Cytometry — Cell Analysis

  • Dennis W. Ross

Abstract

Recombinant DNA technology, as reviewed in the last chapter, is designed to detect alterations in the genome down to the level of a single nucleotide alteration. Sometimes, however, we want a broader analysis of the DNA complement of a population of cells. The technology of automated single-cell analysis, flow or image cytometry, fills this need and bridges the gap between molecular analysis and examination of the whole cell. Cytometry is an older technology than recombinant DNA, and has well-established applications in the areas of cancer diagnosis and immunology. Cytometry labs are becoming common in larger medical facilities. This chapter examines the tools of cytometry including the flow and image systems currently available. The biology of the cell division cycle, which is the basis of DNA ploidy and S phase measurements in tumors, is presented first followed by clinical examples in cancer diagnosis. The application of cytometry to immunology is presented briefly and expanded upon in the section on AIDS in Chapter 5.

Keywords

Down Syndrome Cell Analysis Cell Division Cycle Extra Chromosome Image Cytometry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Bibliography

  1. Dressler LG, Bartwo SA (1989) DNA flow cytometry in solid tumors: practical aspects and clinical applications, Semin Diagn Pathol 6: 55 – 82.PubMedGoogle Scholar
  2. Keren DF, Hanson CA, Hurtubise PE (eds) (1994) Flow Cytometry and Clinical Diagnosis. American Society of Clinical Pathologists, Chicago.Google Scholar
  3. Melamed MR, Lindmo T, Mendelsohn ML (eds) (1990) Flow Cytometry and Sorting,2nd ed. Wiley-Liss, NY.Google Scholar
  4. Ross DW (1993) Clinical usefulness of DNA ploidy and cell cycle studies. Arch Pathol Lab Med 117: 1077.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Dennis W. Ross
    • 1
    • 2
  1. 1.The School of Medicine, Department of PathologyUniversity of North Carolina at Chapel HillChapel HillUSA
  2. 2.Department of PathologyForsyth Memorial HospitalWinston-SalemUSA

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