Skin Pharmacology and Dermatology

  • Andreas J. Bircher
  • Richard H. Guy
  • Howard I. Maibach
Part of the Developments in Cardiovascular Medicine book series (DICM, volume 107)

Abstract

Laser Doppler flowmetry (LDF) has many uses in pharmacologic dermatology. The topics covered in this chapter include cutaneous pharmacology and pharmacodynamics of vasoactive compounds, determination of transcutaneous drug penetration kinetics, and evaluation of the potency of penetration enhancers. Ultraviolet erythema, the effects of inflammatory mediators, irritation from chemical compounds, and patch-test allergens have been studied. Newer applications are the objective measurement of skin lesions, the control of dermatologic therapy, and comparison of the reactivity of the cutaneous microcirculation in different age and ethnic groups. This chapter presents our experience with LDF in the dermatologic and pharmacological investigations and reviews related publications.

Keywords

Contact Dermatitis Laser Doppler Flowmetry Skin Blood Flow Laser Doppler Velocimetry Percutaneous Absorption 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Rovensky, J., and A. Preis. 1959. The significance of the trafuril test, with special reference to atopy and rheumatic fever. A capillaroscopic study. Ann Paediat 193:289–303.PubMedGoogle Scholar
  2. 2.
    Murell, T.W. Jr., and W.M. Taylor. 1959. The cutaneous reaction to nicotinic acid furfuryl. Arch Dermatol Syph 79:545–552.CrossRefGoogle Scholar
  3. 3.
    Stoughton, R.B., W.E. Clendenning, and D. Kruse. 1960. Percutaneous absorption of nicotinic acid and derivatives. J Invest Dermatol 35:337–341.Google Scholar
  4. 4.
    Fountain, R. B., B.S. Baker, J.W. Hadgraft, and I. Sarkany. 1969. The rate of absorption and duration of action of four different solutions of methyl nicotinate. Br J Dermatol 81: 202–206.PubMedCrossRefGoogle Scholar
  5. 5.
    Albery, W.J., and J. Hadgraft. 1979. Percutaneous absorption: theoretical description. J Pharm Pharmacol 31:129–139.PubMedCrossRefGoogle Scholar
  6. 6.
    Albery, W.J., and J. Hadgraft. 1979. Percutaneous absorption, in vivo experiments. J Pharm Pharmacol 31:140–147.PubMedCrossRefGoogle Scholar
  7. 7.
    Fulton, G. P., E. M. Farber, and A.P. Moreci. 1959. The mechanism of action of rubefacients. J Invest Dermatol 33:317–325.PubMedCrossRefGoogle Scholar
  8. 8.
    Guy, R.H., and H.I. Maibach. 1982. Rapid radial transport of methyl nicotinate in the dermis. Arch Dermatol Res 273:91–95.PubMedCrossRefGoogle Scholar
  9. 9.
    Albery, W.J., R.H. Guy, and J. Hadgraft. 1983. Percutaneous absorption: transport in the dermis. Int J. Pharm 15:125–148.CrossRefGoogle Scholar
  10. 10.
    Svedmyr, N., A. Heggelund, and G. Åberg. 1979. Influence of indomethacin on flush induced by nicotinic acid in man. Acta Pharmacol Toxicol 41:397–400.CrossRefGoogle Scholar
  11. 11.
    Wilkin, J.K., O. Wilkin, R. Kapp, R. Donachie, M. E. Chernovsky, and J. Buckner. 1982. Aspirin blocks nicotinic acid-induced flushing. Clin Pharmacol Ther 31:478–482.PubMedCrossRefGoogle Scholar
  12. 12.
    Wilkin, J.K., G. Fortner, L.A. Reinhardt, O. V. Flowers, S.J. Kilpatrick, and W.C. Streeter. 1985. Prostaglandins and nicotinate-provoked increase in cutaneous blood flow. Clin Pharmacol Ther 38:273–277.PubMedCrossRefGoogle Scholar
  13. 13.
    Lahti, A., A. Väänänen, E.-L. Kokkonen, and M. Hannuksela. 1987. Acetylsalicylic acid inhibits non-immunologic contact urticaria. Contact Dermatitis 16:133–135.PubMedCrossRefGoogle Scholar
  14. 14.
    Lahti, A. 1987. Terfenadine does not inhibit non-immunologic contact urticaria. Contact Dermatitis 16:220–223.PubMedCrossRefGoogle Scholar
  15. 15.
    Guy, R. H., E. Tur, R.C. Wester, and H. I. Maibach. 1985. Blood flow studies and percutaneous absorption. In Percutaneous Absorption, Bronaugh, R.L., Maibach, H.I., eds New York: Marcel Dekker, 393–407.Google Scholar
  16. 16.
    Guy, R. H., R.C. Wester, E. Tur, and H. I. Maibach. 1983. Noninvasive assessments of the percutaneous absorption of nicotinate in humans. J Pharm Sci 72:1077–1079.PubMedCrossRefGoogle Scholar
  17. 17.
    Guy, R. H., E. Bugatto, B. Tur, C. Gaebel, L. B. Sheiner, and H. I. Maibach. 1984. Pharmacodynamic measurements of methyl nicotinate percutaneous absorption. Pharm Res 1:76–81.CrossRefGoogle Scholar
  18. 18.
    Tenland, T., E.G. Salerud, G.E. Nilsson, and P. Å. Öberg. 1983. Spatial and temporal variations in human skin blood flow. Int J Microcirc Clin Exp 2:81–90.PubMedGoogle Scholar
  19. 19.
    Sundberg, S. 1984. Acute effects and long-term variations in skin blood flow measured with laser Doppler flowmetry. Scand J Clin Lab Invest 44:341–345.PubMedCrossRefGoogle Scholar
  20. 20.
    Tur, E., M. Tur, H.I. Maibach, and R.H. Guy. 1983. Basal perfusion of the cutaneous microcirculation: measurements as a function of anatomic position. J Invest Dermatol 81:442–446.PubMedCrossRefGoogle Scholar
  21. 21.
    Dowd, P. M., M. Whitefield, and M. W. Greaves. 1987. Hexyl nicotinate-induced vasodilation in normal human skin. Dermatologica 174:239–243.PubMedCrossRefGoogle Scholar
  22. 22.
    Tur, E., H.I. Maibach, and R.H. Guy. 1985. Spatial variability of vasodilation in human forearm skin. Br J Dermatol 113:197–203.PubMedCrossRefGoogle Scholar
  23. 23.
    Müller, P., R. Keller, and P. Imhof. 1987. Laser Doppler flowmetry, a reliable technique for measuring pharmacologically induced changes in cutaneous blood flow? Methods Find Exp Clin Pharmacol 9:409–420.PubMedGoogle Scholar
  24. 24.
    De Boer, E. M., P. D. Bezemer, and D.P. Bruynzeel. A standard method for repeated recording of skin blood low using laser Doppler flowmetry. Dermatosen in press.Google Scholar
  25. 25.
    Guy, R. H., E. M. Carlstrøm, D. A. W. Bucks, R.S. Hinz, and H. I. Maibach. 1986. Percutaneous penetration of nicotinates: in vivo and in vitro measurements. J Pharm Sci 75:968–972.PubMedCrossRefGoogle Scholar
  26. 26.
    Ryatt, K. S., J.M. Stevenson, H. I. Maibach, and R. H. Guy. 1986. Pharmacodynamic measurement of percutaneous penetration enhancement in vivo. J Pharm Sci 75:374–377.PubMedCrossRefGoogle Scholar
  27. 27.
    Kohli, R., W.I. Archer, and A. Li Wan Po. 1987. Laser velocimetry for the non-invasive assessment of the percutaneous absorption of nicotinates. Int J Pharm 36:91–98.CrossRefGoogle Scholar
  28. 28.
    Bircher, A.J., K. V. Roskos, H.I. Maibach, and R.H. Guy. 1980. Laser Doppler veløcimetric measurement of skin blood flow: a reply. Int J Pharm 45:263–265.CrossRefGoogle Scholar
  29. 29.
    Wester, R. C., H. I. Maibach, R. H. Guy, and E. Novak. 1984. Minoxidil stimulates cutaneous blood flow in human balding scalps: pharmacodynamics measured by laser Doppler velocimetry and photopulse plethysmography. J Invest Dermatol 82:515–517.PubMedCrossRefGoogle Scholar
  30. 30.
    Wahlberg, J. E. 1983. Skin irritancy from alkaline solutions assessed by laser Doppler flowmetry. Contact Dermatitis 10:111.CrossRefGoogle Scholar
  31. 31.
    Wahlberg, J. E. 1984. Erythema-inducing effects of solvents following epicutaneous administration to man studied by laser Doppler flowmetry. Scand J Work Environ Health 10:159–162.PubMedCrossRefGoogle Scholar
  32. 32.
    Wahlberg, J.E., and G. Nilsson. 1984. Skin irritancy from propylenglycol. Acta Derm Venereol 64:286–290.PubMedGoogle Scholar
  33. 33.
    Nilsson, G.E., U. Otto, and J.E. Wahlberg. 1982. Assessment of skin irritancy in man by laser Doppler flowmetry. Contact Dermatitis 8:401–406.PubMedCrossRefGoogle Scholar
  34. 34.
    Blanken, R., P. G. M. van der Valk, and J. P. Nater. 1986. Laser-Doppler flowmetry in the investigation of irritant compounds on human skin. Dermatosen 34:5–9.Google Scholar
  35. 35.
    Gilchrest B.A. 1984. Skin and Aging Processes. Boca Raton, FL:CRC Press Inc.Google Scholar
  36. 36.
    Roskos, K. V., R. H. Guy, and Maibach H. I. 1986. Percutaneous absorption in the aged. Dermatol Clin 4:455–465.PubMedGoogle Scholar
  37. 37.
    Skassa-Brociek, W., J-C Manderscheid, F.B. Michel, and J. Bousquet. 1987. Skin test reactivity to histamine from infancy to old age. J Allergy Clin Immunol 80:711–716.PubMedCrossRefGoogle Scholar
  38. 38.
    Guy, R.H., E. Tur, S. Bjerke, and H.I. Maibach. 1985. Are there age and racial differences to methyl nicotinate-induced vasodilation in human skin? J Am Acad Dermatol 12:1001–1006.PubMedCrossRefGoogle Scholar
  39. 39.
    Roskos, K. V., A.J. Bircher, H.I. Maibach, and R.H. Guy. 1989. A laser Doppler velocimetric investigation of the cutaneous microcirculation as a function of age. Submitted.Google Scholar
  40. 40.
    Gean, C.J., E. Tur, H. I. Maibach, and R. H. Guy. Cutaneous responses to topical methyl nicotinate in black, oriental and caucasian skin. Arch Derm Res 281:95–98.Google Scholar
  41. 41.
    Bunker, C. B., and P.M. Dowd. 1988. Alterations in scalp blood flow after the epicutaneous application of 3% minoxidil and 0.1% hexyl nicotinate in alopecia. Br J Dermatol 117:668–669.CrossRefGoogle Scholar
  42. 42.
    Stevenson, J. M., H. I. Maibach, and R. H. Guy. 1987. Laser Doppler and photoplethysmographic assessment of cutaneous microvasculature. In Models in Dermatology, Maibach, H. I., Lowe, N.L. eds. New York:Karger, vol. 3, pp 121–140.Google Scholar
  43. 43.
    Sundberg, S., and M. Castrén. 1986. Drug- and temperature-induced changes in peripheral circulation measured by laser-Doppler flowmetry and digital-pulse plethysmography. Scand J Clin Lab Invest 46:359–365.PubMedCrossRefGoogle Scholar
  44. 44.
    McKenzie, A. W., and R. B. Stoughton. 1962. Method for comparing percutaneous absorption of steroids. Arch Dermatol 86:608–611.CrossRefGoogle Scholar
  45. 45.
    Amantea, M., E. Tur, H.I. Maibach, and R.H. Guy. 1983. Preliminary skin blood flow measurements appear unsuccessful for assessing topical corticosteroid effect. Arch Dermatol Res 275:419–420.PubMedCrossRefGoogle Scholar
  46. 46.
    Bisgaard, H., J.K. Kristensen, and J. Søndergaard. 1986. A new technique for ranking vascular corticosteroid effects in humans using laser-Doppler velocimetry. J Invest Dermatol 86:275–278.PubMedCrossRefGoogle Scholar
  47. 47.
    Serup, J., and P. Holm. 1985. Domoprednate (stermonid®), a topical D-homocorticosteroid, skin atrophy and telangiectasia. Dermatologica 170:189–194.PubMedCrossRefGoogle Scholar
  48. 48.
    Serup, J., P. Holm, I.-M. Stender, and J. Pichard. 1987. Skin atrophy and teleangiectasia after topical corticosteroids as measured non-invasively with high frequency ultrasound, evaporimetry and laser Doppler flowmetry. Methodological aspects including evaluations of regional differences. Bioeng Skin 3:42–48.Google Scholar
  49. 49.
    Holloway, GA. 1980. Cutaneous blood flow responses to injection trauma measured by laser Doppler velocimetry. J Invest Dermatol 74:1–4.PubMedCrossRefGoogle Scholar
  50. 50.
    Serup, J., and B. Staberg. 1985. Quantification of weal reactions with laser Doppler flowmetry. Allergy 40:233–237.PubMedCrossRefGoogle Scholar
  51. 51.
    Hovell, C.J., C. R. W. Beasley, R. Mani, and S. T. Holgate. 1987. Laser Doppler flowmetry for determining changes in cutaneous blood flow following intradermal injection of histamine. Clin Allergy 17:469–479.PubMedCrossRefGoogle Scholar
  52. 52.
    Bisgaard, H., J. Kristensen, and J. Søndergaard. 1982. The effect of leucotriene C4 and D4 on cutaneous blood flow in humans. Prostaglandins 23:797–801.PubMedCrossRefGoogle Scholar
  53. 53.
    Bisgaard, H., and J.K. Kristensen. 1984. Quantitation of microcirculatory blood flow changes in human cutaneous tissue induced by inflammatory mediators. J Invest Dermatol 83:184–187.PubMedCrossRefGoogle Scholar
  54. 54.
    Bisgaard, H. 1987. Vascular effects of leucotriene D4 in human skin. J Invest Dermatol 88:109–114.PubMedCrossRefGoogle Scholar
  55. 55.
    Chan, C., and A. Ford-Hutchinson. 1985. Effects of synthetic leukotrienes on local skin blood flow and vascular permeability in porcine skin. J Invest Dermatol 84:154–157.PubMedCrossRefGoogle Scholar
  56. 56.
    Knight, K. R., P. A. Collopy, and B. M. O’Brien. 1986. Response of the rabbit cutaneous microcirculation to prostanoid modifiers as determined by laser Doppler flowmetry. Microvasc Res 31:197–202.PubMedCrossRefGoogle Scholar
  57. 57.
    Maurice, P. D. L., R.M. Barr, O. Koro, and M. W. Greaves. 1987. The effect of prostaglandin D2 on the response of human skin to histamine. J Invest Dermatol 89:245–248.PubMedCrossRefGoogle Scholar
  58. 58.
    Lindblad, L. E., L. Ekenvall, K. Ancker, H. Rohman, and P. Å Oberg. 1987. Laser Doppler flowmeter assessment of iontophoretically applied norepinephrine on human finger skin circulation. J Invest Dermatol 87:634–636.CrossRefGoogle Scholar
  59. 59.
    Lindblad, L.E., and L. Ekenvall. 1986. Alpha-adrenoceptors in the vessels of human finger skin. Acta Physiol Scand 128:219–222.PubMedCrossRefGoogle Scholar
  60. 60.
    Brain, S.D., J. R. Tippins, H. R. Morris, I. Maclntyre, and T.J. Williams. 1986. Potent vasodilator activity of calcitonin gene-related peptide in human skin. J Invest Dermatol 87:533–536.PubMedCrossRefGoogle Scholar
  61. 61.
    Berardesca, E., and H.I. Maibach. 1988. Bioengineering and the patch test. Contact Dermatitis 12:3–9.CrossRefGoogle Scholar
  62. 62.
    Willis, C. M., C.J.M. Stephens, and J.D. Wilkinson. 1988. Assessment of erythema in irritant contact dermatitis. Contact Dermatitis 18:138–142.PubMedCrossRefGoogle Scholar
  63. 63.
    Frödin, T., and C. Anderson. 1987. Multiple parameter assessment of skin irritancy. Contact Dermatitis 17:92–99.PubMedCrossRefGoogle Scholar
  64. 64.
    Frödin, T., M. Skogh, and L. Molin. 1988. The importance of the coal tar bath in the Ingram treatment of psoriasis. Evaluation by evaporimetry and laser Doppler flowmetry. Br J Dermatol 118:429–434.PubMedCrossRefGoogle Scholar
  65. 65.
    Wulf, H. C., B. Staberg, and W.H. Eriksen. 1983. Laser Doppler measurements of blood flow in pig skin after sunscreens and artificial light. Photochem Photobiophys 6:231–237.Google Scholar
  66. 66.
    Wahlberg, J.E., and E. Wahlberg. 1984. Patch test irritancy quantified by laser Doppler flowmetry. Contact Dermatitis 11:257–258.PubMedCrossRefGoogle Scholar
  67. 67.
    Wahlberg, J.E., and E. Wahlberg. 1985. Skin irritancy from nickel sulfate and test patches. Contact Dermatitis 13:224–225.PubMedCrossRefGoogle Scholar
  68. 68.
    Staberg, B., P. Klemp, and J. Serup. 1984. Patch test responses evaluated by cutaneous blood flow measurements. Arch Dermatol 120:741–743.PubMedCrossRefGoogle Scholar
  69. 69.
    Wahlberg, J. E., and E. N. G. Wahlberg. 1987. Quantification of skin blood flow at patch test sites. Contact Dermatitis 17:229–233.PubMedCrossRefGoogle Scholar
  70. 70.
    Staberg, B., and Serup, J. 1988. Allergic and irritant skin reactions evaluated by laser Doppler flowmetry. Contact Dermatitis 18:40–45.PubMedCrossRefGoogle Scholar
  71. 71.
    Andersen, K.E., and B. Staberg. 1985. Quantitation of contact allergy in guinea pigs by measuring changes in skin blood flow and skin fold thickness. Acta Derm Venereol 65:37–42.PubMedGoogle Scholar
  72. 72.
    Lachapelle, J.M., M. Mahmoud, and R. Vanherle. 1984. Anhydrite reaction in coal mines. Contact Dermatitis 11:188–189.PubMedCrossRefGoogle Scholar
  73. 73.
    Serup, J., and E. Ploug. 1987. Testning af sanitetsrengøringsmidler for hudirritation. Ugeskr Laeger 149:472–473.PubMedGoogle Scholar
  74. 74.
    Mahmoud, M., and J.M. Lachapelle. 1985. Evaluation of the protective value of an antisolvent gel by laser Doppler flowmetry and histology. Contact Dermatitis 13:14–19.PubMedCrossRefGoogle Scholar
  75. 75.
    Blanken, R., P. G. M. van der Valk, J.P. Nater, and H. Dijkstra. 1987. After-work emollient creams: effects on irritant skin reactions. Dermatosen 35:95–98.Google Scholar
  76. 76.
    Van Neste, D., M. Masmoudi, G. Mahmoud, and J.M. Lachapelle. 1986. Regression patterns of transepidermal water loss and of cutaneous blood flow values in sodium lauryl sulfate induced irritation: a human model of rough dermatitic skin. Bioeng Skin 2:103–118.Google Scholar
  77. 77.
    Van Neste, D., G. Mahmoud, and M. Masmoudi. 1987. Experimental induction of rough dermatitic skin in humans. Contact Dermatitis 16:27–33.PubMedCrossRefGoogle Scholar
  78. 78.
    Weigand, D. A., and G.R. Gaylor. 1974. Irritant reaction in Negro and Caucasian skin. South Med J 67:548–551.PubMedCrossRefGoogle Scholar
  79. 79.
    Berardesca, E., and H.I. Maibach. 1988. Racial differences in sodium lauryl sulphate induced cutaneous irritation: black and white. Contact Dermatitis 18:65–70.PubMedCrossRefGoogle Scholar
  80. 80.
    Lammintausta, K., H.I. Maibach, and D. Wilson. 1988. Human cutaneous irritation: induced hyporeactivity. Contact Dermatitis 17:193–198.CrossRefGoogle Scholar
  81. 81.
    Lammintausta, K., H.I. Maibach, and D. Wilson. 1987. Irritant reactivity in males and females. Contact Dermatitis 17:276–280.PubMedCrossRefGoogle Scholar
  82. 82.
    Blichmann, C., and J. Serup. 1987. Hydration studies on scaly hand eczema. Contact Dermatitis 16:155–159.PubMedCrossRefGoogle Scholar
  83. 83.
    Stern, M., D. L. Lappe, P. D. Bowen, J. E. Chimosky, G. A. Holloway, H. R. Keiser, and R.L. Bowman. 1977. Continuous measurement of tissue blood flow by laser Doppler spectroscopy. Am J Physiol 232:H441–H448.PubMedGoogle Scholar
  84. 84.
    Holloway, G. A., and D.W. Watkins. 1977. Laser Doppler measurement of cutaneous blood flow. J Invest Dermatol 69:306–309.PubMedCrossRefGoogle Scholar
  85. 85.
    Watkins, D. W., and G. A. Holloway. 1978. An instrument to measure cutaneous blood flow using the Doppler shift of laser light. IEEE Trans Biomed Eng 25:28–33.PubMedCrossRefGoogle Scholar
  86. 86.
    Drouard, V., D. R. Wilson, HI. Maibach, and R. H. Guy. 1984. Quantitative assessment of UV-induced changes in microcirculatory flow by laser Doppler velocimetry. J Invest Dermatol 83:188–192.PubMedCrossRefGoogle Scholar
  87. 87.
    Young, A.R., R.H. Guy, and H.I. Maibach. 1985. Laser Doppler velocimetry to quantify UV-B induced increase in human skin blood flow. Photochem Photobiol 42:385–390.PubMedCrossRefGoogle Scholar
  88. 88.
    Farr, P. M., and B.L. Diffey. 1984. Quantitative studies on cutaneous erythema induced by ultraviolet radiation. Br J Dermatol 111:673–682.PubMedCrossRefGoogle Scholar
  89. 89.
    Wan, S., K. F. Jaenicke, and J. A. Parrish. 1983. Comparison of the erythemogenic effectiveness of ultraviolet-B (290–320 nm) and ultraviolet-A (320–400 nm) radiation by skin reflectance. Photochem Photobiol 37:547–552.PubMedCrossRefGoogle Scholar
  90. 90.
    Farr, P. M., and B.L. Diffey. 1986. The vascular response of human skin to ultraviolet radiation. Photochem Photobiol 44:501–507.PubMedCrossRefGoogle Scholar
  91. 91.
    Diffey, B. L., P.M. Farr, and A. M. Oakley. 1987. Quantitative studies on UVA-induced erythema in human skin. Br J Dermatol 117:57–66.PubMedCrossRefGoogle Scholar
  92. 92.
    Serup, J., and J.K. Kristensen. 1984. Blood flow of morphoea plaques as measured by laser Doppler flowmetry. Arch Dermatol Res 276:322–325.PubMedCrossRefGoogle Scholar
  93. 93.
    Jünger, M., B. Rahmel, G. Rassner, and C. Scherwitz. 1988. Laser-Doppler-Flux zur Therapie kontrolle bei Acrodermatitis chronica atrophicans. Phlebol Proctol 17:136–139.Google Scholar
  94. 94.
    Zackheim, H. S., E. Tur, and R.H. Guy. 1985. Regional blood flow and mycosis fungoides. J Am Acad Dermatol 12:578–580.PubMedCrossRefGoogle Scholar
  95. 95.
    Wilkin, J.K., and G. Fortner. 1985. Cutaneous vascular sensitivity to lower aliphatic alcohols and aldehydes in orientals. Alcohol Clin Exp Res 9:522–525.PubMedCrossRefGoogle Scholar
  96. 96.
    Wilkin, J.K. 1986. Quantitative assessment of alcohol-provoked flushing. Arch Dermatol 122:63–65.PubMedCrossRefGoogle Scholar
  97. 97.
    Wilkin, J.K. 1986. Does monosodium glutamate cause flushing (or merely “glutamania”)? J Am Acad Dermatol 15:225–230.PubMedCrossRefGoogle Scholar
  98. 98.
    Ek, A.-C, D.H. Lewis, H. Zetterqvist, and P.-G. Svensson. 1984. Skin blood flow in an area at risk for pressure sores. Scand J Rehabil Med 16:85–89.PubMedGoogle Scholar
  99. 99.
    Ek, A.-C., G. Gustavsson, and D.H. Lewis. 1985. The local skin blood flow in areas at risk for pressure sores treated with massage. Scand J Rehabil Med 17:81–86.PubMedGoogle Scholar
  100. 100.
    Karlsmark, T., and J.K. Kristensen. 1987. A method for testing the effect of pressurerelieving materials in the prevention of pressure ulcers. Acta Derm Venereol 67:260–263.PubMedGoogle Scholar
  101. 101.
    Klemp, P. 1987. Cutaneous and subcutaneous blood flow measurements in psoriasis. Dan Med Bull 34:147–159.PubMedGoogle Scholar
  102. 102.
    Staberg, B., and P. Klemp. 1984. Skin blood flow in psoriasis during Goeckerman or beech tar therapy. Acta Derm Venereol 64:331–334.PubMedGoogle Scholar
  103. 103.
    Klemp, P., and B. Staberg. 1985. The effect of antipsoriatic treatment on cutaneous blood flow in psoriasis measured by 133Xenon washout method and laser Doppler velocimetry. J Invest Dermatol 85:259–263.PubMedCrossRefGoogle Scholar
  104. 104.
    Khan, A., L.M. Schall, E. Tur, H. I. Maibach, and R. H. Guy. 1987. Blood flow in psoriatic skin lesions: the effect of treatment. Br J Dermatol 117:193–201.PubMedCrossRefGoogle Scholar
  105. 105.
    Marks, R., D. Black, A.D. Pearse, and S. Hill. 1986. Techniques for assessing the activity of topically applied retinoids. J Am Acad Dermatol 15:810–816.PubMedCrossRefGoogle Scholar
  106. 106.
    Kligman, A.L., G.L. Grove, R. Hirose, and J.J. Leyden. 1986. Topical tretinoin for photoaged skin. J Am Acad Dermatol 15:836–859.PubMedCrossRefGoogle Scholar
  107. 107.
    Schiltz, J. R., J. Lanigan, W. Nabial, B. Petty, and J. E. Birnbaum. 1986. Retinoic acid induces cyclic changes in epidermal thickness and dermal collagen and glycosaminglycan biosynthesis rates. J Invest Dermatol 87:663–667.PubMedCrossRefGoogle Scholar
  108. 108.
    Grove, G. L., M.J. Grove, C. R. Zerweck, and J.J. Leyden. 1988. Computerized laser Doppler velocimetry assessment of cutaneous microcirculation: effects of topical tretinoin treatment in photoaged skin. J Cut Aging Cosm Dermatol 1:27–32.Google Scholar
  109. 109.
    Schnizer, W., R. Erdl, P. Schöps, and N. Seichert. 1985. The effects of external CO2 application on human skin microcirculation investigated by laser Doppler flowmetry. Int J Microcirc Clin Exp 4:343–350.PubMedGoogle Scholar
  110. 110.
    Lanigan, S.W., and J.A. Cotterill. 1987. Objective assessments of portwine stains. Br J Dermatol 117:33–34.CrossRefGoogle Scholar
  111. 111.
    Swanson Beck, J., and V.A. Spence. 1986. Patterns of blood flow in the microcirculation of the skin during the course of the tuberculin reaction in normal human subjects. Immunology 58:209–215.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Andreas J. Bircher
  • Richard H. Guy
  • Howard I. Maibach

There are no affiliations available

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