Conducted Vasomotion in Isolated Arterioles: Evidence for Multiple Cellular Mechanisms

  • Michael P. Doyle
  • Brian R. Duling
Part of the Experimental Biology and Medicine book series (EBAM, volume 26)

Abstract

Many vasoactive agents, when applied to a discrete region of an arteriole, induce responses that spread bidirectionally along the vessel for distances that exceed those that can be accounted for by simple diffusion (6, 8, 17, 18). In the microcirculation, responses to agonists such as acetylcholine and phenylephrine conduct over distances of 2 mm or more (17). Based on the evidence summarized in Table 1, we have initiated a series of experiments designed to explore the hypothesis that conduction is an intrinsic property of the vessel wall and that it is likely an electrotonic spread of membrane potential change through gap junctions. The process of conduction thus represents a form of communication within the arteriolar wall that requires both inter- and intracellular signalling and that is thought to promote homogeneous flow regulation (5, 18). However, neither the intracellular signalling pathways that initiate the conducted signal nor the cell types that propagate the response have been elucidated.

Keywords

Length Constant Vasodilator Response Cheek Pouch Membrane Potential Change Arteriolar Wall 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Chen, G., H. Suzuki, and A. H. Weston. Acetylcholine releases endothelium-derived hyperpolarizing factor and EDRF from rat blood vessels. Br. J. Pharmacol. 95: 1165–1174, 1988.PubMedCrossRefGoogle Scholar
  2. 2.
    Chen, G., Y. Yamamoto, K. Miwa, and H. Suzuki. Hyperpolarization of arterial smooth muscle induced by endothelial humoral substances. Am. J. Physiol. 260 (Heart Circ. Physiol. 29 ): H1888 - H1892, 1991.Google Scholar
  3. 3.
    Delashaw, J. B. and B. R. Duling. Heterogeneity in conducted arteriolar vasomotor response is agonist dependent. Am. J. Physiol. 260 (Heart Circ. Physiol. 29 ): H1276 - H1282, 1991.Google Scholar
  4. 4.
    Doyle, M. P., J. Linden, and B. R. Duling. Nucleoside induced arteriolar constriction: A mast cell dependent response. Am. J Physiol. (Heart Circ. Physiol.) In Press. 1994.Google Scholar
  5. 5.
    Duling, B. R. The role of the resistance arteries in the control of peripheral resistance. In: Resistance arteries, structure and function. Edited by M. J. Mulvany. Elsevier Science Publishers, 1991, p. 3–9.Google Scholar
  6. 6.
    Duling, B. R. and R. M. Berne. Propagated vasodilation in the microcirculation of the hamster cheek pouch. Circ. Res.. 26: 163170, 1970.Google Scholar
  7. 7.
    Duling, B. R., R. W. Gore, R. G. Dacey,Jr., and D. N. Damon. Methods for isolation, cannulation, and in vitro study of single microvessels. Am. J. Physiol. 241 (Heart Circ. Physiol. 10 ): H108 - H116, 1981.Google Scholar
  8. 8.
    Duling, B. R., T. Matsuki, and S. S. Segal. Conduction in the resistance-vessel wall. Contributions to vasomotor tone and vascular communication. In: The Resistance Vasculature, edited by J. A. Bevan, W. Halpern, and M. J. Mulvany. Humana Press, 1991, p. 193–215.Google Scholar
  9. 9.
    Duling, B. R. and R. J. Rivers. Isolation, cannulation, and perfusion of microvessels. In: Microcirculatory Technology,edited by C. H. Baker and W. G. Nastuk. Orlando: Academic Press, 1986, p. 265280.Google Scholar
  10. 10.
    Hirst, G. D. S. and T. O. Neild. Evidence for two populations of excitatory receptors for noradrenaline on arteriolar smooth muscle. Nature 283: 767–768, 1980.PubMedCrossRefGoogle Scholar
  11. 11.
    Hirst, G. D. S. and T. O. Neild. An analysis of excitatory junctional potentials recorded from arterioles. J. Physiol. London. 280: 87104, 1978.Google Scholar
  12. 12.
    Komori, K. and P. M. Vanhoutte. Endothelium-derived hyperpolarizing factor. Blood Vessels 27: 238–245, 1990.PubMedGoogle Scholar
  13. 13.
    Meininger, G. A. and M. J. Davis. Cellular mechanisms involved in the vascular myogenic response. Am. J. Physiol. 263 (Heart Circ. Physiol. 32 ): H747 - H659, 1992.Google Scholar
  14. 14.
    Neild, T. O. and N. Kotecha. Relation beween membrane potential and contractile force in smooth muscle of the rat tail artery during stimulation by norepinephrine, 5-hydroxytryptamine, and potassium. Circ. Res. 60: 791–795, 1987.PubMedCrossRefGoogle Scholar
  15. 15.
    Olesen, S. P., D. E. Clapham, and P. F. Davies. Haemodynamic shear stress activates a K+ current in vascular endothelial cells. Nature 331: 168–170, 1988.PubMedCrossRefGoogle Scholar
  16. 16.
    Robertson, B. E., R. Schubert, J. Hescheler, and M. T. Nelson. cGMP-dependent protein kinase activates Ca-activated K channels in cerebral artery smooth muscle cells. Am. J. Physiol. 265 (Cell Physiol. 34 ): C299 - C303, 1993.Google Scholar
  17. 17.
    Segal, S. S., D. N. Damon, and B. R. Duling. Propagation of vasomotor responses coordinates arteriolar resistances. Am. J Physiol. 25 (Heart Circ. Physiol. 25): H832–H837., 1989.Google Scholar
  18. 18.
    Segal, S. S. and B. R. Duling. Flow control among microvessels coordinated by intercellular conduction. Science 234: 868–870, 1986.PubMedCrossRefGoogle Scholar
  19. 19.
    Segal, S. S. and B. R. Duling. Propagation of vasodilation in resistance vessels of the hamster: development and review of a working hypothesis. Circ. Res. 61: II-20 - II - 25, 1987.Google Scholar
  20. 20.
    Segal, S. S. and B. R. Duling. Conduction of vasomotor responses in arterioles: a role for cell-to-cell coupling?. Am. J Physiol. 256 (Heart Circ. Physiol. 25 ): H838 - H845, 1989.Google Scholar
  21. 21.
    Standen, N. B., J. M. Quayle, N. W. Davies, J. E. Brayden, Y. Huang, and M. T. Nelson. Hyperpolarizing vasodilators activate ATP-sensitive K+ channels in arterial smooth muscle. Science 245: 177–180, 1989.PubMedCrossRefGoogle Scholar
  22. 22.
    Tare, M., H. C. Parkington, H. A. Coleman, T. O. Neild, and G. J. Dusting. Hyperpolarization and relaxation of arterial smooth muscle caused by nitric oxide derived from the endothelium. Nature 346: 69–71, 1990.PubMedCrossRefGoogle Scholar
  23. 23.
    Videbaek, L. M., C. Aalkjaer, and M. J. Mulvany. Pinacidil opens K+-selective channels causing hyperpolarization and relaxation of noradrenaline contractions in rat mesenteric resistance vessels. Br. J Pharmacol. 95: 103–108, 1988.PubMedCrossRefGoogle Scholar
  24. 24.
    Weston, A. H., J. Longmore, D. T. Newgreen, G. Edwards, K. M. Bray, and S. Duty. The potassium channel openers: A new class of vasorelaxants. Blood Vessels 27: 306–313, 1990.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Michael P. Doyle
    • 1
  • Brian R. Duling
    • 1
  1. 1.Department of Molecular Physiology and Biological PhysicsUniversity of Virginia Health Sciences CenterCharlottesvilleUSA

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