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Interchangeable Uses of Autoradiographic and Peroxidase Markers for Electron Microscopic Detection of Neuronal Pathways and Transmitter-Related Antigens in Single Sections

  • Chapter
Neuroanatomical Tract-Tracing Methods 2

Abstract

In the past several years, there has been a rapid expansion in use of immu-nocytochemical methods for identifying neuronal molecules. These include neurotransmitter-synthesizing enzymes, several transmitters such as norepinephrine (NE), dopamine (DA), 5-hydroxytryptamine (5-HT), and γ-aminobutyric acid (GABA), and a variety of neuropeptides (Cuello, 1983; Heym and Lang, 1986; Pickel, 1985; Polak and Van Noorden, 1983; Steinbush et al., 1978; Storm-Mathisen et al., 1983). The increasing use of immunocyto-chemistry is largely attributable to both commercial and individual production of a large number of mono- and polyclonal antibodies (MacMillan and Cuello, 1986; M. E. Ross et al., 1981; Sofroniew et al., 1978). Many of the available antibodies have a high titer and can be specifically localized by several different immunocytochemical methods. The most common methods employ secondary immunoglobulins (IgGs) produced against serum from the same species as the primary antibody. The secondary IgG is conjugated with either fluorescence, peroxidase, gold, or radiolabeled markers (i.e., tritium or iodine) for localization of the antibodies in sections of tissue (DeMey, 1983; Glazer et al., 1984; Hunt and Mantyh, 1984; Pickel et al., 1986). Alternatively, the secondary IgG can be immunologically bound to a peroxidase-antiperoxidase (PAP) complex with the antiperoxidase being produced in the same apecies as the primary antibody (Sternberger, 1979).

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  1. Department of Neurology and Neuroscience, Division of Neurobiology, Cornell University Medical College, New York, New York, 10021, USA

    Virginia M. Pickel & Teresa A. Milner

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  1. University of Virginia Medical Center, Charlottesville, Virginia, USA

    Lennart Heimer

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Pickel, V.M., Milner, T.A. (1989). Interchangeable Uses of Autoradiographic and Peroxidase Markers for Electron Microscopic Detection of Neuronal Pathways and Transmitter-Related Antigens in Single Sections. In: Heimer, L., Záborszky, L. (eds) Neuroanatomical Tract-Tracing Methods 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-2055-6_5

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