Flow-Induced Relaxation of the Rabbit Facial Vein: Role of Endothelium and Subendothelial Tissues

  • X.-H. Xiao
  • John T. Dodge
  • John A. Bevan
Part of the Experimental Biology and Medicine book series (EBAM, volume 26)

Abstract

The aim of this study was to determine if the rabbit facial vein exhibits flow-induced relaxation when the vascular wall tone is raised, whether this relaxation is exclusively dependent on the endothelium, and whether the endothelial component of relaxation is due to the release of EDRF. When the vascular wall tone was raised by varying concentrations of histamine, the flow-induced relaxation that occurred was a constant proportion of the level of active tone. This relaxation was attenuated but not eliminated by L-NNA and methylene blue in concentrations which specifically abolished the endothelium-dependent relaxation of acetylcholine but had no effect on that due to papaverine. Coadministration of L-arginine prevented the effect of LNNA. However, after physical removal of the endothelium, established by loss of relaxation to acetylcholine and comprehensive scanning electron microscopy, flow-induced relaxation was diminished but not abolished. At 2Oµ1/min it was 68 ± 15% of that in endothelium-intact vessels. It is concluded that flow-induced relaxation of the rabbit facial vein, occurs when wall tone is raised by histamine and that it originates from both the endothelial and subendothelial sites. The endothelial component appears to depend upon the release of EDRF.

Keywords

Methylene Blue Physiological Salt Solution Intrapulmonary Artery Papaverine Hydrochloride Endothelium Removal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • X.-H. Xiao
    • 1
  • John T. Dodge
    • 1
  • John A. Bevan
    • 1
  1. 1.Department of PharmacologyUniversity of VermontBurlingtonUSA

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