Abstract
This chapter is intended for a later stage in purification efforts, when a method has been established, or a published method has been tried, but there may be some dissatisfaction with the overall procedure. The objectives of a scheme for the purification of an enzyme or other protein may be any or all of the following:
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1.
High overall recovery of activity;
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2.
High degree of purity of the end product;
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3.
Reproducibility
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a.
within the laboratory,
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b.
if scaled up or scaled down,
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c.
in other laboratories;
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a.
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4.
Economical use of reagents and simple apparatus;
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5.
Convenience in terms of working hours.
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© 1994 Springer Science+Business Media New York
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Scopes, R.K. (1994). Optimization of Procedures; Final Steps. In: Protein Purification. Springer Advanced Texts in Chemistry. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-2333-5_12
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DOI: https://doi.org/10.1007/978-1-4757-2333-5_12
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4419-2833-7
Online ISBN: 978-1-4757-2333-5
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