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Three-Dimensional Reconstruction of Noncrystalline Macromolecular Assemblies

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Electron Tomography

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Abstract

At present the only method available for determining the 3D molecular structure of most biological macromolecules is x-ray crystallography. However, some macro-molecules associate to form large multisubunit complexes (e.g., viruses, ribosomes, multisubunit enzymes) for which crystals suitable for crystallographic studies are not easily obtained. Even in those cases where suitable crystals are available, there remain additional technical problems that will have to be solved before detailed structures can be determined (Yonath and Wittman, 1989). In this chapter I will describe recent structural determinations of noncrystalline biomacromolecular complexes by the technique of electron microscopy in conjunction with 3D recon-struction (electron tomography).

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Authors and Affiliations

  1. Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York, 12201-0509, USA

    Terence Wagenknecht

  2. Department of Biomedical Sciences, School of Public Health, State University of New York at Albany, Albany, New York, 12222, USA

    Terence Wagenknecht

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  1. Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York, USA

    Joachim Frank

  2. Department of Biomedical Sciences, School of Public Health, State University of New York at Albany, Albany, New York, USA

    Joachim Frank

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Wagenknecht, T. (1992). Three-Dimensional Reconstruction of Noncrystalline Macromolecular Assemblies. In: Frank, J. (eds) Electron Tomography. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-2163-8_15

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