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Reconstitution of Muscle Calcium Channel Function in Bilayer Membranes

From the First Steps to Results

  • Chapter
Book cover Methods in Pharmacology

Abstract

Ion channels are membrane proteins that are used by the cell as signal transducers and as a pathway for the rapid entry of some regulatory compounds such as calcium ions. The behavior of a single channel can be monitored because, when open, the channel passes ~106 ions/sec. This is enough current to measure electrically using patch-clamp techniques (Hamill et al., 1981; Sakmann and Neher, 1984). With standard voltage-clamp techniques (Hille, 1984), it was essentially impossible to measure the small currents generated by the opening of single channels because the signal is too small in comparison to the noise generated by the rest of the system. Patch-clamp recordings are made from very small membrane areas; this improves the signal-to-noise ratio and therefore makes it possible to measure the small single-channel currents.

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  1. Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, Canada

    Dieter J. Pelzer, Terence F. McDonald & Siegried Pelzer

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  1. Universität Innsbruck, Innsbruck, Austria

    Hartmut Glossmann  & Jörg Striessnig  & 

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Pelzer, D.J., McDonald, T.F., Pelzer, S. (1993). Reconstitution of Muscle Calcium Channel Function in Bilayer Membranes. In: Glossmann, H., Striessnig, J. (eds) Methods in Pharmacology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-2239-0_5

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