Optimization of Procedures; Final Steps

  • Robert K. Scopes
Part of the Springer Advanced Texts in Chemistry book series (SATC)


This chapter is intended for a later stage in purification efforts, when a method has been established, or a published method has been tried, but there may be some dissatisfaction with the overall procedure. The objectives of a scheme for the purification of an enzyme or other protein may be any or all of the following:
  1. 1.

    High overall recovery of activity;

  2. 2.

    High degree of purity of the end product;

  3. 3.
    1. a.

      within the laboratory,

    2. b.

      if scaled up or scaled down,

    3. c.

      in other laboratories;

  4. 4.

    Economical use of reagents and simple apparatus;

  5. 5.

    Convenience in terms of working hours.



Ionic Strength Ammonium Sulfate Dialysis Tubing Precipitant Concentration Ammonium Sulfate Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Robert K. Scopes
    • 1
  1. 1.Department of Biochemistry and the Centre for Protein and Enzyme TechnologyLa Trobe UniversityBundooraAustralia

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