Abstract
Several distinct stages of differentiation have been described for the oligodendroglial lineage in vitro (Gard and Pfeiffer, 1990; Gard et al., 1995). These include the actively proliferating bipolar or tripolar oligodendroglial precursor cell, characterized by the presence of GQ1c and GD3 gangliosides in the plasmalemma. The oligodendroglial precursor cell differentiates into the multipolar oligodendroblast, a proliferative cell that has sulfatide but no galactocerebroside in its plasmalemma. The oligodendroblast differentiates into the mitotically quiescent oligodendrocyte, a cell characterized by the presence of galactocerebroside in its plasmalemma. These oligodendrocytes also express other myelin-associated proteins, such as myelin basic protein. When transplanted into the central nervous system (CNS) of hypomyelinating hosts, oligodendrocyte precursors migrate over considerable distances and give rise to large numbers of myelinating oligodendrocytes; oligodendroblasts migrate only short distances and give rise to far fewer myelinating oligodendrocytes (Warrington et al., 1993). When transplanted into the CNS, the mature oligodendrocyte will also myelinate axons (Duncan et al., 1992).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
Further Reading
Behar, T., McMorris, F. A., Novotny, E. A., Barker, J. L., and Dubois-Dalq, M. (1988), Growth and differentiation properties of 0–2A progenitors purified from rat cerebral hemispheres. J. Neurosci. Res. 21, 168–180.
Duncan, I. D., Paino, C., Archer, D. R., and Wood, P. M. (1992), Functional capacities of transplanted cell-sorted adult oligodendrocytes. Dev. Neurosci. 14, 114–122.
Gard, A. L. and Pfeiffer, S. E. (1990), Two proliferative stages of oligodendrocyte lineage (A2B5+O4- and O4+GalC-) under different mitogenic control. Neuron 5, 615–625.
Gard, A. L., Pfeiffer, S. E., and Williams II W. C. (1993), Immunopanning and developmental stage-specific primary culture of oligodendrocyte precursors (O4+ GalC-) directly from postnatal rodent cerebrum. Neuroprotocols 2, 209–218.
Gard, A. L., Williams, W. C., and Burrell, M. R. (1995), Oligodendroblasts distinguished from O-2A glial progenitors by surface phenotype (O4+GalC-) and response to cytokines using signal transducer LIFR beta. Dev. Biol. 167, 596–608.
Hunter, S. F. and Bottenstein, J. E. (1989), Bipotential glial progenitors are targets of neuronal cell-line derived growth factors. Develop. Brain Res. 49, 33–49.
Hunter, S. F. and Bottenstein, J. E. (1990), Growth factor responses of enriched bipotential glial progenitors. Dev. Brain Res. 54, 235–248.
Husain, J. and Juurlink, B. H. J. (1995), Oligodendroglial precursor cell susceptibility to hypoxia is related to poor ability to cope with reactive oxygen species. Brain Res. 698, 86–94.
Louis, J. C., Magal, E., Muir, D., Manthorpe, M., and Varon, S. (1993), CG-4, a new bipotential glial cell line from rat brain, is capable of differentiating in vitro into either mature oligodendrocytes or type-2 astrocytes. J. Neurosci. Res. 31, 193–204.
McCarthy, K. D. and de Vellis, J. (1980), Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J. Cell Biol. 85, 890–902.
Schubert, D., Heinemann, S., Carlisle, W., Tarikas, H., Kines, B., Patrick, J., Steinbach, J. H., Culp, W., and Brandt, B. L. (1974), Clonal cell lines from the rat central nervous system. Nature (Lond.) 249, 224–227.
Szuchet, S., Arnason, B. G. W., and Polak, P. E. (1980), Separation of ovine oligodendrocytes in two distinct bands on a linear sucrose gradient. J. Neurosci. Methods 3, 7–19.
Warrington, A. E., Barbarese, E., and Pfeiffer, S. E. (1993), Differential myelinogenic capacity of specific developmental stages of the oligodendrocyte lineage upon transplantation into hypomyelinating hosts. J. Neurosci. Res. 34, 1–13.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media New York
About this chapter
Cite this chapter
Juurlink, B.H.J., Thorburne, S.K., Devon, R.M. (1997). Generation of Oligodendroblasts from Primary Cultures of Rat Neopallium. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4757-2586-5_10
Download citation
DOI: https://doi.org/10.1007/978-1-4757-2586-5_10
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-454-9
Online ISBN: 978-1-4757-2586-5
eBook Packages: Springer Book Archive