Immobilized Enzyme Reactions on Beads and Membranes
Immobilized biocatalysts have widespread applications in areas like organic synthesis, pollution control and for diagnostic purposes. Enzymes can be immobilized on different supports like polymeric beads, gels, and membranes. Immobilization eliminates the need to separate an enzyme from the product solution and allows these expensive compounds to be reused. In addition, the thermal stability, pH stability, and storage stability of an enzyme may be increased as a result of immobilization.
KeywordsImmobilize Enzyme Spin Label Storage Stability Enzyme Loading Hollow Fiber Membrane
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- 1.R. F. Taylor, “Protein Immobilization: Fundamentals and Applications”, Marcel Dekker, Inc., New York (1991).Google Scholar
- 2.E. Klein, “Affinity Membranes”, John Wiley & Sons, Inc., New York (1990).Google Scholar
- 7.S. Schwimmer, “Source Book of Food Enzymology”, The Avi Publishing Co., Inc. (1981).Google Scholar
- 8.J. R. Whitaker, “Principles of Enzymology for the Food Sciences”, Marcel Dekker, Inc., New York (1972).Google Scholar
- 12.D. I. Metelitsa, E. I. Plyugacheva, V. A. Artomonov and G. M. Baran, Catalytic activity of catalase adsorbed on MIFIL polyamide membranes, Kinetics and Catalysis, 31:1233 (1991).Google Scholar
- 13.S. Vishwanath, W. Huang, L. G. Bachas and D. Bhattacharyya, Site-directed and random enzyme immobilization on functionalized membranes: kinetic studies and models, J. Membrane Sci., In press (1995).Google Scholar
- 14.D. A. Butterfield, J. Lee, S. Ganapathi and D. Bhattacharyya, Biofunctional membranes part IV. Active-site structure and stability of an immobilized enzyme, papain, on modified polysulfone membranes studied by electron paramagnetic resonance and kinetics, J. Membrane Sci. 91: 47 (1994).CrossRefGoogle Scholar
- 15.S. Ganapathi, D. A. Butterfield, D. Bhattacharyya, Flat-sheet and hollow fiber membrane bioreactors: A study of the kinetics and active-site conformational changes of immobilized papain including sorption studies of reaction constituents, J. Chem. Tech. Biotech., In press (1995).Google Scholar