Two Kinds of Spatially Separated Caffeine-Sensitive Calcium Stores in Smooth Muscle Cells from Guinea-Pig Mesenteric Artery

  • V. A. Buryi
  • D. V. Gordienko
  • M. F. Shuba
Part of the Experimental Biology and Medicine book series (EBAM, volume 26)


Ca2+ stores are known to play an important role in the regulation of cellular Ca2+ and vascular contractility. However, their physiological significance in vascular smooth muscle cells (SMC) is still a subject of much discussion (for references see 17, 18). In contrast to its classical role of releasing Ca2+ to initiate contraction it was proposed that superficially located sarcoplasmic reticulum (SR) can exert a protective Ca2+ buffering action, sequestering Ca2+ ions that enter the cell from extracellular space (7, 19, 20).


Sarcoplasmic Reticulum Outward Current Free Solution Calcium Store Intestinal Smooth Muscle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Ahn, H.Y., H.Karaki and N.Urakawa. Inhibitory effects of caffeine on contraction and calcium movement in vascular and intestinal smooth muscle. Br. J. Pharmacol. 93: 2, 267–274. 1988.PubMedCrossRefGoogle Scholar
  2. 2.
    Benham C.D., Bolton T.B. Spontaneous transient outward currents in single visceral and vascular smooth muscle cells of the rabbit. J. Physiol. Lond. 381: 385–406, 1986.PubMedGoogle Scholar
  3. 3.
    Benham,C.D., T.B.Boiton, R.J.Lany and T.Takewaki. Calcium-activated potassium channels in single smooth muscle cells cf rabbit jejunum and guinea-pig mesenteric artery. J.Physiol.Lond. 371: 45–67, 1986.Google Scholar
  4. 4.
    Buryi,V.A., D.V.Gordienko and M.F.Shuba. Transmembrane ion currents in isolated smooth muscle cells of guinea-pig mesenteric artery. Biol. Membrany 5: 1304–1311, 1988.Google Scholar
  5. 5.
    Buryi,V.A., D.V.Gordienko and M.F.Shuba. Potassium permeability characteristic of single smooth muscle cell membrane from mesenteric artery. Biol. Membrany 9: 595–601, 1992.Google Scholar
  6. 6.
    Cabello,O.A. and W.P.Schilling. Vectorial Ca2+ flux from the extracellular space to the endoplasmic reticulum via a restricted cytoplasmic compartment regulates inositol 1,4,5-trisphosphate-stimulated Ca2+ release from internal stores in vascular endothelial cells. Biochem.J. 295: 357–366, 1993.Google Scholar
  7. 7.
    Casteels, R. and G.Droogmans. Exchange characteristics of the noradrenaline-sensitive calcium store in vascular smooth muscle cells of rabbit ear artery. J. Physiol. Lond. 317: 263–279, 1981.Google Scholar
  8. 8.
    Devine, C.E., A.V.Somlyo and A.P.Somlyo. Sarcoplasmic reticulum and excitation-contraction coupling in mammalian smooth muscle. J. Cell Biol. 52: 690–718, 1972.PubMedCrossRefGoogle Scholar
  9. 9.
    Ganitkevich, V.Ya. and M.F. Shuba. Spontaneous outward currents in the membrane of single isolated smooth muscle cells of coronary artery. Biol. Membrany 5: 1312–1320, 1988.Google Scholar
  10. 10.
    Hamil, O.P., A.Marty, E.Neher, B.Sakmann and F.J.Sigworth. Improved patch-clamp techniques for high resolution current recording from cells and cell free membrane patches. Pflugers Arch. 391: 85–100, 1981.CrossRefGoogle Scholar
  11. 11.
    Hughes, A.D., S.Hering, and T.B.Bolton. The action of caffeine on inward barium current through voltage-dependent calcium channels in single rabbit ear artery cells. Pflugers Arch. 416: 462–466, 1990.PubMedCrossRefGoogle Scholar
  12. 12.
    Hume J.R. and N.Leblanc. Macroscopic K+ currents in single smooth muscle cells of the rabbit portal vein. J.Physiol.Lond. 413: 49–73, 1989.PubMedGoogle Scholar
  13. 13.
    Isenberg,G. and U. Klockner. Calcium tolerant ventricular myocytes prepared by preincubation in a “KB” medium. Pflugers Arch. 395: 6–18, 1982.Google Scholar
  14. 14.
    Nelson M.T., J.B.Patlak, J.F.Worley and N.B.Standen. Calcium channels, potassium channels, and voltage dependence of arterial smooth muscle tone. Am. J. Physiol. 259 (Cell Physiol. 28): C3 - C18, 1990.PubMedGoogle Scholar
  15. 15.
    Ohya Y.,K.Kitamura and H.Kuriyama Cellular calcium regulates outward currents in rabbit intestinal smooth muscle cell. Am. J. Physiol. 252 (Cell Physiol. 21 ): 0401 - C410, 1987.Google Scholar
  16. 16.
    Singer,J.J., and J.V.Walsh Jr. Characterization of calcium-activated potassium channels in single smooth muscle sells using the patch-clamp technique. Pflugers Arch. 408: 2, 98–111, 1987.Google Scholar
  17. 17.
    Sturek, M., K.Kunda and Q.Hu. Sarcoplasmic reticulum buffering of myoplasmic calcium in bovine coronary artery smooth muscle. J. Physiol. Lond. 451: 25–48, 1992.PubMedGoogle Scholar
  18. 18.
    Stehno-Bittel, L., and M.Sturek. Spontaneous sarcoplasmic reticulum calcium release and extrusion from bovine, not porcine, coronary artery smooth muscle. J. Physiol.Lond. 451: 49–78, 1992.PubMedGoogle Scholar
  19. 19.
    van Breemen, C. Calcium requirement for activation of intact aortic smooth muscle. J.Physiol. Lond. 272: 317–329, 1977.PubMedGoogle Scholar
  20. 20.
    van Breemen,C., and K.Saida. Cellular mechanisms regulating [Ca2+]i smooth muscle. Ann. Rev. Physiol. 51: 315–329, 1989.Google Scholar
  21. 21.
    Zholos A.V., L.V.Baidan and M.F.Shuba. The inhibitory action of caffeine on calcium currents in isolated intestinal smooth muscle cells. Pflugers Arch. 419: 267–273, 1991.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • V. A. Buryi
    • 1
  • D. V. Gordienko
    • 1
  • M. F. Shuba
    • 1
  1. 1.A. A. Bogomoletz Institute of PhysiologyUkrainian Academy of Sci.KievUkraine

Personalised recommendations