Developing a Gonococcal Protein I Vaccine
After many years of intensive research by numerous investigators, a safe and effective gonorrheal vaccine remains elusive. Our attention has focused on the outer membrane of the gonococcus and the potential advantages or disadvantages in the use of some of these gonococcal components for such a vaccine. The use of chromatographically purified products was selected over using boiled organisms (Braude et al., 1985) or delipidized gonococcal membrane vesiclesj Arminon et al., ( 1987; Buchanan and Arko, 1977) in order for us to monitor and assure the consistency of the vaccine. Reported here are some of our efforts on one such potential vaccine candidate, gonococcal protein I, and some of our studies on a minor but potentially hazardous contaminant, protein III.
KeywordsOuter Membrane Outer Membrane Protein Neisseria Gonorrhoeae Cyanogen Bromide Major Outer Membrane Protein
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- Arminjon, P., Cadoz, M., Morse, S.A., Rock, J.P. , and Sarafian, S.K. (1987). Bactericidal and opsonic activities of sera from individuals immunized with a gonococcal protein I vaccine. Abstracts Annual Meeting American Society of Microbiology 118.Google Scholar
- Braude, A., Wunderlich, A., Chikami, G., Douglas, H., and McCutchan, J. (1985). Generation of adherence-blocking immunoglobin G (IgG) by vaccinating women with nonpiliated boiled gonococci: Observations on IgG binding to a high-molecular-weight protein in the outer membrane fraction. In The Pathogenic Neisseriae, GK. Schoolnik, ed. (Washington D.C.: American Society of Microbiology), pp. 323–328.Google Scholar
- Joiner, K.A., Warren, K.A., Frank, M.M., and Rice, P.A. (1985a). Blocking immunoglobulin G enhances complement consumption and deposition on Neisseria gonorrhoeae. In The Pathogenic Neisseriae, GK. Schoolnik, ed. (Washington, D.C.: Amer. Soc. Microbiology), pp. 431–434.Google Scholar
- Lynch, E.C., Blake, M.S., Gotschlich, E.C., and Mauro, A. (1983). Reconstitution of a voltage-dependent anion-preferring porin from Neisseria gonorrhoeae. Biophys. J. 41, 62.Google Scholar
- Rice, P.A., Tam, M.R., and Blake, M.S. (1985). Immunoglobulin G antibodies in normal human serum directed against protein III block killing of serum-resistant Neisseria gonorrhoeae by immune human serum. In The Pathogenic Neisseriae, GK. Schoolnik, ed. (Washington, D.C.: Amer. Soc. Microbiology) , pp. 427–430.Google Scholar
- Sandstrom, E., and Buchanan, T.M. (1980). Coagglutination class reagents identifies the same antigen as the principal outer membrane serotyping. In Genetics and immunobiology of pathogenic Neisseria, S. Normark, and D. Danielsson, eds. (Umea: University of Umea), pp. 67–71.Google Scholar
- Young, J.D.E., Blake, M.S., Mauro, A., and Cohn, Z.A. (1983). Properties of the major outer membrane protein from Neisseria gonorrhoeae incorporated into model lipid membranes. Biophysical J. 80, 3831–3835.Google Scholar