Immunocytochemistry and Synaptic Relationships of Physiologically Characterized HRP-Filled Neurons

  • Peter Somogyi
  • Tamás F. Freund


The combination of intracellular recording and marking of neurons with horseradish peroxidase (HRP) has greatly extended the understanding of neuronal circuits. As discussed in Chapter 7 by Kitai et al., this approach is suitable for the reconstruction of the axonal and dendritic arborizations of neurons over several millimeters, thus providing a more complete picture of individual cells than was possible with previous methods. In addition, the direct correlation of electrophysiological and structural data becomes possible. In most cases the interpretation of the possible role of identified neurons would benefit greatly from a knowledge of their biochemical characteristics, especially their transmitters. The identification of the transmitters of the postsynaptic cells would also be useful because each neuron or afferent system is likely to contact several neurochemically, morphologically, and/or physiologically distinct neuron populations.


Visual Cortex Pyramidal Cell Dendritic Spine Colloidal Gold Lateral Geniculate Nucleus 
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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Peter Somogyi
    • 1
  • Tamás F. Freund
    • 1
  1. 1.MRC Anatomical Neuropharmacology UnitUniversity Department of PharmacologyOxfordUK

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