Production of Monoclonal Antibodies against Low-Abundance Ion-Channel-Associated Proteins in Neuronal Tissue
The voltage-sensitive calcium channel is indispensable to the regulation of intracellular calcium concentration. Several types of calcium channels can be distinguished by electrophysiological and pharmacological properties (Bean, 1989; Hess, 1990). A dihydropyridine (DHP)-sensitive L-type calcium channel has been purified from transverse tubules of rabbit skeletal muscle and found to be made up of α 1, α 2,β, γ, and δ subunits (Catterall et al.,1988). The cDNAs of all of these subunits have been cloned and their amino acid sequences determined (Tanabe et al., 1987; Ellis et al., 1988; Ruth et al., 1989; Jay et al., 1990). The cDNAs of the skeletal α 1, subunit have been used as molecular probes, and four different α 1, subunit genes have been found to be expressed in the brain (Snuch et al., 1990). Further multiple subtypes are generated by alternative splicing, thus indicating various kinds of calcium channels to be present in the organ. The subunit structures and properties of these multiple calcium channels should be studied to provide some clarification of their roles in brain functions. It is quite difficult, however, to purify neuronal calcium channels, since they are present in very small quantities. The existence of many homologous structures among calcium channel subtypes makes purification even more difficult.
KeywordsCalcium Channel Synaptic Membrane Chick Brain Brain Synaptic Membrane Calcium Channel Subtype
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