Synaptic Relationships of Golgi-Impregnated Neurons as Identified by Electrophysiological or Immunocytochemical Techniques

  • Tamás F. Freund
  • P. Somogyi

Abstract

Much of our present knowledge about the cellular organization of the different brain areas derives from Golgi studies, dating back to the end of the last century when Golgi (1883), Ramon y Cajal (1891, 1911), and many others introduced the modern era of neuroanatomy. The Golgi method allows the visualization of a small proportion of the neurons present in a brain area, together with most of their dendritic and axonal processes. As a result the impregnated cells can be traced and reconstructed in great detail. To gain an overall view of the different cell types and the distribution of neuronal processes in a brain area, even today Golgi impregnation is often the method of choice. It can provide much valuable information regarding local circuit patterns provided the material is critically analyzed (Szentágothai, 1975; Szentágothai and Arbib, 1974; for review see Millhouse, 1981). One limitation of the Golgi technique is that generally synaptic interactions can only be predicted by indirect correlation of separately impregnated dendritic and axonal patterns. In some cases, e.g., in the cerebellum, this enabled the classical histologists to assemble correctly the wiring of the entire neuronal system, whereas in other cases the correspondence between separately impregnated pre- and postsynaptic processes is not so obvious as to predict the organization of neuronal circuits.

Keywords

Synaptic Contact Axon Initial Segment Dendritic Shaft Postsynaptic Target Potassium Dichromate Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Tamás F. Freund
    • 1
  • P. Somogyi
    • 1
  1. 1.MRC Anatomical Neuropharmacology UnitUniversity Department of PharmacologyOxfordUK

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