Microdissection in Combination with Biochemical Microassays as a Tool in Tract Tracing

  • Miklós Palkovits


During the last 15 years several brain microdissection techniques have been developed (see Cuello and Carson, 1983; Palkovits and Brownstein, 1983, 1988) and applied for a number of purposes, including (1) biochemical mapping for the distribution of various substances (neurotransmitters, hormones, enzymes, receptors, amino acids, etc.) in discrete brain nuclei, (2) measuring the concentrations of above substances in brain nuclei of animals following various treatments, experimental manipulations, or pathological conditions, (3) quantitative measurements to reveal the chemical specificity of neuronal interconnections or interactions between brain regions, (4) measuring the synthesis of various substances in individual brain nuclei following precursor injections into the brain, and (5) sampling of discrete brain nuclei for primary tissue cultures, subcellular analysis (synaptosomes), or for in vitro studies.


Brain Area Squirrel Monkey Brain Nucleus Neuronal Interconnection Fresh Brain 
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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Miklós Palkovits
    • 1
    • 2
  1. 1.First Department of AnatomySemmelweis University Medical SchoolBudapestHungary
  2. 2.Laboratory of Cell BiologyNational Institute of Mental HealthBethesdaUSA

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