A Highly Porous Sodium Dodecyl Sulfate-Polyacrylamide Gel System

A Versatile Technique for Protein Structure and Function Study
  • Jean-Pierre Doucet
  • Brian J. Murphy
  • Balwant S. Tuana
Chapter

Abstract

The major contributions to the development of protein separation by gel electrophoresis were the introduction of polyacrylamide gels (Raymond and Weintraub, 1959) and of discontinuous buffer systems (Davis, 1964; Ornstein, 1964), the use of the powerful detergent sodium dodecyl sulfate (SDS) to solubilize protein complexes prior to electrophoresis (Summers et al., 1965), and the addition of SDS to discontinuous buffer systems (Laemmli, 1970; Neville, 1971). In 1977, Porzio and Pearson (1977) described a highly porous SDS-polyacrylamide gel which gave an increased resolution over a wide range of polypeptide molecular weights (MWs). This latter contribution was originally introduced as a continuous disk electrophoresis method.

Keywords

Chromaffin Cell Ammonium Persulfate Rabbit Skeletal Muscle Tank Buffer Discontinuous Buffer System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Jean-Pierre Doucet
    • 1
  • Brian J. Murphy
    • 2
  • Balwant S. Tuana
    • 1
  1. 1.Department of PharmacologyUniversity of OttawaOttawaCanada
  2. 2.Department of Pharmacology, SJ-30University of WashingtonSeattleUSA

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