The Arachidonic Acid Signal System in the Thyroid: Regulation by Thyrotropin and Insulin/IGF-I
Previous reports (1–11) have defined the importance of the Ca/phosphoinositide/arachidonic acid signal system to both the function and growth of FRTL-5 rat thyroid cells and to the action of both thyrotropin (TSH) and alpha-1 adrenergic agents in these cells. Thus evidence has been presented that norepinephrine and TSH could increase degradation of phosphatidylinositol 4,5-bisphosphate (PIP2) (1) with the concomitant formation of diacyiglycerol and IP3 (2). This action was accompanied by increases in cytosolic Ca++ (3), arachidonic acid release from the cells (4, 5) and the action of arachidonic acid metabolites in processes important to thyroid hormone formation and growth (4–11).
KeywordsArachidonic Acid Pertussis Toxin Thyroid Cell Arachidonic Acid Metabolite Arachidonic Acid Release
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- 9.P. Santisteban, M. DeLuca, D. Corda, E.F. Grollman and L. D. Kohn, Regulation of thyroglobulin iodination and thyroid hormone formation in FRTL-5 thyroid cells, in: “Fronteirs in Thyroidology, v. 2,” G. Medeiros-Neto and E. Gaitan, Plenum Press, New York (1986).Google Scholar
- 10.L. D. Kohn, E. A. Bone, J. Y. Chan, D. Corda, O. Isozaki, A. Luini, C.Marcocci, P. Santisteban and E. F. Grollman, Interactions of peptinergic hormone and biogenic amine signals in the regulation of thyroid function and growth, in: “Tansduction of Neuronal Signals,” P. J. Magistretti, J. H. Morrison and T.D. Reisine, eds., Foundation for the study of the Nervous system, Geneva (1986).Google Scholar
- 11.J. Axelrod, Transducing mechanism in pituitary, thyroid and visual system, in: “Tansduction of Neuronal Signals,” P. J. Magistretti, J. H. Morrison and T.D. Reisine, eds., Foundation for the study of the Nervous system, Geneva (1986).Google Scholar
- 14.L. D. Kohn, E. Yavin, Z. Yavin, P. Laccetti, P. Vitti, E. F. Grollman and W. A. Valente, Autoimmune thyroid disease studied with monoclonal antibodies to the TSH receptor, in: “Monoclonal Antibodies: Probes for the Study of Autoimmunity and Immunodeficiency,” B. F. Haynes and G. S. Eisenbarth, eds., Academic Press, New York (1983).Google Scholar
- 15.L. D. Kohn, D. Tombaccini, M. De Luca, M. Bifulco, E. F. Grollman and W. A. Valente, Monoclonal antibodies and the thyrotropin receptor, in: “Monoclonal Antibodies to Receptors: Probes for Receptor Structure and Function,” M. F. Greaves, ed., Receptors and Recognition, Series B, 17: 201 (1984).Google Scholar
- 16.L. D. Kohn, F. V. Alvarez, C. Marcocci, A. D. Kohn, A. Chen, W. E. Hoffman, D. Tombaccini, W. A. Valente, M. De Luca, P. Santisteban and E. F. Grollman, Monoclonal antibody studies defining the origin and properties of Graves’ autoantibodies, Ann. N. Y. Acad. Sci., 475: 157 (1986).PubMedCrossRefGoogle Scholar
- 17.J. Y. Chan, M. De Luca, P. Santisteban, O. Isozaki, S. Shifrin, S. M. Aloj, E. F. Grollman and L. D. Kohn, Nature of thyroid autoantigens: the TSH receptor, in: Thyroid Autoimmunity,“ A. Pinchera, S. H. Ingbar and J. M. McKenzie, eds., Plenum Press, New York (1987).Google Scholar
- 18.L. D. Kohn, W. A. Valente, E. F. Grollman, S. M. Aloj and P. Vitti, Clinical determination and/or quantification of thyrotropin and a variety of thyroid stimulatory or inhibitory factors performed in vitro with an improved thyroid cell line FRTL-5, U. S. Patent 4,609,622, Sep. 2 (1986).Google Scholar
- 21.F. S. Ambesi-Impiombato, Living, fast growing thyroid cell strain, FRTL-5, U. S. Patent 4,608,341, August 26 (1986).Google Scholar
- 22.L. D. Kohn, M. Saji, T. Akamizu, S. Ikuyama, O. Isozaki, A. D. Kohn, P. Santisteban, J. Y. Chan, S. Bellur, C. M. Rotella, F. V. Alvarez and S. M. Aloj, Receptors of the thyroid: the thyrotropin receptor is only the first violinist of a symphony orchestra, in: “The Thyroid: Regulation of its Normal Growth and Function,” R. Ekholm, L. D. Kohn and S. Wollman, eds., Plenum Press, New York (1989).Google Scholar
- 31.A. P. Feinberg and B. Vogelstein, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity, Anal. Biochem., 137: 266 (1984).Google Scholar