Cytotoxic and Helper T-Lymphocyte Responses to Antibody Recognition Regions on Influenza Virus Hemagglutinin

  • M. Zouhair Atassi
  • Jose V. Torres
  • Philip R. Wyde


We have previously localized and synthesized twelve antibody recognition sites on influenza virus hemaggl uti ni n (HA). These peptides correspond to exposed surface areas in the 3-D structure of HA. Using intact X31 virus as the i mmnunogen, we have determined the recognition of these synthetic peptides by proliferative T-helper lymphocytes (ThL) , delayed type hypersensitivity (DTH) , and cytotoxic T-lymphocytes (CTL) responses. The responses to the individual determinants in each of these immune compartments were under separate Ir gene control. Conversely, using the peptides as immunogens, we have determined the ability of various peptide-specific antibodies (in outbred mice) and ThLs (in H-2k, H-2d, H-2s and H-2b mice) to recognize intact virus. Whereas most of the peptides primed the mice for an anti-peptide proliferative ThL response, only very few of these cross-reacted wi th the virus. The identity of the peptide(s) eliciting virus cross-reactive ThLs varied with the strain. The importance of antibody, ThL, CTL and DTH responses in protection against viral infection and in vaccine design is discussed.


Influenza Virus Tobacco Mosaic Virus Delay Type Hypersensitivity Antigenic Site Antigenic Structure 
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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • M. Zouhair Atassi
    • 1
    • 2
  • Jose V. Torres
    • 2
  • Philip R. Wyde
    • 2
  1. 1.Department of BiochemistryBaylor College of MedicineHoustonUSA
  2. 2.Department of Microbiology/ ImmunologyBaylor College of MedicineHoustonUSA

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