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Affinity Chromatography for Recombinant Proteins

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Book cover Biofunctional Membranes

Abstract

The emergence of biotechnology has provided unique opportunities for the design and high level production of a variety of biomolecules, including recombinant proteins and peptides. Recombinant proteins/peptides with potential therapeutic value are being rapidly developed by the pharmaceutical industry. The purification of these therapeutic proteins must be performed under the most stringent conditions in order to achieve the homogeneity, purity and potency required by Federal regulations. Thus, comes the need for expanding the repertoire of conventional bioseparation techniques with novel methods of purification.

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References

  1. Heng, M.H.; Glatz, C.E. J. Chromatogr. 1995, 689, 227–234.

    Article  CAS  Google Scholar 

  2. Tsuneda, S.; Saito, K.; Furusaki, S.; Sugo, T. J. Chromatogr. 1995, 689, 211–218.

    Article  CAS  Google Scholar 

  3. Ditz, R.; Hauke, G.; Müller, W. Austr. J. Biotechnol. 1991, 5, 101- l02.

    CAS  Google Scholar 

  4. Müller, W. J. Chromatogr. 1990, 510, 133–140.

    Article  Google Scholar 

  5. Kopaciewicz, W.; Rounds, A.; Fausnaugh, J.; Regnier, F.E. J. Chromatogr. 1983, 266, 3–21.

    Article  CAS  Google Scholar 

  6. Regnault, V.; Rivat, C.; Vallar, L.; Geschier, C.; Stoltz, J.F. J. Chromatogr. B 1992, 584, 93–99.

    Article  CAS  Google Scholar 

  7. Dean, P.D.G.; Watson, D.H. J. Chromatogr. 1979, 165, 301–319.

    Article  PubMed  CAS  Google Scholar 

  8. Porath, J.; Carlsson, J.; Olsson, I.; Belfrage, G. Nature 1975, 258, 598–599.

    Article  PubMed  CAS  Google Scholar 

  9. Klein, E. In Afffinity Membranes, Wiley: New York, NY, 1992.

    Google Scholar 

  10. Roper, D.K.; Lightfoot, E.N. J. Chromatogr. 1995, 702, 3–26.

    Article  CAS  Google Scholar 

  11. Chui, W.K.; Wainer, I.W. Anal. Biochem. 1992, 201, 237–245.

    Article  PubMed  CAS  Google Scholar 

  12. Gou, W.; Shang, Z.; Yu, Y.; Guan, Y.; Zhou, L. Biomed. Chromatogr. 1992, 6, 95–98.

    Article  Google Scholar 

  13. Nachman, M. J. Chromatogr. 1992, 597, 167–172.

    Article  PubMed  CAS  Google Scholar 

  14. Nachman, M.; Azad, A.R.; Bailon, P. J. Chromatogr. 1992, 597, 155–166.

    Article  PubMed  CAS  Google Scholar 

  15. Josic, D.; Bal, F.; Schwinn, H. J. Chromatogr. 1993, 632, 1–10.

    Article  PubMed  CAS  Google Scholar 

  16. Wenisch, E.; Vorauer, K.; Jungbauer, A.; Katinger, H.; Righetti, P.G. Electrophoresis 1994, 15, 647–653.

    Article  PubMed  CAS  Google Scholar 

  17. Lütkemeyer, D.; Bretschneider, M.; Büntemeyer, H.; Lehmann, J. J. Chronatogr. 1993, 639, 57–66.

    Article  Google Scholar 

  18. Josic, D.; Reusch, J.; Loster, K.; Baum, O.; Reutter, W. J. Chronatogr. 1992, 590, 59–76.

    Article  CAS  Google Scholar 

  19. Luksa, J.; Menart, V.; Milicic, S.; Kus, B.; Gaberc-Porekar, V.; Josic, D. J. Chronatogr. 1994, 661, 161–168.

    Article  CAS  Google Scholar 

  20. Josic, D.; Lim, Y.P.; Strancar, A.; Reutter, W. J. Chromatogr. B 1994, 662, 217–226.

    Article  CAS  Google Scholar 

  21. van Huynh, N.; Motte, J.C.; Pilette, J.F.; Decleire, M.; Colson, C. Anal. Biochem. 1993, 211, 61–65.

    Article  PubMed  Google Scholar 

  22. Wang, Q.C.; Svec, F.; Fréchet, J.M. Anal. Chem. 1995, 67, 670–674.

    Article  PubMed  CAS  Google Scholar 

  23. Wang, Q.C.; Svec, F.; Fréchet, J.M. J. Chromatogr. 1994, 669, 230–235.

    Article  CAS  Google Scholar 

  24. Wang, Q.C.; Svec, F.; Fréchet, J.M. Anal. Chem. 1993, 65, 2243–2248.

    Article  PubMed  CAS  Google Scholar 

  25. Ford, C.F.; Souminen, I.; Glatz, C.E. Prot. Expres. Purif. 1991, 2, 95–107.

    Article  CAS  Google Scholar 

  26. Smith, J.C.; Derbyshire, R.B.; Cook, F.; Dunthorne, L.; Viney, J.; Brewer, S.J.; Sassenfeld, H.M.; Bell, L.D. Gene 1984, 32, 321–327.

    Article  PubMed  CAS  Google Scholar 

  27. Sassenfeld, H.M.; Brewer, S.J. Bio/Technology 1984, 2, 76–81.

    Article  CAS  Google Scholar 

  28. Brewer, S.J.; Sassenfeld, H.M. Trends Biotechnol. 1985, 3, 119–122.

    Article  CAS  Google Scholar 

  29. Heng, M.H.; Glatz, C.E. J. Chromatogr. 1995, 689, 227–234.

    Article  CAS  Google Scholar 

  30. Hutchens, T.W.; Yip, T.T. Anal. Biochem. 1990, 191, 160–168.

    Article  PubMed  CAS  Google Scholar 

  31. Hochuli, E.; Bannwarth, W.; Döbeli, H.; Gentz, R.; Stüber, D. Bio/Technology 1988, 6, 1321–1325.

    Article  CAS  Google Scholar 

  32. Todd, R.J.; Johnson, R.D.; Arnold, F.H. J. Chronatogr. 1994, 662, 13–26.

    Article  CAS  Google Scholar 

  33. Kasher, M.S.; Wakulchik, M.; Cook, J.A.; Smith, M.C. Biotechniques 1993, 14, 630–641.

    PubMed  CAS  Google Scholar 

  34. Persson, M.; Bergstrand, M.G.; Bülow, L.; Mosbach, K. Anal. Biochem. 1988, 172, 330–337.

    Article  PubMed  CAS  Google Scholar 

  35. Cronan, J.E. J. Biol. Chem. 1990, 265, 10327–10333.

    PubMed  CAS  Google Scholar 

  36. Moks, T.; Abrahmsén, L.; Holmgren, E.; Bilich, M.; Olsson, A.; Uhlén, M.; Pohl, G.; Sterky, C.; Hultberg, H.; Josephson, S.; Holmgren, A.; Jörnvall, H.; Nilsson, B.Biochemistry 1987, 26, 5239–5244.

    Article  PubMed  CAS  Google Scholar 

  37. Schmidt, T.G.M.; Skerra, A. J. Chromatogr. 1994, 676, 337–345.

    Article  CAS  Google Scholar 

  38. Guan, C.; Li, P.; Riggs, P.D. Gene 1988,67, 21–30.

    Article  PubMed  Google Scholar 

  39. Blondel, A.; Bedouelle, H. Eur. J. Biochen. 1990, 193, 325–330.

    Article  CAS  Google Scholar 

  40. Ullmann, A. Gene 1984, 29, 27–31.

    Article  PubMed  CAS  Google Scholar 

  41. Guan, K.; Dixon, J.E.Anal. Biochem. 1991, 192, 262–267.

    Article  PubMed  CAS  Google Scholar 

  42. Field, J.; Nikawa, J.I.; Broek, D.; MacDonald, B.; Rodgers, L.; Wilson, I.A.; Lerner, R.A.; Wigler, M. Mol. Cell. Biol. 1988, 8, 2159–2165.

    PubMed  CAS  Google Scholar 

  43. Hopp, T.P.; Prickett, K.S.; Price, V.L.; Libby, R.T.; March, C.J.; Cerretti, D.P., Urdal, D.L.; Conlon, P.J. Bio/Technologv 1988, 6, 1204–1210.

    Article  CAS  Google Scholar 

  44. Knappik, A.; Pluckthun, A. Biotechniques 1994, 17, 754–761.

    PubMed  CAS  Google Scholar 

  45. Hentz, N.G; Vukasinovic, V.; Daunert, S. in preparation.

    Google Scholar 

  46. Maulet, Y.; Cox, J.A. Biochemistry 1983, 22, 5680–5686.

    Article  PubMed  CAS  Google Scholar 

  47. Tanaka, T.; Ohmura, T.; Hidaka, H. Mol. Pharnacol. 1982, 22, 403–407.

    CAS  Google Scholar 

  48. Levin, R.M.; Weiss, B. Mol. Pharnacol. 1977, 13, 690–697.

    CAS  Google Scholar 

  49. Foster, W.S.; Jarrett, H. W. J. Chromatogr. 1987, 403, 99–107.

    Article  PubMed  CAS  Google Scholar 

  50. Porath, J. Prot. Expr. Purif. 1992, 3, 263–281.

    Article  CAS  Google Scholar 

  51. Seratica, G.C.; Pimbley, J.; Belfort, G. Biotechnol. Bioeng. 1994, 43, 21–36.

    Article  Google Scholar 

  52. Hansen, P.; Lindeberg, G. J. Chronatogr. 1995, 690, 155–159.

    Article  CAS  Google Scholar 

  53. Hansen, P.; Lindeberg, G. J. Chromatogr. 1994, 662. 235–241.

    Article  CAS  Google Scholar 

  54. Wirth, H.J.; Hearn, M.T. J. Chromatogr. 1993, 646, 143–151.

    Article  PubMed  CAS  Google Scholar 

  55. Pearson, R.; Pearson, R.G. (Ed) Hard and Soft Acids and Bases, Dowden, Hutchington & Ross, Stroudsburg, PA 1973.

    Google Scholar 

  56. Díez, S.; Zhou, P.; Daunert, S.; Valiente, M. Anal. Chim. Acta 1995, in press.

    Google Scholar 

  57. Miles, D.; Garcia, A.A. J. Chromatogr. 1995, 702, 173–189.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Chemistry and Center of Membrane Sciences, University of Kentucky, Lexington, KY, 40506-0055, USA

    Nathaniel G. Hentz, Vesna Vukasinovic & Sylvia Daunert

  2. Departament de Química Unitat de Química Analítica, Universitat Autònoma de Barcelona, 08193, Bellaterra (Barcelona), Spain

    Sergi Díez & Manuel Valiente

Authors
  1. Nathaniel G. Hentz
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  2. Vesna Vukasinovic
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  3. Sergi Díez
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  4. Manuel Valiente
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  5. Sylvia Daunert
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Editor information

Editors and Affiliations

  1. University of Kentucky, Lexington, Kentucky, USA

    D. Allan Butterfield

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© 1996 Springer Science+Business Media New York

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Hentz, N.G., Vukasinovic, V., Díez, S., Valiente, M., Daunert, S. (1996). Affinity Chromatography for Recombinant Proteins. In: Butterfield, D.A. (eds) Biofunctional Membranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-2521-6_5

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  • DOI: https://doi.org/10.1007/978-1-4757-2521-6_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4419-3254-9

  • Online ISBN: 978-1-4757-2521-6

  • eBook Packages: Springer Book Archive

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