The Prokaryotes pp 3137-3143 | Cite as

The Genus Acinetobacter

  • Kevin J. Towner


Members of the genus Acinetobacter have previously been classified by various authors under a variety of different names (reviewed by Henriksen, 1973), and, consequently, much of the early literature concerning this group of organisms is difficult to interpret owing to confusion over nomenclature and the lack of a widely accepted classification scheme. The genus Acinetobacter originally proposed by Brisou and Prévot (1954) included a heterogenous collection of nonmotile Gram-negative saprophytes that could be distinguished from other similar bacteria by their lack of pigmentation (Ingram and Shewan, 1960). The Subcommittee on the Taxonomy of Moraxella and Allied Bacteria subsequently proposed (Lessel, 1971) that the genus Acinetobacter should include only the oxidasenegative strains. This division has been supported by the use of transformation tests (Juni, 1972), which now forms the basis for inclusion in the genus. Acinetobacter is classified in the family Neisseriaceae, and the current generic description (Juni, 1984) allows unambiguous identification of strains to the genus level.


Semisolid Medium Acinetobacter Calcoaceticus Mixed Bacterial Culture Acinetobacter Strain Chicken Carcass 
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Literature Cited

  1. Ahlquist, E. E, C. A. Fewson, D. A. Ritchie, J. Podmore, and V. Rowell. 1980. Competence for genetic transformation in Acinetobacter calcoaceticus NCIB 8250. FEMS Microbiol. Lett. 7: 107–109.CrossRefGoogle Scholar
  2. Al-Khoja, M. S. and J. H. Darrell. 1979. The skin as the source of Acinetobacter and Moraxella species occurring in blood cultures. J. Clin. Pathol. 32: 497–499.PubMedCrossRefPubMedCentralGoogle Scholar
  3. Barker, J. and H. Maxsted. 1975. Observations on the growth and movement of Acinetobacter on semi-solid media. J. Med. Microbiol. 8: 443–446.PubMedCrossRefGoogle Scholar
  4. Barnes, E. M. and M. J. Thornley. 1966. The spoilage of eviscerated chickens stored at different temperatures. J. Food Technol. 1: 113–119.CrossRefGoogle Scholar
  5. Baumann, R. 1968. Isolation of Acinetobacter from soil and water. J. Bacteriol. 96: 39–42.PubMedPubMedCentralGoogle Scholar
  6. Baumann, R, M. Doudoroff, and R. Y. Stanier. 1968. A study of the Moraxella group. II. Oxidase-negative species (genus Acinetobacter). J. Bacteriol. 95: 1520–1541.PubMedPubMedCentralGoogle Scholar
  7. Bergogne-Bérézin, E., M. L. Joly-Guillou, and J. F. Vieu. 1987. Epidemiology of nosocomial infections due to Acinetobacter calcoaceticus. J. Hosp. Infect. 10: 105–113.PubMedCrossRefGoogle Scholar
  8. Blaise, C. R. and J. B. Armstrong. 1973. Lipolytic bacteria in the Ottawa river. Appl. Microbiol. 26: 733–740.PubMedPubMedCentralGoogle Scholar
  9. Blouse, L. and R. Twarog. 1966. Properties of four Herellea phages. Can. J. Microbiol. 12: 1023–1030.PubMedCrossRefGoogle Scholar
  10. Bouvet, R. J. M. and P. A. D. Grimont. 1986. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp.nov., Acinetobacter haemolyticus sp.nov., Acinetobacter johnsonii sp.nov., and Acinetobacter junii sp.nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter 1 wofii. Int. J. Sys. Bacteriol. 36: 228–240.CrossRefGoogle Scholar
  11. Bouvet, R. J. M. and S. Jeanjean. 1989. Delineation of new proteolytic genomic species in the genus Acinetobacter. Res. Microbiol. 140: 291–299.PubMedCrossRefGoogle Scholar
  12. Breuil, C. and D. J. Kushner. 1975. Lipase and esterase formation by psychrophilic and mesophilic Acinetobacter spp. Can. J. Microbiol. 21: 423–433.PubMedCrossRefGoogle Scholar
  13. Brisou, J. and A. R. Prévot. 1954. Etudes de systematique bacterienne. X. Revision des espéces réunies dans le genre Achromobacter. Ann. Inst. Pasteur 86: 722–728.Google Scholar
  14. Brooks, K. and T. Sodeman. 1974. Clinical studies on a transformation test for identification of Acinetobacter (Mima and Herellea). Appl. Microbiol. 27: 1023–1026.PubMedPubMedCentralGoogle Scholar
  15. Buxton, A. E., R. L. Anderson, D. Werdegar, and E. Atlas. 1978. Nosocomial respiratory tract infection and colonization with Acinetobacter calcoaceticus. Amer. J. Med. 65: 507–513.PubMedCrossRefGoogle Scholar
  16. Chopade, B. A., R J. Wise, and K. J. Towner. 1985. Plasmid transfer and behaviour in Acinetobacter calcoaceticus EBF65/65. J. Gen. Microbiol. 131: 2805–2811.PubMedGoogle Scholar
  17. Cohen-Bazire, G., W. R. Sistrom, and R. Y. Stanier. 1957. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J. Cellular Comp. Physiol. 49: 25–68.CrossRefGoogle Scholar
  18. Cruze, J. A., J. T. Singer, and W. R. Finnerty. 1979. Conditions for quantitative transformation in Acinetobacter calcoaceticus. Curr. Microbiol. 3: 129–132.CrossRefGoogle Scholar
  19. Cunha, B. A., J. J. Klimek, J. Gracewski, J. C. McLaughlin, and R. Quintiliani. 1980. A common source outbreak of Acinetobacter pulmonary infections traced to Wright respirometers. Postgrad. Med. J. 56: 169–172.PubMedCrossRefPubMedCentralGoogle Scholar
  20. Ditta, G., T. Schmidhauser, E. Yakobsen, R Lu, X. Wuliang, D. R. Findlay, D. Guiney, and D. Helinski. 1985. Plasmids related to the broad host range vector pRK290 useful for gene cloning and for monitoring gene expression. Plasmid 13: 149–153.PubMedCrossRefGoogle Scholar
  21. Dowda, H. 1977. Evaluation of two rapid methods for identification of commonly encountered nonfermenting or oxidase-positive Gram-negative rods. J. Clin. Microbiol. 6: 605–609.Google Scholar
  22. Droop, M. R. and H. W. Jannasch. 1977. Bacterial indication of water pollution. Adv. Aquatic Microbiol. 1: 346–352.Google Scholar
  23. Eribo, B. E. and J. M. Jay. 1985. Incidence of Acinetobacter species and other Gram-negative, oxidase-negative bacteria in fresh and spoiled ground beef. Appl. Environ. Microbiol. 49: 256–257.PubMedPubMedCentralGoogle Scholar
  24. Fewson, C. A. 1981. Biodegradation of aromatics with industrial relevance, p. 141–179. In: T. Leisinger, R. Hilt-ter, A. M. Cook, and J. Nüesch (ed.), Microbial degradation of xenobiotics and recalcitrant compounds. Academic Press, London.Google Scholar
  25. Foght, J. M., D. L. Gutnick, and D. W. S. Westlake. 1989. Effect of emulsan on biodegradation of crude oil by pure and mixed bacterial cultures. Appl. Environ. Microbiol. 55: 36–42.PubMedPubMedCentralGoogle Scholar
  26. French, G. L., M. W. Casewell, A. J. Roncoroni, S. Knight, and I. Phillips. 1980. A hospital outbreak of antibiotic-resistant Acinetobacter anitratus: epidemiology and control. J. Hosp. Infect. 1: 125–131.Google Scholar
  27. Gardner, G. A. 1971. Microbiological and chemical changes in lean Wiltshire bacon during aerobic storage. J. Appl. Bacteriol. 34: 645–654.PubMedCrossRefGoogle Scholar
  28. Gilardi, G. L. 1973. Nonfermentative gram-negative bacteria encountered in clinical specimens. Ant. van Leuwenhoek J. Microbiol. Serol. 39: 229–242.CrossRefGoogle Scholar
  29. Ginther, C. L. 1977. Genetic analysis of Acinetobacter calcoaceticus proline auxotrophs. J. Bacteriol. 133: 439–441.Google Scholar
  30. Glew, R. H., R. C. Moellering, and L. J. Kunz. 1977. Infections with Acinetobacter calcoaceticus (Herellea vaginicola): clinical and laboratory studies. Medicine 56: 79–97.PubMedCrossRefGoogle Scholar
  31. Gutnick, D. L. and W. Minas. 1987. Perspectives on microbial surfactants. Biochem. Soc. Trans. 15: 22S - 35S.PubMedGoogle Scholar
  32. Hauge, J. G. 1960. Purification and properties of glucose dehydrogenase and cytochrome b from Bacterium anitratum. Biochim. Biophys. Acta. 45: 250–262.PubMedCrossRefGoogle Scholar
  33. Henrichsen, J. and J. Blom. 1975. Correlation between twitching motility and possession of polar fimbriae in Acinetobacter calcoaceticus. Acta. Path. Microbiol. Scand. 83B: 103–115.Google Scholar
  34. Henriksen, S. D. 1973. Moraxella, Acinetobacter, and the Mimiae. Bacteriol. Rev. 37: 522–561.PubMedPubMedCentralGoogle Scholar
  35. Herman, N. J. and E. Juni. 1974. Isolation and characterization of a generalized transducing bacteriophage for Acinetobacter. J. Virol. 13: 46–52.PubMedPubMedCentralGoogle Scholar
  36. Hinchliffe, E. and A. Vivian. 1980. Naturally occurring plasmids in Acinetobacter calcoaceticus: a P class R factor of restricted host range. J. Gen. Microbiol. 116: 75–80.Google Scholar
  37. Holton, J. 1983. A note on the preparation and use of a selective differential medium for the isolation of Acinetobacter spp. from clinical specimens. J. Appl. Bacteriol. 54: 141–142.CrossRefGoogle Scholar
  38. Ingram, M. and J. W. Shewan. 1960. Introductory reflections on the Pseudomonas-Achromobacter group. J. Appl. Bacteriol. 23: 373–378.CrossRefGoogle Scholar
  39. Jay, J. M. 1982. Modern food microbiology, 3rd ed. Van Nostrand, New York.Google Scholar
  40. Johnson, J. L., R. S. Anderson and E. J. Ordal. 1970. Nucleic acid homologies among oxidase-negative Moraxella species. J. Bacteriol. 101: 568–573.PubMedPubMedCentralGoogle Scholar
  41. Juni, E. 1972. Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. J. Bacteriol. 112: 917–931.PubMedPubMedCentralGoogle Scholar
  42. Juni, E. 1978. Genetics and physiology of Acinetobacter. Ann. Rev. Microbiol. 32: 349–371.Google Scholar
  43. Juni, E. 1984. Genus III. Acinetobacter Brisou et Prévot 1954, p. 303–307. In: N. R. Krieg and J. G. Holt (ed.), Bergey’s manual of systematic bacteriology, vol. 1. Williams and Wilkins, Baltimore.Google Scholar
  44. Juni, E. and A. Janik. 1969. Transformation of Acinetobacter calcoaceticus (Bacterium anitratum). J. Bacteriol. 98: 281–288.PubMedPubMedCentralGoogle Scholar
  45. Jyssum, K. and R. E. Joner. 1965. Regulation of the nitrogen assimilation from nitrate and nitrite in Bacterium anitratum (B5W). Acta. Pathol. Microbiol. Scand. 64: 387–397.Google Scholar
  46. Koburger, S. A. 1964. Isolation of Mima polymorpha from dairy products. J. Dairy Sci. 47: 646.CrossRefGoogle Scholar
  47. LaCroix, S. J. and V. J. Cabelli. 1982. Membrane filter method for enumeration of Acinetobacter calcoaceticus from environmental waters. Appl. Environ. Microbiol. 43: 90–96.PubMedPubMedCentralGoogle Scholar
  48. Lahellec, C., C. Meurier, G. Bennejean, and M. Castarat. 1975. A study of 5920 strains of psychrophilic bacteria isolated from chickens. J. Appl. Bacteriol. 38: 89–97.PubMedCrossRefGoogle Scholar
  49. Lehmann, V. 1971. Phospholipase activity of Acinetobacter calcoaceticus. Acta. Pathol. Microbiol. Scand. 79B: 372–376.Google Scholar
  50. Lessel, E. F. 1971. Minutes of the Subcommittee on the Taxonomy of Moraxella and Allied Bacteria. Int. J. Syst. Bacteriol. 21: 213–214.CrossRefGoogle Scholar
  51. Mandel, A. D., K. Wright, and J. M. McKinnon. 1964. Selective medium for the isolation of Mima and Herellae organisms. J. Bacteriol. 88: 1524–1525.PubMedPubMedCentralGoogle Scholar
  52. Marcus, B. B., S. B. Samuels, B. Pittman, and W. B. Cherry. 1969. A serologic study of Herellea vaginicola and its identification by immunofluorescent staining. Amer. J. Clin. Pathol. 52: 309–319.Google Scholar
  53. Mukherji, S. J. and N. Bhopale. 1983. Gliding motility of Acinetobacter anitratus. J. Clin. Pathol. 36: 484.Google Scholar
  54. Noble, W. C. and D. G. Pitcher. 1978. Microbial ecology of the human skin. Adv. Microbial Ecol. 2: 245–289.CrossRefGoogle Scholar
  55. Oberhofer, T. R. 1979. Comparison of the API 20E and OxiFerm systems in identification of nonfermentative and oxidase-positive fermentative bacteria. J. Clin. Microbiol. 9: 220–226.PubMedPubMedCentralGoogle Scholar
  56. Oberhofer, T. R., J. W. Rowen, and G. E Cunningham. 1977. Characterisation and identification of Gram-negative nonfermentative bacteria. J. Clin. Microbiol. 5: 208–220.PubMedPubMedCentralGoogle Scholar
  57. Ovrebo, S. and K. Kleppe. 1973. Pyrimidine metabolism in Acinetobacter calcoaceticus. J. Bacteriol. 116: 331–336.PubMedPubMedCentralGoogle Scholar
  58. Pines, O. and D. Gutnick. 1986. Role for emulsan in growth of Acinetobacter calcoaceticus RAG-1 on crude oil. Appl. Environ. Microbiol. 51: 661–663.Google Scholar
  59. Rosenberg, E., A. Parry, D. Gibson, and D. L. Gutnick. 1979a. Emulsifier of Arthrobacter RAG-l: specificity of hydrocarbon substrate. Appl. Environ. Microbiol. 37: 409–413.PubMedPubMedCentralGoogle Scholar
  60. Rosenberg, E., C. Rubinovitz, A. Gottlieb, S. Rosenhak, and E. Z. Ron. 1988a. Production of biodispersan by Acinetobacter calcoaceticus A2. Appl. Environ. Microbiol. 54: 317–322.PubMedPubMedCentralGoogle Scholar
  61. Rosenberg, E., C. Rubinovitz, R. Legmann, and E. Z. Ron. 1988b. Purification and chemical properties of Acinetobacter calcoaceticus A2 biodispersan. Appl. Environ. Microbiol. 54: 323–326.PubMedPubMedCentralGoogle Scholar
  62. Rosenberg, E., A. Zuckerberg, C. Rubinovitz, and D. L. Gutnick. 1979b. Emulsifier of Arthrobacter RAG-1: isolation and emulsifying properties. Appl. Environ. Microbiol. 37: 402–408.PubMedPubMedCentralGoogle Scholar
  63. Sawula, R. V. and I. P. Crawford. 1972. Mapping of the tryptophan genes of Acinetobacter calcoaceticus by transformation. J. Bacteriol. 112: 797–805.PubMedPubMedCentralGoogle Scholar
  64. Shabtai, Y., O. Pines, and D. L. Gutnick. 1985. Emulsan: a case study of microbial capsules as industrial products. Dev. Ind. Microbiol. 26: 291–307.Google Scholar
  65. Sherertz, R. G. and M. L. Sullivan. 1985. An outbreak of infections with Acinetobacter calcoaceticus in burn patients: contamination of patients’ mattresses. J. Infect. Dis. 151: 252–258.Google Scholar
  66. Shewan, J. W., G. Hobbs, and W. Hodgkiss. 1960. The Pseudomonas and Achromobacter groups of bacteria in the spoilage of marine white fish. J. Appl. Bacteriol. 23: 463–468.CrossRefGoogle Scholar
  67. Singer, J. T., J. J. van Tuijl, and W. R. Finnerty. 1986. Transformation and mobilization of cloning vectors in Acinetobacter spp. J. Bacteriol 165: 301–303.PubMedPubMedCentralGoogle Scholar
  68. Smith, R. W. and R. M. Massanari. 1977. Room humidifiers as the source of Acinetobacter infections. J. Amer. Med. Assoc. 237: 995–997.Google Scholar
  69. Taplin, D., G. Robell and N. Zaias. 1963. The human skin as a source of Mima-Herellea infection. J. Amer. Med. Assoc. 186: 952–959.Google Scholar
  70. Taylor, W. H. and E. Juni. 1961. Pathways for biosynthesis of bacterial capsular polysaccharide. J. Bacteriol. 81: 694–703.PubMedPubMedCentralGoogle Scholar
  71. Thornley, M. J., M. Ingram, and E. M. Barnes. 1960. The effects of antibiotics and irradiation on the Pseudomonas-Achromobacter flora of chilled poultry. J. Appl. Bacteriol. 23: 487–498.CrossRefGoogle Scholar
  72. Towner, K. J. 1978. Chromosome mapping in Acinetobacter calcoaceticus. J. Gen. Microbiol. 104: 175–180.PubMedCrossRefGoogle Scholar
  73. Towner, K. J. and B. A. Chopade. 1987. Biotyping of Acinetobacter calcoaceticus using the API 20NE system. J. Hosp. Infect. 10: 145–151.PubMedCrossRefGoogle Scholar
  74. Towner, K. J. and A. Vivian. 1976a. RP4-mediated conjugation in Acinetobacter calcoaceticus. J. Gen. Microbiol. 93: 355–360.PubMedCrossRefGoogle Scholar
  75. Towner, K. J. and A. Vivian. 1976b. RP4 fertility variants in Acinetobacter calcoaceticus. Genet. Res. 28: 301–306.PubMedCrossRefGoogle Scholar
  76. Towner, K. J. and A. Vivian. 1977. Plasmids capable of transfer and chromosome mobilization in Acinetobacter calcoaceticus. J. Gen. Microbiol. 101: 167–171.CrossRefGoogle Scholar
  77. Twarog, R. and L. E. Blouse. 1968. Isolation and characterization of transducing bacteriophage BP1 for Bacterium anitratum (Achromobacter sp.). J. Virol. 2: 716–722.PubMedPubMedCentralGoogle Scholar
  78. Vakeria, D., A. Vivian, and C. A. Fewson. 1984. Isolation, characterization and mapping of mandelate pathway mutants of Acinetobacter calcoaceticus. J. Gen. Microbiol. 130: 2893–2903.PubMedGoogle Scholar
  79. Warskow, A. L. and E. Juni. 1972. Nutritional requirements of Acinetobacter strains isolated from soil, water and sewage. J. Bacteriol. 112: 1014–1016.PubMedPubMedCentralGoogle Scholar
  80. Whittaker, P. A. 1971. Terminal respiration in Moraxella lwoffs (NCIB 8250). Microbios 4: 65–70.PubMedGoogle Scholar
  81. Zuckerberg, A., A. Diver, Z. Peeri, D. L. Gutnick, and E. Rosenberg. 1979. Emulsifier of Arthrobacter RAG-1: chemical and physical properties. Appl. Environ. Microbiol. 37: 414–420.Google Scholar

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  • Kevin J. Towner

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