986), Characterization of ameboid microglia isolated from developing mammalian brain. J. Neurosci. 6, 2163–2178.PubMedGoogle Scholar
  4. Kim, S. U. (1985), Antigen expression by glia cells grown in culture. J. Neuroimmunol. 8, 255–282.PubMedCrossRefGoogle Scholar
  5. Kim, S. U., Sato, Y., Silberberg, D. H., Pleasure, D. E., and Rorke, L. (1983), Long-term culture of human oligodendrocytes. Isolation, growth and identification. J. Neurol. Sci. 62, 295–301.PubMedCrossRefGoogle Scholar
  6. McLaurin, J., Antel, J. P., and Yong, V. W. (1995), Immune and non-immune actions of interferon-β-1b on primary human neural cell. Multiple Sclerosis 1, 10–19.PubMedGoogle Scholar
  7. Norton, W. T., Farooq, M., Chiu, F. C., and Bottenstein, J. E. (1988), Pure astrocyte cultures derived from cells isolated from mature brain. Glia 1, 403–414.PubMedCrossRefGoogle Scholar
  8. Prabhakar, S., D’Souza, S., Antel, J. P., McLaurin, J., Schipper, H. M., and Wang, E. (1995), Phenotypic and cell cycle properties of human oligodendrocytes in vitro. Brain Res. 672, 159–169.PubMedCrossRefGoogle Scholar
  9. Raff, M. C. (1989), Glial cell diversification in the rat optic nerve. Science 243, 1450–1455.PubMedCrossRefGoogle Scholar
  10. Raff, M. C., Abney, E. R., Cohen, J., Lindsay, R., and Noble, M. (1983), Two types of astrocytes in cultures of developing rat white matter: differences in morphology, surface gangliosides, and growth characteristics. J. Neurosci. 3, 1289–1300.PubMedGoogle Scholar
  11. Szuchet, S. and Yim, S. H. (1984), Characterization of a subset of oligodendrocytes separated on the basis of selective adherence properties. J. Neurosci. Res. 11, 131–144.PubMedCrossRefGoogle Scholar
  12. Szuchet, S., Stefansson, K., Wollmann, R. L., Dawson, G., and Arnason, B. G. W. (1980), Maintenance of isolated oligodendrocytes in long-term culture. Brain Res. 200, 151–164.PubMedCrossRefGoogle Scholar
  13. Thiele, D. L., Kurosaka, M., and Lipsky, P. E. (1983), Phenotype of the accessory cell necessary for mitogen-stimulated T and B cell responses in human peripheral blood: delineation by its sensitivity to the lysosomotrophic agent, L-leucine methyl ester. J. Immunol. 131, 2282–2290.PubMedGoogle Scholar
  14. Williams, K., Bar-Or, A., Ulvestad, E., Olivier, A., Antel, J. P., and Yong, V. W. (1992), Biology of adult human microglia in culture: comparisons with peripheral blood monocytes and astrocytes. J. Neuropath. Exp. Neurol. 51, 538–549.PubMedCrossRefGoogle Scholar
  15. Yong, V. W., Kim, M. W., and Kim, S. U. (1989), Human glial cells and growth factors, in: Myelination and Demyelination: Implications for Multiple Sclerosis, Kim, S. U., ed., Plenum, New York, pp. 29–48.CrossRefGoogle Scholar
  16. Yong, V. W., Cheung, J. C. B., Uhm, J. H., and Kim, S. U. (1991a), Age-dependent decrease of process formation by cultured oligodendrocytes is augmented by protein kinase C stimulation. J. Neurosci. Res. 29, 87–99.PubMedCrossRefGoogle Scholar
  17. Yong, V. W., Yong, F. P., Ruijs, T. C. G., Antel, J. P., and Kim, S. U. (1991b), Expression and modulation of HLA-DR on cultured human adult astrocytes. J. Neuropath. Exp. Neurol. 50, 16–28.PubMedCrossRefGoogle Scholar
  18. Yong, V. W., Moumdjian, R., Yong, F. P., Ruijs, T. C. G., Freedman, M. S., Cashman, N., and Antel, J. P. (1991c), γ-Interferon promotes proliferation of adult human astrocytes in vitro and reactive gliosis in the adult mouse brain in vivo. Proc. Natl. Acad. Sci. USA 88, 7016–7020.PubMedCrossRefGoogle Scholar
  19. Yong, V. W., Yong, F. P., Olivier, A., Robitaille, Y., and Antel, J. P. (1990), Morphologic heterogeneity of human adult astrocytes in culture: correlation with HLA-DR expression. J. Neurosci. Res. 27, 678–688.PubMedCrossRefGoogle Scholar
  20. Yong, V. W., Dooley, N. P., and Noble, P. G. (1994), Protein kinase C in cultured adult human oligodendrocytes: a potential role for isoform a as a mediator of process outgrowth. J. Neurosci. Res. 39, 83–86.PubMedCrossRefGoogle Scholar

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© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Voon Wee Yong
  • Jack P. Antel

There are no affiliations available

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